Heparanase 1 (HPSE) (NM_006665) Human Untagged Clone

CAT#: SC111172

HPSE (untagged)-Human heparanase (HPSE), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Heparanase 1
• Synonyms: HPA; HPA1; HPR1; HPSE1; HSE1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111172 sequence for NM_006665 edited (data generated by NextGen Sequencing)
  ATGCTGCTGCGCTCGAAGCCTGCGCTGCCGCCGCCGCTGATGCTGCTGCTCCTGGGGCCG
  CTGGGTCCCCTCTCCCCTGGCGCCCTGCCCCGACCTGCGCAAGCACAGGACGTCGTGGAC
  CTGGACTTCTTCACCCAGGAGCCGCTGCACCTGGTGAGCCCCTCGTTCCTGTCCGTCACC
  ATTGACGCCAACCTGGCCACGGACCCGCGGTTCCTCATCCTCCTGGGTTCTCCAAAGCTT
  CGTACCTTGGCCAGAGGCTTGTCTCCTGCGTACCTGAGGTTTGGTGGCACCAAGACAGAC
  TTCCTAATTTTCGATCCCAAGAAGGAATCAACCTTTGAAGAGAGAAGTTACTGGCAATCT
  CAAGTCAACCAGGATATTTGCAAATATGGATCCATCCCTCCTGATGTGGAGGAGAAGTTA
  CGGTTGGAATGGCCCTACCAGGAGCAATTGCTACTCCGAGAACACTACCAGAAAAAGTTC
  AAGAACAGCACCTACTCAAGAAGCTCTGTAGATGTGCTATACACTTTTGCAAACTGCTCA
  GGACTGGACTTGATCTTTGGCCTAAATGCGTTATTAAGAACAGCAGATTTGCAGTGGAAC
  AGTTCTAATGCTCAGTTGCTCCTGGACTACTGCTCTTCCAAGGGGTATAACATTTCTTGG
  GAACTAGGCAATGAACCTAACAGTTTCCTTAAGAAGGCTGATATTTTCATCAATGGGTCG
  CAGTTAGGAGAAGATTTTATTCAATTGCATAAACTTCTAAGAAAGTCCACCTTCAAAAAT
  GCAAAACTCTATGGTCCTGATGTTGGTCAGCCTCGAAGAAAGACGGCTAAGATGCTGAAG
  AGCTTCCTGAAGGCTGGTGGAGAAGTGATTGATTCAGTTACATGGCATCACTACTATTTG
  AATGGACGGACTGCTACCAGGGAAGATTTTCTAAACCCTGATGTATTGGACATTTTTATT
  TCATCTGTGCAAAAAGTTTTCCAGGTGGTTGAGAGCACCAGGCCTGGCAAGAAGGTCTGG
  TTAGGAGAAACAAGCTCTGCATATGGAGGCGGAGCGCCCTTGCTATCCGACACCTTTGCA
  GCTGGCTTTATGTGGCTGGATAAATTGGGCCTGTCAGCCCGAATGGGAATAGAAGTGGTG
  ATGAGGCAAGTATTCTTTGGAGCAGGAAACTACCATTTAGTGGATGAAAACTTCGATCCT
  TTACCTGATTATTGGCTATCTCTTCTGTTCAAGAAATTGGTGGGCACCAAGGTGTTAATG
  GCAAGCGTGCAAGGTTCAAAGAGAAGGAAGCTTCGAGTATACCTTCATTGCACAAACACT
  GACAATCCAAGGTATAAAGAAGGAGATTTAACTCTGTATGCCATAAACCTCCATAATGTC
  ACCAAGTACTTGCGGTTACCCTATCCTTTTTCTAACAAGCAAGTGGATAAATACCTTCTA
  AGACCTTTGGGACCTCATGGATTACTTTCCAAATCTGTCCAACTCAATGGTCTAACTCTA
  AAGATGGTGGATGATCAAACCTTGCCACCTTTAATGGAAAAACCTCTCCGGCCAGGAAGT
  TCACTGGGCTTGCCAGCTTTCTCATATAGTTTTTTTGTGATAAGAAATGCCAAAGTTGCT
  GCTTGCATCTGA
  
  Clone variation with respect to NM_006665.5
  920 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006665 unedited
  TTGATATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCCAAAATGCTGCT
  GCGCTCGAAGCCTGCGCTGCCGCCGCCGCTGATGCTGCTGCTCCTGGGGCCGCTGGGTCC
  CCTCTCCCCTGGCGCCCTGCCCCGACCTGCGCAAGCACAGGACGTCGTGGACCTGGACTT
  CTTCACCCAGGAGCCGCTGCACCTGGTGAGCCCCTCGTTCCTGTCCGTCACCATTGACGC
  CAACCTGGCCACGGACCCGCGGTTCCTCATCCTCCTGGGTTCTCCAAAGCTTCGTACCTT
  GGCCAGAGGCTTGTCTCCTGCGTACCTGAGGTTTGGTGGCACCAAGACAGACTTCCTAAT
  TTTCGATCCCAAGAAGGAATCAACCTTTGAAGAGAGAAGTTACTGGCAATCTCAAGTCAA
  CCAGGATATTTGCAAATATGGATCCATCCCTCCTGATGTGGAGGAGAAGTTACGGTTGGA
  ATGGCCCTACCAGGAGCAATTGCTACTCCGAGAACACTACCAGAAAAAGTTCAAGAACAG
  CACCTACTCAAGAAGCTCTGTAGATGTGCTATACACTTTTGCAAACTGCTCAGGACTGGA
  CTTGATCTTTGGCCTAAATGCGTTATTAAGAACAGCAGATTTGCAGTGGAACAGTTCTAA
  TGCTCAGTTGCTCCTGGACTACTGCTCTTCCAAGGGGTATAACATTTCTTGGGAACTAGG
  CAATGAACCTAACAGTTTCCTTAAGAAAGCTGATATTTTCATCAATGGGTCGCAGTTAGG
  AGAAAGATTTATTCAATTGCATAAACTTCTAAGAAAGTCCACCTTCAAAATGCAAACTCT
  ATGTCCTGNATGTGGTCANCTCGAGAAAGACGCTAAGTGCTGAAGACTTCTGAAGCTGNT
  GGAGAAGTGATGATCAGTACATNCATACTACTATTGAATNACGGCTGCTACAGGAAGAAT
  CTAACCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006665 unedited
  TCTATGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  CAGGACTAGTATATTTTATTTTCAAATGCAAGCAGCAACTTTGGCATTTCTTATCACAAA
  AAAACTATATGAAAAAAGCTGGCAAGCCCAGTGAACTTCCTGGCCGGAGAGGTTTTTCCA
  TTAAAGGTGGCAAGGTTTGATCATCCACCATCTTTAAAGTTAAACCATTGAGTTGGACAG
  ATTTGGAAAGTAATCCATGAGGTCCCAAAGGTCTTAAAAGGTATTTATCCACTTGCTTGT
  TAAAAAAAGGATAGGGTAACCGCAAGTACTTGGTGACATTATGGAGGTTTATGGCATACA
  GAGTTAAATCTCCTTCTTTATACCTTGGATTGTCAGTGTTTGTGCAATGAAGGTATACTC
  GAAGCTTCCTTCTCTTTGAACCTTGCACGCTTGCCATTAACACCTTGGTGCCCACCAATT
  TCTTGAACAGAAGAGATAGCCAATAATCAGGTAAAGGATCGAAGTTTTCATCCACTAAAT
  GGTAGTTTCCTGCTCCAAAGAATACTTGCCTCATCACCACTTCTATTCCCATTCGGGCTG
  ACAGGCCCAATTTATCCAGCCACATAAAGCCAGCTGCAAAGGTGTCGGATAGCAAGGGCG
  CTCCGCCTCCATATGCAGAGCTTGTTTCTCCTAACCAGACCTTCTTGCCAGGCCCTGGTG
  CTCTCAACCACCTGNAAAAACTTTTTGCACAGATGAAAATAAAATGTCCAATACATCAGG
  GTTTAGAAAAATCTCCCTGGTAGCAGTCCGTCCATTCAAATAGTAGTGATGCCATGTAAC
  TGAATCAATCACTTCTCCACCACCTTCAGGAAGCTCTTCAGCATCTTACCGTCTTTCTTC
  GAGCTGACCAACTCAGACCATAGAGTTTGCATTTTTGAGGGGACTTCCTTAAAGTTATGC
  AATGATAAATCTCTTCTACTGCGACCATGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_006665
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006665.2, NP_006656.1
• RefSeq Size: 3726 bp
• RefSeq ORF: 1632 bp
• Locus ID: 10855
• UniProt ID: Q9Y251
• Cytogenetics: 4q21.23
• Domains: Glyco_hydro_79n
• Protein Families: Secreted Protein
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Gene Summary: Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.