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Home Over-expression Lysates EPHA1 Lysate

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EPHA1 Over-expression Lysate Product

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Specifications Citations Related Products Product Documents
Cat. # Description Price Availability
LC401608
  • EPHA1 HEK293T cell transient overexpression lysate (as WB positive control), 20ug (for larger package size, click here)
$50 In Stock
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Also for EPHA1 (NM_005232)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

Expression cell line:Human HEK293T cell line
Expression cDNA Clone:TrueORF Clone RC213689
Epitope tag:C-terminal Myc/DDK*
Detection Antibodies:Anti-DDK Antibody (TA50011)
Accession Number:NM_005232   NP_005223
Other Names:EPHA1,EPH; EPHT; EPHT1
Predicted MW:107.9 kDa
Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage: The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Protein Families: Protein KinaseTransmembraneDruggable Genome
Protein Pathways: Axon guidance

 

*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich
**: Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping

 

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