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Home cDNA Clone TrueORF All GCLM ORF Clones
TrueORF Gold

GCLM (NM_002061) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC207224 GCLM (Myc-DDK-tagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM), 10µg
$480
In Stock
RC507224 GCLM (Myc-DDK-tagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM), (200ug) $580 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC207224L2 Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), mGFP tagged

910 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for GCLM (NM_002061)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC207224
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 825 bp
Predicted Protein MW: 30.5 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Glutathione metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_002061.2, NP_002052
RefSeq Size: 3074 RefSeq ORF: 825
Synonyms : GLCLR
LocusID: 2730 Cytogenetic: 1p22.1
Summary: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008].

 

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