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Home Over-expression Lysates GLRA2 Lysate

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GLRA2 Over-expression Lysate Product

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Cat. # Description Price Availability
LC426528
  • GLRA2 HEK293T cell transient overexpression lysate (as WB positive control), 20ug (for larger package size, click here)
$50 In Stock
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Expression cell line:Human HEK293T cell line
Expression cDNA Clone:TrueORF Clone RC225747
Epitope tag:C-terminal Myc/DDK*
Detection Antibodies:Anti-DDK Antibody (TA50011)
Accession Number:NM_001118886   NP_001112358
Other Names:GLRA2,GLR; GLR
Predicted MW: kDa
Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage: The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Protein Families: TransmembraneIon Channels: Cys-loop ReceptorsDruggable Genome
Protein Pathways: Neuroactive ligand-receptor interaction

 

*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich
**: Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping

 

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