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Home cDNA Clone TrueORF All HIST3H3 ORF Clones
TrueORF Gold

HIST3H3 (NM_003493) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC219443 HIST3H3 (Myc-DDK-tagged)-Human histone cluster 3, H3 (HIST3H3), 10µg   
$390
In Stock
RG219443 HIST3H3 (GFP-tagged) - Human histone cluster 3, H3 (HIST3H3), (10ug) $430 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC519443 HIST3H3 (Myc-DDK-tagged)-Human histone cluster 3, H3 (HIST3H3), (200ug) $520 2 weeks
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG219443 HIST3H3 (GFP-tagged) - Human histone cluster 3, H3 (HIST3H3), (10ug)

$430 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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Also for HIST3H3 (NM_003493)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody
TrueORF Data for RC219443
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 411 bp
Predicted Protein MW: 15.3 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Systemic lupus erythematosus
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_003493.2, NP_003484 RefSeq Size: 481 RefSeq ORF: 411
Synonyms : H3.4; H3/g; H3FT; H3t
LocusID: 8290 Cytogenetic: 1q42
Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015].

 

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