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Home TrueClone PRKAA1 Clone

PRKAA1 (NM_006251) Human Kinase Deficient Mutant

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SKU Description Price Availibility*  
SC323464 PRKAA1 (untagged)-Kinase deficient mutant (K47M) of Human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1, NM_006251.4, 10ug $840 In Stock
TF81001 TurboFectin, High performance Transfection reagent (1ml/vial) $420 In Stock
TT200002 MegaTran 1.0 (0.5ml), 165 rxns for 24-well plates, 0.5ml $90 In Stock
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Click here for the corresponding wild type clone.

OriGene Data
Vector:pCMV6-XL5 Insert Size: 1700 Restriction Site:
Sequence Data: Fully Sequenced ORF          5' Read Nucleotide Sequence          Kinase domain sequence
OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: Regulation of autophagymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayHypertrophic cardiomyopathy (HCM)
Reference Data
RefSeq: NM_006251.4, NP_006242
RefSeq Size: 5085 RefSeq ORF: 5085
Synonyms : AMPK; AMPKa1
LocusID: 5562 Cytogenetic: 5p12 Domains: pkinase, TyrKc, S_TKc
Summary: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].

Transcript Variant: This variant (1) appears to represent the more predominantly expressed transcript. It lacks an in-frame coding exon compared to variant 2. The resulting isoform (1) lacks an internal segment, as compared to isoform 2.

* Delivery time is an estimate in business days. Occasional delays may occur due to unforeseen complexities in the preparation of your construct. International customers may expect an additional 1-2 weeks in shipping

 

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