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Home cDNA Clone TrueORF All MPI ORF Clones
TrueORF Gold

MPI (NM_002435) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC208134 MPI (Myc-DDK-tagged)-Human mannose phosphate isomerase (MPI), (10ug), 10µg
$510
In Stock
RC508134 MPI (Myc-DDK-tagged)-Human mannose phosphate isomerase (MPI), (200ug) $630 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC208134L2 Lenti ORF clone of Human mannose phosphate isomerase (MPI), mGFP tagged

$970
$720
2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for MPI (NM_002435)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC208134
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1272 bp
Predicted Protein MW: 46.5 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: ES Cell Differentiation/IPS
Protein Pathways: Fructose and mannose metabolismAmino sugar and nucleotide sugar metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_002435.1, NP_002426
RefSeq Size: 1771 RefSeq ORF: 1272
Synonyms : CDG1B; PMI; PMI1
LocusID: 4351 Cytogenetic: 15q24.1 Domains: PMI_typeI
Summary: Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014].

 

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