VGCNL1 (NALCN) (NM_052867) Human Untagged Clone

CAT#: SC309141

NALCN (untagged)-Human sodium leak channel, non-selective (NALCN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VGCNL1
• Synonyms: bA430M15.1; CanIon; CLIFAHDD; IHPRF; IHPRF1; INNFD; VGCNL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_052867, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTCAAAAGGAAGCAGAGTTCCAGGGTGGAAGCCCAGCCAGTCACTGACTTTGGTCCTGATGAGTCTC
  TGTCGGATAATGCTGACATCCTCTGGATTAACAAACCATGGGTTCACTCTTTGCTGCGCATCTGTGCCAT
  CATCAGCGTCATTTCTGTTTGTATGAATACGCCAATGACCTTCGAGCACTATCCTCCACTTCAGTATGTG
  ACCTTCACTTTGGATACATTATTGATGTTTCTCTACACGGCAGAGATGATAGCAAAAATGCACATCCGGG
  GCATTGTCAAGGGGGATAGTTCCTATGTGAAAGATCGCTGGTGTGTTTTTGATGGATTTATGGTCTTTTG
  CCTTTGGGTTTCTTTGGTGCTACAGGTGTTTGAAATTGCTGATATAGTTGATCAGATGTCACCTTGGGGC
  ATGTTGCGGATTCCACGGCCACTGATTATGATCCGAGCATTCCGGATTTATTTCCGATTTGAACTGCCAA
  GGACCAGAATTACAAATATTTTAAAGCGATCGGGAGAACAAATATGGAGTGTTTCCATTTTTCTACTTTT
  CTTTCTACTTCTTTATGGAATTTTAGGAGTTCAGATGTTTGGAACATTTACTTATCACTGTGTTGTAAAT
  GACACAAAGCCAGGGAATGTAACCTGGAATAGTTTAGCTATTCCAGACACACACTGCTCACCAGAGCTAG
  AAGAAGGCTACCAGTGCCCACCTGGATTTAAATGCATGGACCTTGAAGATCTGGGACTTAGCAGGCAAGA
  GCTGGGCTACAGTGGCTTTAATGAGATAGGAACTAGTATATTCACCGTCTATGAGGCCGCCTCACAGGAA
  GGCTGGGTGTTCCTCATGTACAGAGCAATTGACAGCTTTCCCCGTTGGCGTTCCTACTTCTATTTCATCA
  CTCTCATTTTCTTCCTCGCCTGGCTTGTGAAGAACGTGTTTATTGCTGTTATCATTGAAACATTTGCAGA
  AATCAGAGTACAGTTTCAACAAATGTGGGGATCGAGAAGCAGCACTACCTCAACAGCCACCACCCAGATG
  TTTCATGAAGATGCTGCTGGAGGTTGGCAGCTGGTAGCTGTGGATGTCAACAAGCCCCAGGGACGCGCCC
  CAGCCTGCCTCCAGAAAATGATGCGGTCATCCGTTTTCCACATGTTCATCCTGAGCATGGTGACCGTGGA
  CGTGATCGTGGCGGCTAGCAACTACTACAAAGGAGAAAACTTCAGGAGGCAGTACGACGAGTTCTACCTG
  GCGGAGGTGGCTTTTACAGTACTTTTTGATTTGGAAGCACTTCTGAAGATATGGTGTTTGGGATTTACTG
  GATATATTAGCTCATCTCTCCACAAATTCGAACTACTACTCGTAATTGGAACTACTCTTCATGTATACCC
  AGATCTTTATCATTCACAATTCACGTACTTTCAGGTTCTCCGAGTAGTTCGGCTGATTAAGATTTCACCT
  GCATTAGAAGACTTTGTGTACAAGATATTTGGTCCTGGAAAAAAGCTTGGGAGTTTGGTTGTATTTACTG
  CCAGCCTCTTGATTGTTATGTCAGCAATTAGTTTGCAGATGTTCTGCTTTGTCGAAGAACTGGACAGATT
  TACTACGTTTCCGAGGGCATTTATGTCCATGTTCCAGATCCTCACCCAGGAAGGATGGGTGGACGTAATG
  GACCAAACTCTAAATGCTGTGGGACATATGTGGGCACCCGTGGTTGCCATCTATTTCATTCTCTATCATC
  TTTTTGCCACTCTGATCCTCCTGAGTTTGTTTGTTGCTGTTATTTTGGACAACTTAGAACTTGATGAAGA
  CCTAAAGAAGCTTAAACAATTAAAGCAAAGTGAAGCAAATGCGGACACCAAAGAAAAGCTCCCTTTACGC
  CTGCGAATCTTTGAAAAATTTCCAAACAGACCTCAAATGGTGAAAATCTCAAAGCTTCCTTCAGATTTTA
  CAGTTCCTAAAATCAGGGAGAGTTTTATGAAGCAGTTTATTGACCGCCAGCAACAGGACACATGTTGCCT
  CCTGAGAAGCCTCCCGACCACCTCTTCCTCCTCCTGCGACCACTCCAAACGCTCAGCAATTGAGGACAAC
  AAATACATCGACCAAAAACTTCGCAAGTCTGTTTTCAGCATCAGGGCAAGGAACCTTCTGGAAAAGGAGA
  CCGCAGTCACTAAAATCTTAAGAGCTTGCACCCGACAGCGCATGCTGAGCGGATCATTTGAGGGGCAGCC
  CGCAAAGGAGAGGTCAATCCTCAGCGTGCAGCATCATATCCGCCAAGAGCGCAGGTCACTAAGACATGGA
  TCAAACAGCCAGAGGATCAGCAGGGGAAAATCTCTTGAAACTTTGACTCAAGATCATTCCAATACAGTGA
  GATATAGAAATGCACAAAGAGAAGACAGTGAAATAAAGATGATTCAGGAAAAAAAGGAGCAAGCAGAGAT
  GAAAAGGAAAGTGCAAGAAGAGGAACTCAGAGAGAACCACCCATACTTCGATAAGCCACTGTTCATTGTC
  GGGCGAGAACACAGGTTCAGAAACTTTTGCCGGGTGGTGGTCCGAGCACGCTTCAACGCATCTAAAACAG
  ACCCTGTCACAGGAGCTGTGAAAAATACAAAGTACCATCAACTTTATGATTTGCTGGGATTGGTCACTTA
  CCTGGACTGGGTCATGATCATCGTAACCATCTGCTCTTGCATTTCCATGATGTTTGAGTCCCCGTTTCGA
  AGAGTCATGCATGCACCTACTTTGCAGATTGCTGAGTATGTGTTTGTGATATTCATGAGCATTGAGCTTA
  ATCTGAAGATTATGGCAGATGGCTTATTTTTCACTCCAACTGCTGTCATCAGGGACTTCGGTGGAGTAAT
  GGACATATTTATATATCTTGTGAGCTTGATATTTCTTTGTTGGATGCCTCAAAATGTACCTGCTGAATCG
  GGAGCTCAGCTTCTAATGGTCCTTCGGTGCCTGAGACCTCTGCGCATATTCAAACTGGTGCCCCAGATGA
  GGAAAGTTGTTCGAGAACTTTTCAGCGGCTTCAAGGAAATTTTTTTGGTCTCCATTCTTTTGCTGACATT
  AATGCTCGTTTTTGCAAGCTTTGGAGTTCAGCTTTTTGCTGGAAAACTGGCCAAGTGCAATGATCCCAAC
  ATTATTAGAAGGGAAGATTGCAATGGCATATTCAGAATTAATGTCAGTGTGTCAAAGAACTTAAATTTAA
  AATTGAGGCCTGGAGAGAAAAAACCTGGATTTTGGGTGCCCCGTGTTTGGGCGAATCCTCGGAACTTTAA
  TTTCGACAATGTGGGAAACGCTATGCTGGCGTTGTTTGAAGTTCTCTCCTTGAAAGGCTGGGTGGAAGTG
  AGAGATGTTATTATTCATCGTGTGGGGCCGATCCATGGAATCTATATTCATGTTTTTGTATTCCTGGGTT
  GCATGATTGGACTGACCCTTTTTGTTGGAGTAGTTATTGCTAATTTCAATGAAAACAAGGGGACGGCTTT
  GCTGACCGTCGATCAGAGAAGATGGGAAGACCTGAAGAGCCGACTGAAGATCGCACAGCCTCTTCATCTT
  CCGCCTCGCCCGGATAATGATGGTTTTAGAGCTAAAATGTATGACATAACCCAGCATCCATTTTTTAAGA
  GGACAATCGCATTACTCGTCCTGGCCCAGTCGGTGTTGCTCTCTGTCAAGTGGGACGTCGAGGACCCGGT
  GACCGTACCTTTGGCAACAATGTCAGTTGTTTTCACCTTCATCTTTGTTCTGGAGGTTACCATGAAGATC
  ATAGCAATGTCGCCTGCTGGCTTCTGGCAAAGCAGAAGAAACCGATACGATCTCCTGGTGACGTCGCTTG
  GCGTTGTATGGGTGGTGCTTCACTTTGCCCTCCTGAATGCATATACTTACATGATGGGCGCTTGTGTGAT
  TGTATTTAGGTTTTTCTCCATCTGTGGAAAACATGTAACGCTAAAGATGCTCCTCTTGACAGTGGTCGTC
  AGCATGTACAAGAGCTTCTTTATCATAGTAGGCATGTTTCTCTTGCTGCTGTGTTACGCTTTTGCTGGAG
  TTGTTTTATTTGGTACTGTGAAATATGGGGAGAATATTAACAGGCATGCAAATTTTTCTTCGGCTGGAAA
  AGCTATTACCGTACTGTTCCGAATTGTCACAGGTGAAGACTGGAACAAGATTATGCATGACTGTATGGTT
  CAGCCTCCGTTTTGTACTCCAGATGAATTTACATACTGGGCAACAGACTGTGGAAATTATGCTGGGGCAC
  TTATGTATTTCTGTTCATTTTATGTCATCATTGCCTACATCATGCTAAATCTGCTTGTAGCCATAATTGT
  GGAGAATTTCTCCTTGTTTTATTCCACTGAGGAGGACCAGCTTTTAAGTTACAATGATCTTCGCCACTTT
  CAAATAATATGGAACATGGTGGATGATAAAAGAGAGGGGGTGATCCCCACGTTCCGCGTCAAGTTCCTGC
  TGCGGCTACTGCGTGGGAGGCTGGAGGTGGACCTGGACAAGGACAAGCTCCTGTTTAAGCACATGTGCTA
  CGAAATGGAGAGGCTCCACAATGGCGGCGACGTCACCTTCCATGATGTCCTGAGCATGCTTTCATACCGG
  TCCGTGGACATCCGGAAGAGCTTGCAGCTGGAGGAACTCCTGGCGAGGGAGCAGCTGGAGTACACCATAG
  AGGAGGAGGTGGCCAAGCAGACCATCCGCATGTGGCTCAAGAAGTGCCTGAAGCGCATCAGAGCTAAACA
  GCAGCAGTCGTGCAGTATCATCCACAGCCTGAGAGAGAGTCAGCAGCAAGAGCTGAGCCGGTTTCTGAAC
  CCGCCCAGCATCGAGACCACCCAGCCCAGTGAGGACACGAATGCCAACAGTCAGGACAACAGCATGCAAC
  CTGAGACAAGCAGCCAGCAGCAGCTCCTGAGCCCCACGCTGTCGGATCGAGGAGGAAGTCGGCAAGATGC
  AGCCGACGCAGGGAAACCCCAGAGGAAATTTGGGCAGTGGCGTCTGCCCTCAGCCCCAAAACCAATAAGC
  CATTCAGTGTCCTCAGTCAACTTACGGTTTGGAGGAAGGACAACCATGAAATCTGTCGTGTGCAAAATGA
  ACCCCATGACTGACGCGGCTTCCTGCGGTTCTGAAGTTAAGAAGTGGTGGACCCGGCAGCTGACTGTGGA
  GAGCGACGAAAGTGGGGATGACCTTCTGGATATTTAG
  
  
• Restriction Sites: Please inquire
• ACCN: NM_052867
• Insert Size: 7000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052867.1, NP_443099.1
• RefSeq Size: 5347 bp
• RefSeq ORF: 5217 bp
• Locus ID: 259232
• UniProt ID: Q8IZF0
• Cytogenetics: 13q32.3-q33.1
• Domains: ion_trans
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a voltage-independent, nonselective cation channel which belongs to a family of voltage-gated sodium and calcium channels that regulates the resting membrane potential and excitability of neurons. This family is expressed throughout the nervous system and conducts a persistent sodium leak current that contributes to tonic neuronal excitability. The encoded protein forms a channelosome complex that includes G-protein-coupled receptors, UNC-79, UNC-80, NCA localization factor-1, and src family tyrosine kinases. Naturally occurring mutations in this gene are associated with infantile neuroaxonal dystrophy, infantile hypotonia with psychomotor retardation and characteristic facies (IHPRF) syndrome, and congenital contractures of the limbs and face with hypotonia and developmental delay (CLIFAHDD) syndrome. A knockout of the orthologous gene in mice results in paralysis with a severely disrupted respiratory rhythm, and lethality within 24 hours after birth. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 3 encode the same isoform (2).