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Home cDNA Clone TrueORF All CHD8 ORF Clones

CHD8 (NM_001170629) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC230753 CHD8 (Myc-DDK-tagged)-Human chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, 10µg   
$1940
1-2 weeks
RG230753 CHD8 (GFP-tagged) - Human chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, (10ug) $2130 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC530753 0 2 weeks
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Clone Modification
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The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG230753 CHD8 (GFP-tagged) - Human chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, (10ug)
$2130 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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TrueORF Data for RC230753
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 7746 bp
Predicted Protein MW: 291 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Wnt signaling pathway

Reference Data
RefSeq Explanation: NM_001170629.1, NP_001164100 RefSeq Size: RefSeq ORF: 7746
Synonyms : AUTS18; HELSNF1
LocusID: 57680 Cytogenetic: 14q11.2
Gene Summary: This gene encodes a DNA helicase that functions as a transcription repressor by remodeling chromatin structure. It binds beta-catenin and negatively regulates Wnt signaling pathway, which plays a pivotal role in vertebrate early development and morphogenesis. Mice lacking this gene exhibit early embryonic death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010].

 

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