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Home cDNA Clone TrueORF All IDH1 ORF Clones

IDH1 (NM_005896) Human Mutant ORF Clone

Specifications Citations Clones of Other Species Product Documents
Cat. No. Description Price Availability  
RC400097 IDH1 mutant (R132C), Myc-DDK-tagged ORF clone of Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) as transfection-ready DNA, 10µg
$680
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248 In Stock
Clone Modification
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Also for IDH1 (NM_005896)
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TrueORF Data for RC400097
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 1245 bp
Predicted Protein MW: 46.5 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map
OTI Annotation: Reference: IDH1 and IDH2 Mutations in GliomasN. Engl. J. Med., Feb 2009; 360: 765 - 773.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Citrate cycle (TCA cycle)Glutathione metabolismMetabolic pathways

Reference Data
RefSeq: , NP_005887
RefSeq Size: 2339 RefSeq ORF: 1245
Synonyms : HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
LocusID: 3417 Cytogenetic: 2q33.3 Domains: isodh
Summary: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013].

 

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