The OriGene Protein Collection is provided to our customers to further address their needs for a complete experimental solution encompassing cDNAs for overexpression, shRNA for underexpression, antibodies for detection and now proteins for functional evaluation. The OriGene Protein Collection is provided by experts in this complex field.
Functionally active proteins can be overexpressed in and purified from several different systems that have been well characterized. OriGene's manufacturer primarily utilizes bacterial and baculoviral systems and has produced over 100 transcription and cancer related proteins.
OriGene, through its partnership with ProteinOne, is actively preparing functional proteins every day for our catalog and to address our customer's bulk protein needs. Customers are encouraged to email and request that their protein of interest be placed on the priority list.
Whereas a human gene transcript supplies the required information to blueprint the protein sequence, in order to test cellular function, it is necessary to produce and test the actual protein. OriGene has partnered with quality, human protein providers who derive value from OriGenes large transcript repository. These high-quality protein samples are made accessible through this website.
Enzymatic Assays: Protein enzymes can be used to catalyze reactions of interest in search of substrate specificity. The researcher can also use such assays to assess both activation and inhibition of the enzymes activity. OriGene provides many quality protein kinases to test their phosphorylation activity.
Protein Binding Studies: Having a properly folded protein is key to determine its biologically relevant interactions. This type of study is traditionally performed through gel-shift assays, ELISA plate analysis, and various types of affinity chromatography.
Western Blots and SDS-PAGE: The identification of a proteins rate of migration (Mr) through SDS-PAGE is best identified through the use of a purified protein. The proteins available through OriGene are suitable for this purpose.
Functional Studies: In addition to enzymatic or binding assays, the researcher can uncover the cellular function of these proteins through a variety of specialized assays measuring fluctuations in second messengers calcium or camp. Transcriptional regulation can be assessed through off-the-shelf reporter gene assays.
Functionally active proteins are over expressed in and purified from several different systems that have been well characterized. ProteinOne primarily utilizes bacterial and baculoviral systems to produce proteins with a focus on transcription and cancer related proteins. The protein purification strategy revolves around utilizing protein tags including 6-histidine, FLAG or myc, to increase purify of the proteins to near homogeneity upon initial purification. Initial synthesis is the creation of new plasmids containing one or more of the protein tags that do not interfere with the protein function(s). This is followed by a second step of purification (required to further purify recombinant proteins even with a unique protein tag), which will be decided based on protein structure and property. These steps involve use of conventional chromatography, fast performance liquid chromatography (FPLC) and / or high-pressure liquid chromatography (HPLC) based techniques to achieve highly purified proteins. Quality is monitored by SDS-PAGE.
SDS-PAGE of Topo I (C651-765) Human DNA Topoisomerase I (C terminal domain)
ProteinOne proteins are purified greater than 95% homogeneous and contain no detectable protease, DNase, and RNase activity.