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Home cDNA Clone TrueORF All ALAS1 ORF Clones
TrueORF Gold

ALAS1 (NM_000688) Human cDNA ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC219938 ALAS1 (Myc-DDK-tagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, 10µg
1-2 weeks
RC519938 ALAS1 (Myc-DDK-tagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, (200ug) $660 3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC219938L1 Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1 , Myc-DDK-tagged

In Stock
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
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Also for ALAS1 (NM_000688)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody
TrueORF Data for RC219938
Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1923 bp
Predicted Protein MW: 70.4 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Glycine, serine and threonine metabolismPorphyrin and chlorophyll metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_000688.4, NP_000679
RefSeq Size: 2407 RefSeq ORF: 1923
LocusID: 211 Cytogenetic: 3p21.1 Domains: ALA_synthase, aminotran_1_2
Summary: This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011].


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