Angiomotin (AMOT) (NM_133265) Human Tagged ORF Clone

CAT#: RC220347

AMOT (Myc-DDK-tagged)-Human angiomotin (AMOT), transcript variant 2

Product Images

Circular map for RC220347

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AMOT (Cat# RC220347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMOT(Cat# TA507142). Positive lysates LY408854 (100ug) and LC408854 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY408854) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220347 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AMOT protein (Cat# TP320347). The protein was produced from HEK293T cells transfected with AMOT cDNA clone (Cat# RC220347) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Angiomotin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220347 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCGGGCTCAGCCATCCTCTGCTTCTTATCAGCCAGTGCCAGCAGACCCTTTTGCCATTGTTTCCA
  GAGCCCAGCAGATGGTTGAGATCCTCTCAGACGAGAACCGGAACTTGAGGCAAGAGTTGGAAGGATGCTA
  TGAGAAGGTGGCAAGACTGCAGAAGGTGGAGACAGAAATCCAGCGCGTCTCGGAGGCATATGAGAACCTC
  GTGAAGTCATCCTCCAAAAGAGAGGCCCTAGAGAAAGCCATGAGAAACAAGCTAGAGGGCGAGATTCGGA
  GGATGCATGATTTCAACAGGGATCTGAGAGAGCGTCTAGAGACTGCCAACAAGCAGCTTGCAGAGAAGGA
  ATATGAGGGGTCAGAGGACACCAGAAAAACCATCTCGCAGCTCTTTGCAAAAAATAAAGAAAGCCAGCGT
  GAGAAGGAGAAGCTGGAAGCGGAGCTGGCCACTGCCCGTTCTACCAATGAGGACCAAAGACGACACATCG
  AAATCCGAGATCAGGCCCTGAGTAATGCCCAGGCCAAGGTGGTAAAGCTGGAAGAAGAGCTGAAAAAGAA
  GCAAGTGTACGTTGACAAGGTGGAGAAGATGCAGCAGGCCCTTGTACAGCTCCAGGCAGCATGTGAAAAA
  CGTGAGCAGCTAGAGCACCGTCTCCGGACACGACTGGAGAGGGAACTGGAATCCCTGAGAATCCAGCAGC
  GTCAGGGCAACTGTCAGCCCACCAACGTTTCAGAATACAATGCTGCTGCACTGATGGAGCTCCTTCGGGA
  GAAAGAGGAGAGGATTCTGGCTCTGGAAGCTGATATGACAAAGTGGGAGCAGAAATATTTGGAGGAGAAT
  GTGATGAGACATTTTGCTCTGGATGCTGCTGCAACTGTGGCTGCTCAGAGGGACACAACAGTCATCAGTC
  ACTCTCCTAACACCAGCTATGACACAGCTCTAGAAGCTCGCATCCAGAAAGAGGAGGAAGAAATCTTGAT
  GGCCAATAAGCGTTGCCTTGACATGGAGGGCAGGATTAAGACCCTCCATGCCCAGATTATTGAGAAGGAT
  GCCATGATCAAAGTACTCCAGCAGCGTTCCCGGAAGGAGCCGAGCAAGACAGAGCAGCTGTCGTGCATGC
  GGCCAGCGAAGTCTCTGATGTCCATTTCCAATGCTGGATCAGGCTTGCTCTCCCACTCATCCACCCTGAC
  TGGCTCCCCCATCATGGAAGAAAAGCGAGACGACAAGAGCTGGAAGGGGAGCCTAGGCATTCTCCTGGGT
  GGAGACTACCGTGCTGAATATGTCCCTTCCACACCCTCGCCTGTGCCACCCTCGACTCCCCTGCTCTCGG
  CTCACTCCAAGACAGGCAGCCGAGACTGCAGTACCCAAACTGAACGTGGGACGGAATCGAACAAAACTGC
  AGCTGTTGCTCCCATCTCTGTTCCTGCTCCAGTTGCTGCTGCCGCCACTGCTGCCGCCATCACTGCCACT
  GCTGCCACCATCACCACCACCATGGTAGCTGCTGCTCCAGTTGCTGTTGCTGCTGCTGCTGCTCCAGCTG
  CTGCTGCTGCCCCGTCTCCAGCCACTGCCGCTGCTACTGCTGCTGCTGTTTCTCCAGCTGCTGCTGGTCA
  GATTCCAGCTGCTGCCTCTGTTGCCTCAGCTGCTGCCGTTGCTCCTTCTGCTGCTGCTGCTGCTGCTGTT
  CAGGTTGCTCCAGCTGCTCCGGCTCCAGTTCCAGCTCCGGCTCTGGTTCCGGTTCCAGCTCCAGCAGCGG
  CTCAGGCTTCTGCTCCTGCTCAGACTCAGGCACCAACTTCAGCTCCGGCTGTGGCTCCAACTCCAGCTCC
  AACTCCAACTCCAGCTGTGGCTCAGGCTGAGGTTCCTCCTGCAAGTCCAGCTACCGGTCCTGGACCACAT
  CGTTTGTCTATACCAAGTTTGACCTGCAATCCAGACAAAACAGATGGGCCTGTGTTCCACTCCAATACTC
  TGGAAAGAAAAACTCCCATTCAGATCCTGGGACAAGAGCCTGATGCAGAGATGGTGGAATATCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220347 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPRAQPSSASYQPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENL
  VKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQR
  EKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEK
  REQLEHRLRTRLERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEEN
  VMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKD
  AMIKVLQQRSRKEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLG
  GDYRAEYVPSTPSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITAT
  AATITTTMVAAAPVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAV
  QVAPAAPAPVPAPALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPPASPATGPGPH
  RLSIPSLTCNPDKTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133265
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133265.3
• RefSeq Size: 6514 bp
• RefSeq ORF: 2028 bp
• Locus ID: 154796
• UniProt ID: Q4VCS5
• Cytogenetics: Xq23
• MW: 72.6 kDa
• Gene Summary: This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]