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Home Antibody ELISA Kits

Mouse Cathepsin D ELISA Kit

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SKU Description Manual Price Availability*  
EA100431 Mouse Cathepsin D ELISA Kit Manual $400 2-3 Weeks Add to Shopping Cart

Specification


Product NameMouse Cathepsin D ELISA Kit
Size1x96 well plate
Gene ID13033
Gene SymbolCtsd
Assay TypeSandwich ELISA kit of Quantitative Detection for Mouse Cathepsin D
SignalColorimetric
Format8×12 divisible strips
Assay Length3.5 hours incubations; 1 hour washing and analyzing samples
SpecificityThis kit is used for quantitative detection of mouse Cathepsin D
ReactivityMouse
Cross ReactivityThere is no detectable cross-reactivity with other relevant proteins.
InterferenceNo significant interference observed with available related molecules.
Sample Volume100 ul
Sample TypeCell culture supernates, serum and plasma(heparin, EDTA).
Sensitivity<10pg/ml
Curve Range156pg/ml-10,000pg/ml
BackgroundCathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Components
  • 96-well plate precoated with anti- mouse Cathepsin D antibody 1
  • Lyophilized recombinant mouse Cathepsin D standard 10ng/tubex2
  • Biotinylated anti- mouse Cathepsin D antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Standard Curve spacer

The standard curve may vary based on the QC data of each batch. Please refer to the actual datasheet along with shipment.

 

 

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