BCAT1 (NM_005504) Human Untagged Clone

CAT#: SC116691

BCAT1 (untagged)-Human branched chain amino-acid transaminase 1, cytosolic (BCAT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCAT1
• Synonyms: BCATC; BCT1; ECA39; MECA39; PNAS121; PP18
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116691 sequence for NM_005504 edited (data generated by NextGen Sequencing)
  ATGAAGGATTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAG
  GTGGTGGGGACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAA
  AAACCAGACCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTG
  GAGTGGTCCTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCA
  TTGCACCCTGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCA
  TTTCGAGGAGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATG
  TATCGCTCTGCTGTGAGGGCAACTCTGCCGGTATTTGACAAAGAAGAGCTCTTAGAGTGT
  ATTCAACAGCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTG
  TATATTCGTCCTACATTCATTGGAACTGAGCCTTCTCTTGGAGTCAAGAAGCCTACCAAA
  GCCCTGCTCTTTGTACTCTTGAGCCCAGTGGGACCTTATTTTTCAAGTGGAACCTTTAAT
  CCAGTGTCCCTGTGGGCCAATCCCAAGTATGTAAGAGCCTGGAAAGGTGGAACTGGGGAC
  TGCAAGATGGGAGGGAATTACGGCTCATCTCTTTTTGCCCAATGTGAAGCAGTAGATAAT
  GGGTGTCAGCAGGTCCTGTGGCTCTATGGAGAGGACCATCAGATCACTGAAGTGGGAACT
  ATGAATCTTTTTCTTTACTGGATAAATGAAGATGGAGAAGAAGAACTGGCAACTCCTCCA
  CTAGATGGCATCATTCTTCCAGGAGTGACAAGGCGGTGCATTCTGGACCTGGCACATCAG
  TGGGGTGAATTTAAGGTGTCAGAGAGATACCTCACCATGGATGACTTGACAACAGCCCTG
  GAGGGGAACAGAGTGAGAGAGATGTTTGGCTCTGGTACAGCCTGTGTTGTTTGCCCAGTT
  TCTGATATACTGTACAAAGGCGAGACAATACACATTCCAACTATGGAGAATGGTCCTAAG
  CTGGCAAGCCGCATCTTGAGCAAATTAACTGATATCCAGTATGGAAGAGAAGAGAGCGAC
  TGGACAATTGTGCTATCCTGA
  
  Clone variation with respect to NM_005504.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005504 unedited
  CGCCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT
  TATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCG
  GCACGAGGGGACCGCGCTCTGCAGCCGCAGACCCGGTCCACACGGCCAGGGGCTACGACC
  CTTGGGATCTGCCCTCCGCTCAGCTCGAGCTTCCCTCGTGGCCGACGGAACAATGAAGGA
  TTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAGGTGGTGGG
  GACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAAAAACCAGA
  CCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTGGAGTGGTC
  CTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCATTGCACCC
  TGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCATTTCGAGG
  AGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATGTATCGCTC
  TGCTGTGAGGGCAACTCTGCCGGTATTTGACANAGAAGAGCTCTTAGAGTGTATTCAACA
  GCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTGTATATTCG
  TCCTACATTCATTGGAACTGAGCCTTCTCTTGGNAGTCAGAGCCTACCCAGCCCTGCTCT
  TTGTACTCTTGAGCCCAGTGGACCTTATTTTTNCAGTGGACCTTNATNCAGTGTCCCTGT
  GGCCATCCNAGTATGTAGAGCCTGGAAAGGTGNACTGNNGACTGCAGATGGGNAGGNAAT
  ACCGCTCATCTCTTTTGCCCATGTGAGCAGTAATNAATGGTGGTCACCAGGTCCTGTGGC
  TTTATGGAGAGGACATCAGATCACTGAGTGGAACTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005504 unedited
  GTCTACTATGNACCGCGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTCTTAC
  ATTTTTTCTTACAACAATCTTTTGAGAAAAGAAAGAGAATGTTTACCAGGTAGAAAAAAC
  CTGATTCCTTAACAAGACTTCTCAGTCACTTGTTTCTCTTTCCCTTTCACTTTTCCTTTG
  TGCTTTTGAAAACAAAGGCTAAAGCAGGTGTTATAAATTTATTCATTCCTGTGCTAGAGA
  GCATGGAACACATCTCTGAAGTCAACTCTTCTTTTGGTGTATCACGAGTTGAATCCTTAA
  TAAATGACTGCCACAAAGAAAATTTCTAACAGCAAATCTTTTTAGGAACTGCTCAGGTTT
  TGCCTTCGTCTTGACACTTCATTTTAAAATCTTGGTTTCTACTGATGTGTTTTCAAAAAA
  GCTGACAAACTCGGTCTGAAATGTAGACACTGAAAAAAGTGAAAGGAAGAGAACATGCAA
  TATAAGTTAATCATTATCATTTTAAGTGCCACCAGTTCTGTTTATATTAATGCACTTGTG
  AATTGCTGAATCTCATTTGATCAACCCCATTAAACATTAAATAATTGCAGCCAATTATGC
  CAATATCAGCCACAATATTAATTTGTTGATCTTTTTGAGCAGTTAGCTTCACATGTTCCT
  AAAGCTAGACCATCTTATGCCTTTAAACGATCAATTAGTGTGACAATAACCTAATCCTTG
  AAGAATGGATAATTGCCTAGTTATAATGTTGGCACTCCTGCTGTATTAAACCACTGGCAT
  TAAACCAATAGTGCACACAGAAAAAAAAAGGGCCAAAGTTCCCTGTTAAATCTTTTTAAC
  AAGAAAACGTCCAGGGCAATTTCCGGGGCTATTACCTAAAGGACAAGGTGGGATTGGGGT
  CTCCTTTTAAGGAGAACCTCCCGTTTTAAAACACTCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005504
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005504.4, NP_005495.2
• RefSeq Size: 8191 bp
• RefSeq ORF: 1161 bp
• Locus ID: 586
• UniProt ID: P54687
• Cytogenetics: 12p12.1
• Domains: aminotran_4
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.