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Home cDNA Clone TrueORF All LDHB ORF Clones
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LDHB (NM_002300) Human cDNA ORF Clone

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Cat. No. Description Price Availability  
RC205074 LDHB (Myc-DDK-tagged)-Human lactate dehydrogenase B (LDHB), transcript variant 1, 10µg
$540
In Stock
RC505074 LDHB (Myc-DDK-tagged)-Human lactate dehydrogenase B (LDHB), transcript variant 1, (200ug) $660 2 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification
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The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC205074L1 Lenti ORF clone of Human lactate dehydrogenase B (LDHB), transcript variant 1 , Myc-DDK-tagged

$910 In Stock
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC205074
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1005 bp
Predicted Protein MW: 36.5 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Glycolysis / GluconeogenesisCysteine and methionine metabolismPyruvate metabolismPropanoate metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_002300.3, NP_002291
RefSeq Size: 1336 RefSeq ORF: 1005
Synonyms : HEL-S-281; LDH-B; LDH-H; LDHBD; TRG-5
LocusID: 3945 Cytogenetic: 12p12.2-p12.1 Domains: ldh_C
Summary: This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010].

 

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