ASK1 (MAP3K5) (NM_005923) Human Untagged Clone

CAT#: SC116450

MAP3K5 (untagged)-Human mitogen-activated protein kinase kinase kinase 5 (MAP3K5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASK1
• Synonyms: ASK1; MAPKKK5; MEKK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005923 edited
  ATGAGCACGGAGGCGGACGAGGGCATCACTTTCTCTGTGCCACCCTTCGCCCCCTCGGGC
  TTCTGCACCATCCCCGAGGGCGGCATCTGCAGGAGGGGAGGAGCGGCGGCGGTGGGCGAG
  GGCGAGGAGCACCAGCTGCCACCGCCGCCGCCGGGCAGCTTCTGGAACGTGGAGAGCGCC
  GCTGCCCCTGGCATCGGTTGTCCGGCGGCCACCTCCTCGAGCAGTGCCACCCGAGGCCGG
  GGCAGCTCTGTTGGCGGGGGCAGCCGACGGACCACGGTGGCATATGTGATCAACGAAGCG
  AGCCAAGGGCAACTGGTGGTGGCCGAGAGCGAGGCCCTGCAGAGCTTGCGGGAGGCGTGC
  GAGACAGTGGGCGCCACCCTGGAAACCCTGCATTTTGGGAAACTCGACTTTGGAGAAACC
  ACCGTGCTGGACCGCTTTTACAATGCAGATATTGCGGTGGTGGAGATGAGCGATGCCTTC
  CGGCAGCCGTCCTTGTTTTACCACCTTGGGGTGAGAGAAAGTTTCAGCATGGCCAACAAC
  ATCATCCTCTACTGTGATACTAACTCGGACTCTCTGCAGTCACTGAAGGAAATAATTTGC
  CAGAAGAATACTATGTGCACTGGGAACTACACCTTTGTTCCTTACATGATAACTCCACAT
  AACAAAGTCTACTGCTGTGACAGCAGCTTCATGAAGGGGTTGACAGAGCTCATGCAACCG
  AACTTCGAGCTGCTTCTTGGACCCATCTGCTTACCTCTTGTGGATCGTTTTATTCAACTT
  TTGAAGGTGGCACAAGCAAGTTCTAGCCAGTACTTCCGGGAATCTATACTCAATGACATC
  AGGAAAGCTCGTAATTTATACACTGGTAAAGAATTGGCAGCTGAGTTGGCAAGAATTCGG
  CAGCGAGTAGATAATATCGAAGTCTTGACAGCAGATATTGTCATAAATCTGTTACTTTCC
  TACAGAGATATCCAGGACTATGATTCTATTGTGAAGCTGGTAGAGACTTTAGAAAAACTG
  CCAACCTTTGATTTGGCCTCCCATCACCATGTGAAGTTTCATTATGCATTTGCACTGAAT
  AGGAGAAATCTCCCTGGTGACAGAGCAAAAGCTCTTGATATTATGATTCCCATGGTGCAA
  AGCGAAGGACAAGTTGCTTCAGATATGTATTGCCTAGTTGGTCGAATCTACAAAGATATG
  TTTTTGGACTCTAATTTCACGGACACTGAAAGCAGAGACCATGGAGCTTCTTGGTTCAAA
  AAGGCATTTGAATCTGAGCCAACACTACAGTCAGGAATTAATTATGCGGTCCTCCTCCTG
  GCAGCTGGACACCAGTTTGAATCTTCCTTTGAGCTCCGGAAAGTTGGGGTGAAGCTAAGT
  AGTCTTCTTGGTAAAAAGGGAAACTTGGAAAAACTCCAGAGCTACTGGGAAGTTGGATTT
  TTTCTGGGGGCCAGCGTCCTAGCCAATGACCACATGAGAGTCATTCAAGCATCTGAAAAG
  CTTTTTAAACTGAAGACACCAGCATGGTACCTCAAGTCTATTGTAGAGACAATTTTGATA
  TATAAGCATTTTGTGAAACTGACCACAGAACAGCCTGTGGCCAAGCAAGAACTTGTGGAC
  TTTTGGATGGATTTCCTGGTCGAGGCCACAAAGACAGATGTTACTGTGGTTAGGTTTCCA
  GTATTAATATTAGAACCAACCAAAATCTATCAACCTTCTTATTTGTCTATCAACAATGAA
  GTTGAGGAAAAGACAATCTCTATTTGGCACGTGCTTCCTGATGACAAGAAAGGTATACAT
  GAGTGGAATTTTAGTGCCTCTTCTGTCAGGGGAGTGAGTATTTCTAAATTTGAAGAAAGA
  TGCTGCTTTCTTTATGTGCTTCACAATTCTGATGATTTCCAAATCTATTTCTGTACAGAA
  CTTCATTGTAAAAAGTTTTTTGAGATGGTGAACACCATTACCGAAGAGAAGGGGAGAAGC
  ACAGAGGAAGGAGACTGTGAAAGTGACTTGCTGGAGTATGACTATGAATATGATGAAAAT
  GGTGACAGAGTCGTTTTAGGAAAAGGCACTTATGGGATAGTCTACGCAGGTCGGGACTTG
  AGCAACCAAGTCAGAATTGCTATTAAGGAAATCCCAGAGAGAGACAGCAGATACTCTCAG
  CCCCTGCATGAAGAAATAGCATTGCATAAACACCTGAAGCACAAAAATATTGTCCAGTAT
  CTGGGCTCTTTCAGTGAGAATGGTTTCATTAAAATCTTCATGGAGCAGGTCCCTGGAGGA
  AGTCTTTCTGCTCTCCTTCGTTCCAAATGGGGTCCATTAAAAGACAATGAGCAAACAATT
  GGCTTTTATACAAAGCAAATACTGGAAGGATTAAAATATCTCCATGACAATCAGATAGTT
  CACCGGGACATAAAGGGTGACAATGTGTTGATTAATACCTACAGTGGTGTTCTCAAGATC
  TCTGACTTCGGAACATCAAAGAGGCTTGCTGGCATAAACCCCTGTACTGAAACTTTTACT
  GGTACCCTCCAGTATATGGCACCAGAAATAATAGATAAAGGACCAAGAGGCTACGGAAAA
  GCAGCAGACATCTGGTCTCTGGGCTGTACAATCATTGAAATGGCCACAGGAAAACCCCCA
  TTTTATGAACTGGGAGAACCACAAGCAGCTATGTTCAAGGTGGGAATGTTTAAAGTCCAC
  CCTGAGATCCCAGAGTCCATGTCTGCAGAGGCCAAGGCATTCATACTGAAATGTTTTGAA
  CCAGATCCTGACAAGAGAGCCTGTGCTAACGACTTGCTTGTTGATGAGTTTTTAAAAGTT
  TCAAGCAAAAAGAAAAAGACACAACCTAAGCTTTCAGCTCTTTCAGCTGGATCAAATGAA
  TATCTCAGGAGTATATCCTTGCCGGTACCTGTGCTGGTGGAGGACACCAGCAGCAGCAGT
  GAGTACGGCTCAGTTTCACCCGACACGGAGTTGAAAGTGGACCCCTTCTCTTTCAAAACA
  AGAGCCAAGTCCTGCGGAGAAAGAGATGTCAAGGGAATTCGGACACTCTTTTTGGGCATT
  CCAGATGAGAATTTTGAAGATCACAGTGCTCCTCCTTCCCCTGAAGAAAAAGATTCTGGA
  TTCTTCATGCTGAGGAAGGACAGTGAGAGGCGAGCTACCCTTCACAGGATCCTGACGGAA
  GACCAAGACAAAATTGTGAGAAACCTAATGGAATCTTTAGCTCAGGGGGCTGAAGAACCG
  AAACTAAAATGGGAACACATCACAACCCTCATTGCAAGCCTCAGAGAATTTGTGAGATCC
  ACTGACCGAAAAATCATAGCCACCACACTGTCAAAGCTGAAACTGGAGCTGGACTTCGAC
  AGCCATGGCATTAGCCAAGTCCAGGTGGTACTCTTTGGTTTTCAAGATGCTGTCAATAAA
  GTTCTTCGGAATCATAACATCAAGCCGCACTGGATGTTTGCCTTAGACAGTATCATTCGG
  AAGGCGGTACAGACAGCCATTACCATCCTGGTTCCAGAACTAAGGCCACATTTCAGCCTT
  GCATCTGAGAGTGATACTGCTGATCAAGAAGACTTGGATGTAGAAGATGACCATGAGGAA
  CAGCCTTCAAATCAAACTGTCCGAAGACCTCAGGCTGTCATTGAAGATGCTGTGGCTACC
  TCAGGCGTGAGCACGCTCAGTTCTACTGTGTCTCATGATTCCCAGAGTGCTCACCGGTCA
  CTGAATGTACAGCTTGGAAGGATGAAAATAGAAACCAATAGATTACTGGAAGAATTGGTT
  CGGAAAGAGAAAGAATTACAAGCACTCCTTCATCGAGCTATTGAAGAAAAAGACCAAGAA
  ATTAAACACCTGAAGCTTAAGTCCCAACCCATAGAAATTCCTGAATTGCCTGTATTTCAT
  CTAAATTCTTCTGGCACAAATACTGAAGATTCTGAACTTACCGACTGGCTGAGAGTGAAT
  GGAGCTGATGAAGACACTATAAGCCGGTTTTTGGCTGAAGATTATACACTATTGGATGTT
  CTCTACTATGTTACACGTGATGACTTAAAATGCTTGAGACTAAGGGGAGGGATGCTGTGC
  ACACTGTGGAAGGCTATCATTGACTTTCGAAACAAACAGACTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005923 unedited
  GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTGTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGCGGCGGGT
  GGCGAGGGCGGGCTGCACCCCGAGCGCGGCGCCCTTGAGCTGCACCGCGGCGCAGGTTTG
  CGAGCCGACTTGTCAGCCGGCCAAGAAAAGGAAGCTCCGTCCCTTCCCGCTCACCCGGCT
  TCCCCACCCCTTGTACTCTAAACTCTGCAGAGGGCGAGCGGCGCGGCCACGGAGGCGCCG
  AGGAGGAGCGAGCCGCCGCCGGGCAGCGGCGTGCCCTCGGGGGAGAGGGCGCCGGAGAGG
  AGGCGGCGGCGCGGCGGCGAGGGCGCGGCGCGCGATGGCAGCTGCTTAGCCCGGCGGGCG
  CGGAGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005923 unedited
  ACCTTGGTATCNCGACCCGTCCNGCTTCNGNNANNCAANNNNAGGCCCTTTTTTTTTTAA
  CATGAGTATTTTATACTGGATTTAAAGTGCAGCGCCCTTCTCCTCTCCCTTCGATTTTCC
  CACCCCCAAGAAGACAGCTCTGTATTAATTTTTACAATTTCCATCGAATATTATATTGAG
  CACCATTCAAGTCTGTTTGTTTCGAAAGACAAAGATTTCCTTCCACTTTGNGCACAGCAN
  CCCTTCTCTTAGTCTCAAGCATTTTAAGTCATCACGTGTAACATAGTACAGAACATCCAA
  TAGTGTATAATCTTCACCCAAAAACCGGCTTATAGCGTCTTCATCACCTCCATTCACCCT
  CAGCCAGCCGGTAAGTCCACAATCCTCCCCCTTCCTCCCCCACNACTTCACATGAAACAC
  ACGCAATTCAGGAATCTCTATCGCCCGGCCCTTAACCTCCACGCGCTCAATTCCCCGGCC
  TCCCCCTTCANTANCTTTCCAACGGATTGCTCTGCCCTCTTTCTCTCCCCCAACCAATTC
  TTCCCACCATCTACCTCCCCCTACTCCCCCCCCTCCACCCCTTCCCTTCCTCTCCCCCCC
  CCCCCCTCCTCGCTACTCCTGCACCACCCTCCCATCTCCCCTCCTCCTCCCCCCCACCCT
  CCCCCTCTCTCTCCTACCCCCCTCCTTCCCCTCCTCCCCCCCCCCTCCCTTCCGCTCCCC
  CCTTACNNCCCCCCNTTCCCCCTCCCCCCCCCCTGTACCCCCTACCCCCCCCCGCCCCCC
  CCCCCTCCCTCCCCCCCTTTTTTCCCCTCCCTTTTCTCCCTCCCTTCCCCCCCCCCCCCT
  CCCCCCCTTCCCCCCCTCCTTTTCCCTCCCCCCCCTACTCTCCCTCTTATCCGCCCCCTC
  CTCCCCCCCTTCCCCTTCCCTTCCCCCCCTCCTCGCCCCCTCCCCCCCCCCCCGCACTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005923
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005923.3, NP_005914.1
• RefSeq Size: 5215 bp
• RefSeq ORF: 4125 bp
• Locus ID: 4217
• UniProt ID: Q99683
• Cytogenetics: 6q23.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]