IL1RA (IL1RN) (NM_173841) Human Untagged Clone

CAT#: SC306938

IL1RN (untagged)-Human interleukin 1 receptor antagonist (IL1RN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL1RA
• Synonyms: DIRA; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1F3; IL1RA; IRAP; MVCD4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_173841 edited
  GAAGACCTCCTGTCCTATCAGGCCCTCCCCATGGCTTTAGCTGACTTGTATGAAGAAGGA
  GGTGGAGGAGGAGGAGAAGGTGAAGACAATGCTGACTCAAAGGAGACGATCTGCCGACCC
  TCTGGGAGAAAATCCAGCAAGATGCAAGCCTTCAGAATCTGGGATGTTAACCAGAAGACC
  TTCTATCTGAGGAACAACCAACTAGTTGCCGGATACTTGCAAGGACCAAATGTCAATTTA
  GAAGAAAAGATAGATGTGGTACCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGA
  GGGAAGATGTGCCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCA
  GTTAACATCACTGACCTGAGCGAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGC
  TCAGACAGTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGC
  ACAGCGATGGAAGCTGACCAGCCCGTCAGCCTCACCAATATGCCTGACGAAGGCGTCATG
  GTCACCAAATTCTACTTCCAGGAGGACGAGTAGTACTGCCCAGGCCTGCCTGTTCCCATT
  CTTGCATGGCAAGGACTGCAGGGACTGCCAGTCCCCCTGCCCCAGGGCTCCCGGCTATGG
  GGGCACTGAGGACCAGCCATTGAGGGGTGGACCCTCAGAAGGCGTCACAACAACCTGGTC
  ACAGGACTCTGCCTCCTCTTCAACTGACCAGCCTCCATGCTGCCTCCAGAATGGTCTTTC
  TAATGTGTGAATCAGAGCACAGCAGCCCCTGCACAAAGCCCTTCCATGTCGCCTCTGCAT
  TCAGGATCAAACCCCGACCACCTGCCCAACCTGCTCTCCTCTTGCCACTGCCTCTTCCTC
  CCTCATTCCACCTTCCCATGCCCTGGATCCATCAGGCCACTTGATGACCCCCAACCAAGT
  GGCTCCCACACCCTGTTTTACAAAAAAGAAAAGACCAGTCCATGAGGGAGGTTTTTAAGG
  GTTTGTGGAAAATGAAAATTAGGATTTCATGATTTTTTTTTTTCAGTCCCCGTGAAGGAG
  AGCCCTTCATTTGGAGATTATGTTCTTTCGGGGAGAGGCTGAGGACTTAAAATATTCCTG
  CATTTGTGAAATGATGGTGAAAGTAAGTGGTAGCTTTTCCCTTCTTTTTCTTCTTTTTTT
  GTGATGTCCCAACTTGTAAAAATTAAAAGTTATGGTACTATGTTAGCCCCATAATTTTTT
  TTTTCCTTTTAAAACACTTCCATAATCTGGACTCCTCTGTCCAGGCACTGCTGCCCAGCC
  TCCAAGCTCCATCTCCACTCCAGATTTTTTACAGCTGCCTGCAGTACTTTACCTCCTATC
  AGAAGTTTCTCAGCTCCCAAGGCTCTGAGCAAATGTGGCTCCTGGGGGTTCTTTCTTCCT
  CTGCTGAAGGAATAAATTGCTCCTTGACATTGTAGAGCTTCTGGCACTTGGAGACTTGTA
  TGAAAGATGGCTGTGCCTCTGCCTGTCTCCCCCACCAGGCTGGGAGCTCTGCAGAGCAGG
  AAACATGACTCGTATATGTCTCAGGTCCCTGCAGGGCCAAGCACCTAGCCTCGCTCTTGG
  CAGGTACTCAGCGAATGAATGCTGTATATGTTGGGTGCAAAGTTCCCTACTTCCTGTGAC
  TTCAGCTCTGTTTTACAATAAAATCTTGAAAATGCCTAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173841 unedited
  NGTAACGTCTTGATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGAAG
  ACCTCCTGTCCTATCAGGCCCTCCCCTGGCTTTAGCTGACTTGTATGAAGAAGGAGGTGG
  AGGAGGAGGAGAAGGTGAAGACAATGCTGACTCAAAGGAGACGATCTGCCGACCCTCTGG
  GAGAAAATCCAGCAAGATGCAAGCCTTCAGAATCTGGGATGTTAACCAGAAGACCTTCTA
  TCTGAGGAACAACCAACTAGTTGCCGGATACTTGCAAGGACCAAATGTCAATTTAGAAGA
  AAAGATAGATGTGGTACCCATTGAGCCTCATGCTCTGTTCTTGGGAATCCATGGAGGGAA
  GATGTGCCTGTCCTGTGTCAAGTCTGGTGATGAGACCAGACTCCAGCTGGAGGCAGTTAA
  CATCACTGACCTGAGCGAGAACAGAAAGCAGGACAAGCGCTTCGCCTTCATCCGCTCAGA
  CAGTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTGCACAGC
  GATGGAAGCTGACCAGCCCGTCAGCCTCACCAATATGCCTGACGAAGGCGTCATGGTCAC
  CAAATTCTACTTCCAGGAGGACGAGTAGTACTGCCCAGGCCTGCCTGTTCCCATTCTTGC
  ATGGCAAGGACTGCANGGACTGCCAGTCCCCCTGCCCCAGGGCTCCCGGCTATGGNGGCA
  CTNGAGACCCAGCCATTGAGGGGTGGGACCCTCAGAAGGCGTCACAACAACCTGGTCACA
  GGACTCTGCCTNCTCTTNCACTGGACAGCCTNCATGCTGCCTNCAGAATGGGTCTTTCTA
  TGTGTGAATCAGAGCACAGCAGCCCCTGCACAAAGCCCTTNCCATGTCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_173841
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173841.1, NP_776213.1
• RefSeq Size: 1865 bp
• RefSeq ORF: 543 bp
• Locus ID: 3557
• UniProt ID: P18510
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (2) encodes the longest isoform (2).