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Home cDNA Clone TrueORF All ATP2B2 ORF Clones
TrueORF Gold

ATP2B2 (NM_001001331) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC221710 ATP2B2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1, 10µg
$1790
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC221710L2 Lenti ORF clone of Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1, mGFP tagged

$2380 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for ATP2B2 (NM_001001331)
cDNA Clone shRNA/siRNA Lysate Protein Antibody Service
TrueORF Data for RC221710
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 3732 bp
Predicted Protein MW: 136.7 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: TransmembraneDruggable Genome
Protein Pathways: Calcium signaling pathway
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_001001331.1, NP_001001331
RefSeq Size: 6821 RefSeq ORF: 3732
Synonyms : PMCA2; PMCA2a; PMCA2i
LocusID: 491 Cytogenetic: 3p25.3
Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

 

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