Home Over-expression Lysates CAST Lysate|
CAST Over-expression Lysate Product
Also for CAST (NM_001750)
|Expression cell line:
||Human HEK293T cell line
|Expression cDNA Clone:
||TrueORF Clone RC211776|
|Epitope tag:||C-terminal Myc/DDK*|
|Detection Antibodies:||Anti-DDK Antibody (TA50011)|
||Western Blot experiments.
Left-Control; Right -Over-expression Lysate
|Accession Number:||NM_001750 NP_001741|
|Other Names:||CAST,BS-17; MGC9402; MGC9402; OTTHUMP00000158519; OTTHUMP00000158520; calpain inhibitor; heart-type calpastatin; sperm BS-17 component; calpastatin
|Predicted MW:||84.8 kDa|
|Components:||1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer|
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol)
|Storage:||The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles. |
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
|Preparation:||HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20ºC before shipping.
*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich