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Home cDNA Clone TrueORF All ATP6V0E1 ORF Clones
TrueORF Gold

ATP6V0E1 (NM_003945) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC215618 ATP6V0E1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1), 10µg
$380
In Stock
RC515618 ATP6V0E1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1), (200ug) $500 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC215618L2 Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1), mGFP tagged

$830 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for ATP6V0E1 (NM_003945)
cDNA Clone shRNA/siRNA Lysate Protein Antibody Service
TrueORF Data for RC215618
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 246 bp
Predicted Protein MW: 9.2 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Transmembrane
Protein Pathways: Oxidative phosphorylationMetabolic pathwaysVibrio cholerae infectionEpithelial cell signaling in Helicobacter pylori infection
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_003945.3, NP_003936
RefSeq Size: 894 RefSeq ORF: 246
Synonyms : ATP6H; ATP6V0E; M9.2; Vma21; Vma21p
LocusID: 8992 Cytogenetic: 5q35.1
Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008].

 

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