Ppard (NM_011145) Mouse Tagged ORF Clone

CAT#: MR207001

Ppard (Myc-DDK-tagged) - Mouse peroxisome proliferator activator receptor delta (Ppard)

Product Images

Circular map for MR207001

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppard
• Synonyms: Nr1c2; NUC-1; NUC1; PPAR-beta; PPAR-delta; Pparb; Pparb/d; PPARdelta; PPAR[b]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207001 representing NM_011145.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAACAGCCACAGGAGGAGACCCCTGAGGCCCGGGAAGAGGAGAAAGAGGAAGTGGCCATGGGTGAC
  GGAGCCCCGGAGCTCAATGGGGGACCAGAACACACGCTTCCTTCCAGCAGCTGTGCAGACCTCTCCCAG
  AATTCCTCCCCTTCCTCCCTGCTGGACCAGCTGCAGATGGGCTGTGATGGGGCCTCAGGCGGCAGCCTC
  AACATGGAATGTCGGGTGTGCGGGGACAAGGCCTCGGGCTTCCACTACGGGGTCCACGCGTGCGAGGGG
  TGCAAGGGCTTCTTCCGCCGGACAATCCGCATGAAGCTCGAGTATGAGAAGTGCGATCGGATCTGCAAG
  ATCCAGAAGAAGAACCGCAACAAGTGTCAGTACTGCCGCTTCCAGAAGTGCCTGGCACTCGGCATGTCG
  CACAACGCTATCCGCTTTGGACGGATGCCGGAGGCCGAGAAGAGGAAGCTGGTGGCGGGGCTGACTGCC
  AGCGAGGGGTGCCAGCACAACCCCCAGCTGGCCGACCTGAAGGCCTTCTCTAAGCACATCTACAACGCC
  TACCTGAAAAACTTCAACATGACCAAAAAGAAGGCCCGGAGCATCCTCACCGGCAAGTCCAGCCACAAC
  GCACCCTTTGTCATCCACGACATCGAGACACTGTGGCAGGCAGAGAAGGGCCTGGTGTGGAAACAGCTG
  GTGAACGGGCTGCCGCCCTACAACGAGATCAGTGTGCACGTGTTCTACCGCTGCCAGTCCACCACAGTG
  GAGACAGTCCGAGAGCTCACCGAGTTCGCCAAGAACATCCCCAACTTCAGCAGCCTCTTCCTCAATGAC
  CAGGTGACCCTCCTCAAGTATGGCGTGCACGAGGCCATCTTTGCCATGCTGGCCTCCATCGTCAACAAA
  GACGGGCTGCTGGTGGCCAACGGCAGTGGCTTCGTCACCCACGAGTTCTTGCGAAGTCTCCGCAAGCCC
  TTCAGTGACATCATTGAGCCCAAGTTCGAGTTTGCTGTCAAGTTCAATGCGCTGGAGCTCGATGACAGT
  GACCTGGCGCTCTTCATCGCGGCCATCATTTTGTGTGGAGACCGGCCAGGCCTCATGAATGTGCCCCAG
  GTAGAAGCCATCCAGGACACCATTCTGCGGGCTCTAGAATTCCATCTGCAGGTCAACCACCCTGACAGC
  CAGTACCTCTTCCCCAAGCTGCTGCAGAAGATGGCAGACCTGCGGCAGCTGGTCACTGAGCATGCCCAG
  ATGATGCAGTGGCTAAAGAAGACGGAGAGTGAGACCTTGCTGCACCCCCTGCTCCAGGAAATCTACAAG
  GACATGTAC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR207001
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEQPQEETPEAREEEKEEVAMGDGAPELNGGPEHTLPSSSCADLSQNSSPSSLLDQLQMGCDGASGGSL
  NMECRVCGDKASGFHYGVHACEGCKGFFRRTIRMKLEYEKCDRICKIQKKNRNKCQYCRFQKCLALGMS
  HNAIRFGRMPEAEKRKLVAGLTASEGCQHNPQLADLKAFSKHIYNAYLKNFNMTKKKARSILTGKSSHN
  APFVIHDIETLWQAEKGLVWKQLVNGLPPYNEISVHVFYRCQSTTVETVRELTEFAKNIPNFSSLFLND
  QVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTHEFLRSLRKPFSDIIEPKFEFAVKFNALELDDS
  DLALFIAAIILCGDRPGLMNVPQVEAIQDTILRALEFHLQVNHPDSQYLFPKLLQKMADLRQLVTEHAQ
  MMQWLKKTESETLLHPLLQEIYKDMY
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using MR207001 also available, TP507001
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011145
• ORF Size: 1320 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011145.2
• RefSeq Size: 3240 bp
• RefSeq ORF: 1323 bp
• Locus ID: 19015
• UniProt ID: P35396
• Cytogenetics: 17 14.64 cM
• MW: 49.7 kDa
• Gene Summary: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand (By similarity).[UniProtKB/Swiss-Prot Function]