Xpnpep2 (NM_133213) Mouse Tagged ORF Clone

CAT#: MR213518

Xpnpep2 (Myc-DDK-tagged) - Mouse X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound (Xpnpep2), transcript variant 1

Product Images

Circular map for MR213518

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Xpnpep2
• Synonyms: 9030008G12Rik; mAPP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213518 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAAGCCTACTGGCAGTGCTACCCCTGGCTTATTCTCCTCTGTGCATGTGCCTGGAGCTACCCAG
  AGCCCAAGTACCTTGGAAGGGAAGATGTGAGAAACTGTTCAACCAGCCCTGAGCGCCTGCCAGTTACTGC
  CGTCAATACAACAATGCGGCTTGCAGCCCTCCGCCAACAGATGGAGACCTGGAATCTCTCTGCCTACATC
  ATCCCAGACACGGATGCACACATGAGTGAGTACATTGGCAAACCTGATAAGAGGCGAGAGTGGATTTCAG
  GCTTCACAGGGTCTGCAGGTACTGCTGTGGTGACCATGGGGAAAGCAGCTGTCTGGACCGATAGTCGTTA
  CTGGACTCAGGCCGAAAGGCAGATGGACTGCAACTGGGAGCTGCACAAGGAAGTTAGCATTTCTTCCATT
  GTCGCCTGGATCTTAGCTGAAGTCCCTGATGGACAGAACGTGGGCTTCGACCCCTTCCTCTTTTCTGTTG
  ATTCCTGGAAAAATTATGATCAAGGATTCCAAGACTCCAGCAGACACCTGTTATCGGTTACAACCAACCT
  TGTTGACGTGGCATGGGGGTCAGAGAGGCCCCCAGTGCCAAGCCAACCCATTTATGCCCTGCCGAAAGAA
  TTTACAGGGAGCACTTGGCAGGAAAAAGTATCCGCTGTCCGAAGCTATATGGAGCACCATGCCAAGACTC
  CAACTGGGGTCCTTCTATCTGCACTTGATGAGACAGCCTGGCTCTTCAACCTTCGTAGCAGTGACATCCC
  CTATAACCCCTTCTTCTACTCCTATGCACTGCTCACGAACTCCTCCATCAGGTTGTTTGTCAACAAGAGT
  CGCTTTAGCCTTGAGACCTTGCAGTACCTGAATACAAACTGCACATTACCCATGTGTGTGCAACTTGAGG
  ACTACAGTCAAGTTCGTGACAGTGTGAAGGCCTATGCCTCAGGCGATGTGAAAATCTTAATTGGGGTCAG
  CTATACCACCTATGGGGTCTATGAAGTGATACCCAAGGAGAAACTCGTGACAGACACCTACTCCCCAGTG
  ATGTTAATCAAGGCTGTGAAGAACAGCAAGGAGCAGGCCCTTTTGAAGAGCAGCCACGTGCGGGACGCTG
  TGGCTGTGATCCAGTACTTGGTCTGGTTGGAGAAGAACGTGCCCAAAGGCACGGTGGATGAGTTTTCTGG
  GGCAGAATACATTGATGAGTTACGACGGAATGAAAACTTCTCCTCTGGACCCAGTTTTGAAACCATCTCT
  GCTAGTGGCCTGAATGCCGCCCTGGCCCATTACAGCCCAACAAAAGAGCTGCACCGTAAGCTGTCCTCAG
  ATGAGATGTACCTGGTGGATTCTGGGGGGCAATACTGGGATGGGACCACAGATATCACCAGAACAGTACA
  TTGGGGCACTCCTACTGCCTTCCAAAAGGAGGCCTATACTCGAGTGCTAATGGGAAACATCGATCTGTCC
  AGACTTGTCTTTCCTGCTGCTACATCAGGGAGAGTGATAGAAGCCTTTGCCCGAAGAGCCTTATGGGAAG
  TTGGGCTCAATTATGGTCATGGGACAGGCCATGGCATTGGCAACTTCCTCTGTGTGCATGAGTGGCCAGT
  GGGATTCCAGTATAACAACATTGCCATGGCCAAGGGCATGTTCACTTCCATTGAACCTGGATACTACCAT
  GATGGGGAGTTTGGAATCCGTCTTGAAGATGTCGCCCTTGTGGTAGAAGCAAAAACCAAGTACCCAGGGG
  ACTACCTGACTTTTGAATTGGTGTCCTTTGTGCCCTATGACCGAAACCTCATCGATGTCAGGCTGCTGTC
  CCCAGAGCAGCTCCAGTACCTGAACCGCTACTATCAGACCATTCGTGAGAATGTAGGCCCAGAGCTGCAG
  CGTCGCCAGCTGCTGGAGGAGTTTGCATGGTTGGAGCAGCACACGGAGCCCCTGTCAGCCAGGGCCCCTC
  ATATCATCTCCTGGACCTCTCTGTGGGTGGCCTCTGCCCTTGCCATCCTCAGCTGGAGTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213518 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQAYWQCYPWLILLCACAWSYPEPKYLGREDVRNCSTSPERLPVTAVNTTMRLAALRQQMETWNLSAYI
  IPDTDAHMSEYIGKPDKRREWISGFTGSAGTAVVTMGKAAVWTDSRYWTQAERQMDCNWELHKEVSISSI
  VAWILAEVPDGQNVGFDPFLFSVDSWKNYDQGFQDSSRHLLSVTTNLVDVAWGSERPPVPSQPIYALPKE
  FTGSTWQEKVSAVRSYMEHHAKTPTGVLLSALDETAWLFNLRSSDIPYNPFFYSYALLTNSSIRLFVNKS
  RFSLETLQYLNTNCTLPMCVQLEDYSQVRDSVKAYASGDVKILIGVSYTTYGVYEVIPKEKLVTDTYSPV
  MLIKAVKNSKEQALLKSSHVRDAVAVIQYLVWLEKNVPKGTVDEFSGAEYIDELRRNENFSSGPSFETIS
  ASGLNAALAHYSPTKELHRKLSSDEMYLVDSGGQYWDGTTDITRTVHWGTPTAFQKEAYTRVLMGNIDLS
  RLVFPAATSGRVIEAFARRALWEVGLNYGHGTGHGIGNFLCVHEWPVGFQYNNIAMAKGMFTSIEPGYYH
  DGEFGIRLEDVALVVEAKTKYPGDYLTFELVSFVPYDRNLIDVRLLSPEQLQYLNRYYQTIRENVGPELQ
  RRQLLEEFAWLEQHTEPLSARAPHIISWTSLWVASALAILSWSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133213
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133213.1, NM_133213.2, NP_573476.2
• RefSeq Size: 3498 bp
• RefSeq ORF: 2025 bp
• Locus ID: 170745
• UniProt ID: B1AVD1
• Cytogenetics: X A4
• MW: 76.4 kDa
• Gene Summary: Membrane-bound metalloprotease which catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. May play a role in the metabolism of the vasodilator bradykinin.[UniProtKB/Swiss-Prot Function]