Ptger1 (NM_013641) Mouse Tagged ORF Clone

CAT#: MR225549

Ptger1 (Myc-DDK-tagged) - Mouse prostaglandin E receptor 1 (subtype EP1) (Ptger1)

Product Images

Circular map for MR225549

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptger1
• Synonyms: 42kDa; EP1; Ptgerep1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225549 representing NM_013641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCTGCGGGCTTAACCTGAGCCTAGCGGATGAGGCAGCAACGTGCGCAACACCCAGGCTCCCCA
  ATACATCTGTGGTGCTGCCAACAGGCGATAATGGCACATCACCAGCGCTGCCTATCTTCTCCATGACGCT
  GGGTGCTGTGTCCAACGTGCTGGCGCTGGCGCTGCTGGCCCAGGTTGCAGGCCGAATGCGGCGGCGCCGC
  TCGGCTGCCACCTTCCTGTTGTTCGTCGCCAGCCTGCTTGCCATCGACCTAGCAGGCCATGTGATCCCGG
  GCGCCCTGGTGCTTCGCCTGTATACTGCGGGGCGTGCGCCTGCTGGAGGGGCGTGTCATTTCCTGGGTGG
  CTGCATGGTCTTCTTCGGCCTGTGCCCACTTTTGCTTGGCTGTGGCATGGCCGTAGAGCGCTGTGTGGGT
  GTCACGCAGCCACTGATCCACGCGGCGCGCGTATCTGTGGCCCGCGCACGCCTGGCACTAGCCGTGCTGG
  CTGCCATGGCTTTGGCAGTGGCGCTGCTGCCACTGGTACACGTGGGTCGCTACGAGTTACAGTACCCTGG
  CACCTGGTGTTTTATTAGCCTTGGGCCTCGTGGAGGTTGGCGCCAGGCGTTGCTTGCCGGCCTCTTCGCC
  GGCCTTGGCCTGGCCGCGCTCCTTGCGGCATTAGTGTGCAATACGCTCAGCGGCCTGGCGCTCCTCCGTG
  CCCGCTGGAGACGGCGTCGCTCTCGACGATTCCGAAAGACCGCAGGTCCCGATGATCGCCGGCGCTGGGG
  ATCTCGTGGACCCCGCTTGGCCTCCGCCTCGTCTGCCTCATCCATCACTTCAGCCACAGCCACCCTCCGC
  AGCTCTCGGGGCGGCGGCTCCGCGCGCAGGGTTCACGCACACGATGTGGAAATGGTGGGCCAGCTCGTGG
  GCATCATGGTGGTGTCGTGCATCTGCTGGAGCCCCCTGCTGGTGTTGGTGGTGTTGGCCATCGGGGGCTG
  GAACTCTAACTCCCTGCAGCGGCCACTCTTTCTGGCTGTACGCCTCGCATCGTGGAACCAGATCCTGGAC
  CCATGGGTGTACATCCTGCTGCGCCAGGCCATGCTGCGCCAACTGCTTCGCCTCCTACCCCTGAGGGTCA
  GTGCCAAGGGTGGTCCAACGGAGCTGGGCCTAACCAAGAGTGCCTGGGAAGCCAGTTCACTGCGTAGTTC
  CCGGCACAGTGGTTTCAGTCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225549 representing NM_013641
  Red=Cloning site Green=Tags(s)
  
  MSPCGLNLSLADEAATCATPRLPNTSVVLPTGDNGTSPALPIFSMTLGAVSNVLALALLAQVAGRMRRRR
  SAATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGGACHFLGGCMVFFGLCPLLLGCGMAVERCVG
  VTQPLIHAARVSVARARLALAVLAAMALAVALLPLVHVGRYELQYPGTWCFISLGPRGGWRQALLAGLFA
  GLGLAALLAALVCNTLSGLALLRARWRRRRSRRFRKTAGPDDRRRWGSRGPRLASASSASSITSATATLR
  SSRGGGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGWNSNSLQRPLFLAVRLASWNQILD
  PWVYILLRQAMLRQLLRLLPLRVSAKGGPTELGLTKSAWEASSLRSSRHSGFSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013641
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013641.3
• RefSeq Size: 2082 bp
• RefSeq ORF: 1218 bp
• Locus ID: 19216
• UniProt ID: P35375
• Cytogenetics: 8 40.22 cM
• MW: 43.4 kDa
• Gene Summary: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues.[UniProtKB/Swiss-Prot Function]