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Home cDNA Clone TrueORF All ALAD ORF Clones
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ALAD (NM_000031) Human cDNA ORF Clone

Specifications Citations (1) Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC201545 ALAD (Myc-DDK-tagged)-Human aminolevulinate dehydratase (ALAD), 10µg   
In Stock
RG201545 ALAD (GFP-tagged) - Human aminolevulinate dehydratase (ALAD), (10ug) $530 2-3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Cat. No. Description Price Availability
RC501545 0 4 weeks
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Clone Modification

The same insert cloned in Lenti and other vectors are also available.

Cat. No. Description Vector Price Availability  
RC201545L1 Lenti ORF clone of Human aminolevulinate dehydratase (ALAD) , Myc-DDK-tagged

$660 3 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $390 In Stock
Lenti particle icon Add to Shopping Cart
TrueORF Data for RC201545
Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1020 bp
Predicted Protein MW: 37.23 kDa
Restriction Sites: SgfI-RsrII     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Druggable Genome
Protein Pathways: Porphyrin and chlorophyll metabolismMetabolic pathways
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq Explanation: NM_000031.4, NP_000022 RefSeq Size: 3151 RefSeq ORF: 993
Synonyms : ALADH; PBGS
LocusID: 210 Cytogenetic: 9q33.1 Domains: ALAD
Gene Summary: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015].


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