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Home cDNA Clone TrueORF All AMT ORF Clones

AMT (NM_001164710) Human cDNA ORF Clone

Western Blot Validated

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC228289 AMT (Myc-DDK-tagged)-Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug), 10µg
$430
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC228289L2 Lenti-ORF clone of AMT (mGFP-tagged)-Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

$880
$630
2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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TrueORF Data for RC228289
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1080 bp
Predicted Protein MW: 39.1 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Glycine, serine and threonine metabolismOne carbon pool by folateNitrogen metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_001164710.1, NP_001158182
RefSeq Size: RefSeq ORF: 1080
Synonyms : GCE; GCST; GCVT; NKH
LocusID: 275 Cytogenetic: 3p21.2
Summary: This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

 

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