Cytochrome P450 3A4 (CYP3A4) (NM_017460) Human Untagged Clone

CAT#: SC125488

CYP3A4 (untagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 4 (CYP3A4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 3A4
• Synonyms: CP33; CP34; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1; VDDR3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_017460 edited
  CGGCCGCGAATTCGGCACGAGAAGCTCCATGCACATAGCCCAGCAAAGAGCAACACAGAG
  CTGAAAGGAAGACTCAGAGGAGAGAGATAAGTAAGGAAAGTAGTGATGGCTCTCATCCCA
  GACTTGGCCATGGAAACCTGGCTTCTCCTGGCTGTCAGCCTGGTGCTCCTCTATCTATAT
  GGAACCCATTCACATGGACTTTTTAAGAAGCTTGGAATTCCAAGGCCCACACCTCTGCCT
  TTTTTGGGAAATATTTTGTCCTACCATAAGGGCTTTTGTATGTTTGACATGGAATGTCAT
  AAAAAGTATGGAAAAGTGTGGGGCTTTTATGATGGTCAACAGCCTGTGCTGGCTATCACA
  GATCCTGACATGATCAAAACAGTGCTAGTGAAAGAATGTTATTCTGTCTTCACAAACCGG
  AGGCCTTTTGGTCCAGTGGGATTTATGAAAAGTGCCATCTCTATAGCTGAGGATGAAGAA
  TGGAAGAGATTACGATCATTGCTGTCTCCAACCTTCACCAGTGGAAAACTCAAGGAGATG
  GTCCCTATCATTGCCCAGTATGGAGATGTGTTGGTGAGAAATCTGAGGCGGGAAGCAGAG
  ACAGGCAAGCCTGTCACCTTGAAAGACGTCTTTGGGGCCTACAGCATGGATGTGATCACT
  AGCACATCATTTGGAGTGAACATCGACTCTCTCAACAATCCACAAGACCCCTTTGTGGAA
  AACACCAAGAAGCTTTTAAGATTTGATTTTTTGGATCCATTCTTTCTCTCAATAACAGTC
  TTTCCATTCCTCATCCCAATTCTTGAAGTATTAAATATCTGTGTGTTTCCAAGAGAAGTT
  ACAAATTTTTTAAGAAAATCTGTAAAAAGGATGAAAGAAAGTCGCCTCGAAGATACACAA
  AAGCACCGAGTGGATTTCCTTCAGCTGATGATTGACTCTCAGAATTCAAAAGAAACTGAG
  TCCCACAAAGCTCTGTCCGATCTGGAGCTCGTGGCCCAATCAATTATCTTTATTTTTGCT
  GGCTATGAAACCACGAGCAGTGTTCTCTCCTTCATTATGTATGAACTGGCCACTCACCCT
  GATGTCCAGCAGAAACTGCAGGAGGAAATTGATGCAGTTTTACCCAATAAGGCACCACCC
  ACCTATGATACTGTGCTACAGATGGAGTATCTTGACATGGTGGTGAATGAAACGCTCAGA
  TTATTCCCAATTGCTATGAGACTTGAGAGGGTCTGCAAAAAAGATGTTGAGATCAATGGG
  ATGTTCATTCCCAAAGGGGTGGTGGTGATGATTCCAAGCTATGCTCTTCACCGTGACCCA
  AAGTACTGGACAGAGCCTGAGAAGTTCCTCCCTGAAAGATTCAGCAAGAAGAACAAGGAC
  AACATAGATCCTTACATATACACACCCTTTGGAAGTGGACCCAGAAACTGCATTGGCATG
  AGGTTTGCTCTCATGAACATGAAACTTGCTCTAATCAGAGTCCTTCAGAACTTCTCCTTC
  AAACCTTGTAAAGAAACACAGATCCCCCTGAAATTAAGCTTAGGAGGACTTCTTCAACCA
  GAAAAACCCGTTGTTCTAAAGGTTGAGTCAAGGGATGGCACCGTAAGTGGAGCCTGAATT
  TTCCTAAGGACTTCTGCTTTGCTCTTCAAGAAATCTGTGCCTGAGAACACCAGAGACCTC
  AAATTACTTTGTGAATAGAACTCTGAAATGAAGATGGGCTTCATCCAATGGACTGCATAA
  ATAACCGGGGATTCTGTACATGCATTGAGCTCTCTCATTGTCTGTGTAGAGTGTTATACT
  TGGGAATATAAAGGAGGTGACCAAATCAGTGTGAGGAGGTAGATTTGGCTCCTTTGCTTC
  TCACGGGACTATTTCCACCACCCCCAGTTAGCACCATTAACTCCTCCTGAGCTTTGATAA
  GAGAATCAACATTTCTCAATAATTTCCTCCCCAAATTATTAATGAAAATAAGAATTATTT
  TGATGGCTCTAACAATGACATTTATATCACATGTTTTCTCTGGAGTATTCTATAAGTTTT
  ATGTTAAATCAATAAAGACCCCTTTTCAAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAACTCG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017460 unedited
  AAGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGANAAGCTCCAT
  GCACATAGCCCAGCAAAAAGCAACACAGAGCTGAAAGGAAGACTCAGAGGAGAGAGATAA
  GTAAGGAAAGTAGTGATGGCTCTCATCCCAGACTTGGCCATGGAAACCTGGCTTCTCCTG
  GCTGTCAGCCTGGTGCTCCTCTATCTATATGGAACCCATTCACATGGACTTTTTAAGAAG
  CTTGGAATTCCAAGGCCCACACCTCTGCCTTTTTTGGGAAATATTTTGTCCTACCATAAG
  GGCTTTTGTATGTTTGACATGGAATGTCATAAAAAGTATGGAAAAGTGTGGGGCTTTTAT
  GATGGTCAACAGCCTGTGCTGGCTATCACAGATCCTGACATGATCAAAACAGTGCTAGTG
  AAAGAATGTTATTCTGTCTTCACAAACCGGAGGCCTTTTGGTCCAGTGGGATTTATGAAA
  AGTGCCATCTCTATAGCTGAGGATGAAGAATGGAAGAGATTACGATCATTGCTGTCTCCA
  ACCTTCACCAGTGGAAAACTCAAGGAGATGGTCCCTATCATTGCCCAGTATGGAGATGTG
  TTGGTGAGAAATCTGAGGCGGGAAGCAGAGACAGGCAAGCCTGTCACCTTGAAAGACGTC
  TTTGGGGCCTACAGCATGGATGTGATCACTAGCACATCATTTGGAGTGAACATCGACTCT
  CTCAACAATCCACAAGACCNCTTTGTGGAAAACACCAAGAAGCTTTTAAGATTTGATTTT
  TTGGATCCATTCTTTCTCTCATAACAGTCTTTCCATTCCTCATCCNNNATTCTGNAGTAT
  TAAATATCTGTGTGNTTTCAAGAGAAGTTACAAATTTTTTAAGAAAATCTGTAAAAGGAT
  GAAAGAAAGTCGCTCGAAGATCACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017460 unedited
  NNATTATTTTNACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTACTTTTGAAAAGGGGTCTTTATTGATTTAACATAAAACTTATAGAATACTCC
  AGAGAAAACATGTGATATAAATGTCATTGTTAGAGCCATCAAAATAATTCTTATTTTCAT
  TAATAATTTGGGGAGGAAATTATTGAGAAATGTTGATTCTCTTATCAAAGCTCAGGAGGA
  GTTAATGGTGCTAACTGGGGGTGGTGGAAATAGTCCCGTGAGAAGCAAAGGAGCCAAATC
  TACCTCCTCACACTGATTTGGTCACCTCCTTTATATTCCCAAGTATAACACTCTACACAG
  ACAATGAGAGAGCTCAATGCATGTACAGAATCCCCGGTTATTTATGCAGTCCATTGGATG
  AAGCCCATCTTCATTTCAGAGTTCTATTCACAAAGTAATTTGAGGTCTCTGGTGTTCTCA
  GGCACAGATTTCTTGAAGAGCAAAGCAGAAGTCCTTAGGAAAATTCAGGCTCCACTTACG
  GTGCCATCCCTTGACTCAACCTTTAGAACAACGGGTTTTTCTGGTTGAAGAAGTCCTCCT
  AAGCTTAATTTCAGGGGGATNCTGTGTTTCTTTACAAGGTTTGAAGGAGAAGTTCTGAAG
  GACTCTGATTAGAGCAAGTTTCATGTTCATGAGAGCAAACCTCATGCCAATGCAGTTTCT
  GGGTCCACTTCCAAGGGTGTGTATATGTAAGGATCTATNGTGNCCTTGNTCTTCTTGCTG
  AATCTTTCAGGGNAGAAACTCTCANGCTCTGTCAGTACTTTTGGTCACGGTGAAAGCATA
  NCTTGGAATCATCACACCACCCCTTTGGNAATGACATCCCCATGATCTCACATCTTTTTT
  GCAGACCTCTCAGTCTATAGCATTGGGAATATCTGACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017460
• Insert Size: 2150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017460.3, NP_059488.2
• RefSeq Size: 2768 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1576
• UniProt ID: P08684
• Cytogenetics: 7q22.1
• Domains: p450
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, P450, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.