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Browse all proteins
OriGene lysate in recent publications |
Anti-TIF1 antibodies (anti-p155) in adult patients with dermatomyositis: comparison of different diagnostic assays
Ann Rheum Dis, Jan 2012; 10.1136/annrheumdis-2011-200871.
[TRIM33
]
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Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis
J. Biol. Chem., Dec 2011; 286: 44799 - 44810.
[RELA]
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Inhibitory Member of the Apoptosis-stimulating Proteins of the p53 Family (iASPP) Interacts with Protein Phosphatase 1 via a Noncanonical Binding Motif
J. Biol. Chem., Dec 2011; 286: 43039 - 43044.
[PPP1CB]
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Phospholipase D2 (PLD2) is a guanine nucleotide exchange factor (GEF) for the GTPase Rac2
PNAS, Dec 2011; 108: 19617 - 19622.
[VAV1]
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AKT1 Over-expression Lysate Product
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![Bioactivity Image]() |
| Cat. # |
Description |
Amount |
Price |
Shipping |
| LY423056 |
Antigen standard for v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3 (transient overexpression lysate) |
100 ug |
$280 |
5 days |
| LY40001 |
Empty vector transfected control cell lysate (HEK293 cell lysate) |
100 ug |
Included for Free |
|
|
| Expression cell line: |
Human HEK293T cell line |
| Expression cDNA Clone: |
TrueORF Clone RC201850 |
| Epitope tag: | C-terminal Myc/DDK* |
| Detection Antibodies: | Anti-DDK Antibody (TA50011) |
| Application: |
Western Blot experiments.
Left-Control; Right -Over-expression Lysate |
| Accession Number: | NM_001014431 NP_001014431 |
| Other Names: | AKT1,AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
| Bioactivity: |
view data |
| Predicted MW: | 55.5 kDa |
| Components: | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol) |
| Storage: | The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Preparation: | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20ºC before shipping.
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| Protein Families: |
Protein KinaseES Cell Differentiation/IPSDruggable Genome |
| Protein Pathways: |
MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >> |
*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich
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