TNFSF9 (NM_003811) Human Untagged Clone

CAT#: SC303383

TNFSF9 (untagged)-Human tumor necrosis factor (ligand) superfamily, member 9 (TNFSF9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNFSF9
• Synonyms: 4-1BB-L; CD137L; TNLG5A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003811 edited
  TCTCTCGTCATGGAATACGCCTCTGACGCTTCACTGGACCCCGAAGCCCCGTGGCCTCCC
  GCGCCCCGCGCTCGCGCCTGCCGCGTACTGCCTTGGGCCCTGGTCGCGGGGCTGCTGCTG
  CTGCTGCTGCTCGCTGCCGCCTGCGCCGTCTTCCTCGCCTGCCCCTGGGCCGTGTCCGGG
  GCTCGCGCCTCGCCCGGCTCCGCGGCCAGCCCGAGACTCCGCGAGGGTCCCGAGCTTTCG
  CCCGACGATCCCGCCGGCCTCTTGGACCTGCGGCAGGGCATGTTTGCGCAGCTGGTGGCC
  CAAAATGTTCTGCTGATCGATGGGCCCCTGAGCTGGTACAGTGACCCAGGCCTGGCAGGC
  GTGTCCCTGACGGGGGGCCTGAGCTACAAAGAGGACACGAAGGAGCTGGTGGTGGCCAAG
  GCTGGAGTCTACTATGTCTTCTTTCAACTAGAGCTGCGGCGCGTGGTGGCCGGCGAGGGC
  TCAGGCTCCGTTTCACTTGCGCTACACCTGCAGCCACTGCGCTCTGCTGCTGGGGCCGCC
  GCCCTGGCTTTGACCGTGGACCTGCCACCCGCCTCCTCCGAGGCTCGGAACTCGGCCTTC
  GGTTTCCAGGGCCGCTTGCTGCACCTGAGTGCCGGCCAGCGCCTGGGCGTCCATCTTCAC
  ACTGAGGCCAGGGCACGCCATGCCTGGCAGCTTACCCAGGGCGCCACAGTCTTGGGACTC
  TTCCGGGTGACCCCCGAAATCCCAGCCGGACTCCCTTCACCGAGGTCGGAATAACGCCCA
  GCCTGGGTGCAGCCCACCTGGACAGAGTCCGAATCCTACTCCATCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003811 unedited
  GACGACGAAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATCANATCTGGTACCGA
  GCTCGGATCCACTAGTAACGGCCGCCATGTGCTGGAATTCGCCCTTCTCTCGTCATGGAA
  TACGCCTCTGACGCTTCACTGGACCCCGAAGCCCCGTGGCCTCCCGCGCCCCGCGCTCGC
  GCCTGCCGCGTACTGCCTTGGGCCCTGGTCGCGGGGCTGCTGCTGCTGCTGCTGCTCGCT
  GCCGCCTGCGCCGTCTTCCTCGCCTGCCCCTGGGCCGTGTCCGGGGCTCGCGCCTCGCCC
  GGCTCCGCGGCCAGCCCGAGACTCCGCGAGGGTCCCGAGCTTTCGCCCGACGATCCCGCC
  GGCCTCTTGGACCTGCGGCAGGGCATGTTTGCGCAGCTGGTGGCCCAAAATGTTCTGCTG
  ATCGATGGGCCCCTGAGCTGGTACAGTGACCCAGGCCTGGCAGGCGTGTCCCTGACGGGG
  GGCCTGAGCTACAAAGAGGACACGAAGGAGCTGGTGGTGGCCAAGGCTGGAGTCTACTAT
  GTCTTCTTTCAACTAGAGCTGCGGCGCGTGGTGGCCGGCGAGGGCTCAGGCTCCGTTTCA
  CTTGCGCTACACCTGCAGCCACTGCGCTCTGCTGCTGGGGCCGCCGCCCTGGCTTTGACC
  GTGGACCTGCCACCCGCCTCCTCCGAGGCTCGGAACTCGGCCTTCGGTTTCCAGGGCCGC
  TTGCTGCACCTGAGTGCCGGCCAGCGCCTGGGCGTCCATCTTCACACTGAGGCCAGGGCA
  CGCCATGCCTGGCAGCTTACCCAGGGCGCCACAGTCTTGGGACTCTTCCGGGTGACCCCC
  GAAATCCAGCCGGACTCCCTTCACCGAGGTCGGAATAACGCCCAGCCTGGGTGCAGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003811 unedited
  ACATTGGNNGATGGCACTTNCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGATGC
  CACCCGGGATCTGTTCAGNAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGCGGCC
  GCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGGATGGAGTAGGATTCGGACTCTGTC
  CAGGTGGGCTGCACCCAGGCTGGGCGTTATTCCGACCTCGGTGAAGGGAGTCCGGCTGGG
  ATTTCGGGGGTCACCCGGAAGAGTCCCAAGACTGTGGCGCCCTGGGTAAGCTGCCAGGCA
  TGGCGTGCCCTGGCCTCAGTGTGAAGATGGACGCCCAGGCGCTGGCCGGCACTCAGGTGC
  AGCAAGCGGCCCTGGAAACCGAAGGCCGAGTTCCGAGCCTCGGAGGAGGCGGGTGGCAGG
  TCCACGGTCAAAGCCAGGGCGGCGGCCCCAGCAGCAGAGCGCAGTGGCTGCAGGTGTAGC
  GCAAGTGAAACGGAGCCTGAGCCCTCGCCGGCCACCACGCGCCGCAGCTCTAGTTGAAAG
  AAGACATAGTAGACTCCAGCCTTGGCCACCACCAGCTCCTTCGTGTCCTCTTTGTAGCTC
  AAGCCCCCCGTCAGGGACACGCCTGCCAGGCCTGGGTCACTGTACCAGCTCAGGNGCCCA
  TCGATCAGCAGAACATTTTGGGCCACCAGCTGCGCAAACATGCCCTGCCGCNNAGTCCAA
  GAGCCGGCCGGGATCGTCCGGGCGAAGCCTCGGGACCCTCGCGGAGTCTCCGGGCTGGCC
  GCGGNACCCCGCCAAAGCCCCNAGCCCCCGAACAACGGCCCAGGGGGCAGGCCGAGNAAA
  AACGGCCCAGGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_003811
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003811.2, NP_003802.1
• RefSeq Size: 1645 bp
• RefSeq ORF: 765 bp
• Locus ID: 8744
• UniProt ID: P41273
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Gene Summary: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.[provided by RefSeq, Oct 2008]