CD73 (NT5E) (NM_002526) Human Untagged Clone

CAT#: SC118577

NT5E (untagged)-Human 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD73
• Synonyms: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118577 sequence for NM_002526 edited (data generated by NextGen Sequencing)
  ATGTGTCCCCGAGCCGCGCGGGCGCCCGCGACGCTACTCCTCGCCCTGGGCGCGGTGCTG
  TGGCCTGCGGCTGGCGCCTGGGAGCTTACGATTTTGCACACCAACGACGTGCACAGCCGG
  CTGGAGCAGACCAGCGAGGACTCCAGCAAGTGCGTCAACGCCAGCCGCTGCATGGGTGGC
  GTGGCTCGGCTCTTCACCAAGGTTCAGCAGATCCGCCGCGCCGAACCCAACGTGCTGCTG
  CTGGACGCCGGCGACCAGTACCAGGGCACTATCTGGTTCACCGTGTACAAGGGCGCCGAG
  GTGGCGCACTTCATGAACGCCCTGCGCTACGATGCCATGGCACTGGGAAATCATGAATTT
  GATAATGGTGTGGAAGGACTGATCGAGCCACTCCTCAAAGAGGCCAAATTTCCAATTCTG
  AGTGCAAACATTAAAGCAAAGGGGCCACTAGCATCTCAAATATCAGGACTTTATTTGCCA
  TATAAAGTTCTTCCTGTTGGTGATGAAGTTGTGGGAATCGTTGGATACACTTCCAAAGAA
  ACCCCTTTTCTCTCAAATCCAGGGACAAATTTAGTGTTTGAAGATGAAATCACTGCATTA
  CAACCTGAAGTAGATAAGTTAAAAACTCTAAATGTGAACAAAATTATTGCACTGGGACAT
  TCGGGTTTTGAAATGGATAAACTCATCGCTCAGAAAGTGAGGGGTGTGGACGTCGTGGTG
  GGAGGACACTCCAACACATTTCTTTACACAGGCAATCCACCTTCCAAAGAGGTGCCTGCT
  GGGAAGTACCCATTCATAGTCACTTCTGATGATGGGCGGAAGGTTCCTGTAGTCCAGGCC
  TATGCTTTTGGCAAATACCTAGGCTATCTGAAGATCGAGTTTGATGAAAGAGGAAACGTC
  ATCTCTTCCCATGGAAATCCCATTCTTCTAAACAGCAGCATTCCTGAAGATCCAAGCATA
  AAAGCAGACATTAACAAATGGAGGATAAAATTGGATAATTATTCTACCCAGGAATTAGGG
  AAAACAATTGTCTATCTGGATGGCTCCTCTCAATCATGCCGCTTTAGAGAATGCAACATG
  GGCAACCTGATTTGTGATGCAATGATTAACAACAACCTGAGACACGCGGATGAAATGTTC
  TGGAACCACGTATCCATGTGCATTTTAAATGGAGGTGGTATCCGGTCGCCCATTGATGAA
  CGCAACAATGGCACAATTACCTGGGAGAACCTGGCTGCTGTATTGCCCTTTGGAGGCACA
  TTTGACCTAGTCCAGTTAAAAGGTTCCACCCTGAAGAAGGCCTTTGAGCATAGCGTGCAC
  CGCTACGGCCAGTCCACTGGAGAGTTCCTGCAGGTGGGCGGAATCCATGTGGTGTATGAT
  CTTTCCCGAAAACCTGGAGACAGAGTAGTCAAATTAGATGTTCTTTGCACCAAGTGTCGA
  GTGCCCAGTTATGACCCTCTCAAAATGGACGAGGTATATAAGGTGATCCTCCCAAACTTC
  CTGGCCAATGGTGGAGATGGGTTCCAGATGATAAAAGATGAATTATTAAGACATGACTCT
  GGTGACCAAGATATCAACGTGGTTTCTACATATATCTCCAAAATGAAAGTAATTTATCCA
  GCAGTTGAAGGTCGGATCAAGTTTTCCACAGGAAGTCACTGCCATGGAAGCTTTTCTTTA
  ATATTTCTTTCACTTTGGGCAGTGATCTTTGTTTTATACCAATAG
  
  Clone variation with respect to NM_002526.3
  1126 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002526 unedited
  NGGTCGGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCGGCTCG
  CCCGCTTTCGCACCCAGTTCACGCGCCACAGCTATGTGTCCCCGAGCCGCGCGGGCGCCC
  GCGACGCTACTCCTCGCCCTGGGCGCGGTGCTGTGGCCTGCGGCTGGCGCCTGGGAGCTT
  ACGATTTTGCACACCAACGACGTGCACAGCCGGCTGGAGCAGACCAGCGAGGACTCCAGC
  AAGTGCGTCAACGCCAGCCGCTGCATGGGTGGCGTGGCTCGGCTCTTCACCAAGGTTCAG
  CAGATCCGCCGCGCCGAACCCAACGTGCTGCTGCTGGACGCCGGCGACCAGTACCAGGGC
  ACCTATCTGGTTCACCGTGTACAAGGGCGCCGAGGTGGCGCACTTCATGAACGCCCTGCG
  CTACGATGCCATGGCACTGGGAAATCATGAATTTGATAATGGTGTGGAAGGACTGATCGA
  GCCACTCCTCAAAGAGGCCAAATTTCCAATTCTGAGTGCAAACATTAAAGCAAAGGGGCC
  ACTAGCATCTCAAATATCAGGACTTTATTTGCCATATAAAGTTCTTCCTGTTGGTGATGA
  AGTTGTGGGAATCGTTGGATACACTTCCAAAGAAACCCCTTTTCTCTCAAATCCAGGGAC
  ANATTTAGTGTTTTGAAGATGAAATCACTGCATTACAACCTGAAGTAGATAAGTTAAAAA
  CTCTAAATGTGAACAAANATATTGCACTGGGACATTCCGGGTTTTTGAAATGGATAAACT
  CATCGCTCAAAAGTGAAGGGTGTNGGACCGTCCTGTTGGGAGGGACCTTCAACACATTTT
  CTTACACAGGCAATCCCACCTCCCAAGAAGTGCCTGCTGGGTAAGACCCCATTATTAGTC
  CTTTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002526 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGCTTATCTTCAATTTTATTG
  TTGGGAATCTACAATTGCAAAGTGGTTCAAAGTCATTTCTTATACCACTTCACAATACAC
  ACACAGATTTAGGAAACTTAAAAAAAGTTCTTTAAAAAATTCTAATAGGATTTACCCTAT
  TTTACTGGCCAAGTGTAAAAAATAAACCCTAAATGTGCCTTTCTCTAATTTTCCTTGTAC
  AATCTTATTGACATTTTTTATAGCTAATCCATATATGCATAGAACCGAGGCTATTATTTT
  GCTGCAACAACTTTTATAAAAAGGTTATGAAGTTTAAAAGTATATTTTGTATCAAGAGTA
  CTTAATATTTGTAAACACTAAGAAACAAACATACTTCCTATCAAAACCTCTAGCTGCCAT
  TTGCACACTGTTTTTAAGGTGAAGCCTCCCATTTCTTGAATTACTTATGCAGAGCTGAGC
  AGGCTCATGTGGTTCCACTCCCACGTTAAGTATTTGTTCTTTGGGCATAGGTCATTTCAT
  CAGCATGGGTATTTTCTGACCACCCACTCAGAGGGTCCCACTTTCCAGCAGAAGTTCTCA
  TATCTGGGCTTAAGAATGATTTACAGTGACTGTTCAGATTTGGAGGCAGAGCGACTTAAA
  AAATGTATACCTCTTCCTAAAGTCATTTATTTTCTTCGAGAACTCTGGACATTCCATAAC
  TGGGTGTGTAGTATGAGTAGAATGAATTCAGTGCTAGCCTCTTGCTGGAGAGGACAAGTG
  CANNGTTAAATTACAGCTTATGTTAAAAGGNTCTCTTCTCATGATACCTTCATGTANAAN
  AAAGAGACAGAGGCANAGCTGATGGAATCTCATAAATAACAGCTAATGCCGTGTGTCACG
  CACTATAGCTAACAGTATCTGTTAAACAGTGTAATGCCCTTNACTCTTGCTTTCTATATT
  AAAAAGNCCTGGAGCCTGGTCTTCCCTTCTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002526
• Insert Size: 3270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002526.1, NP_002517.1
• RefSeq Size: 3547 bp
• RefSeq ORF: 1725 bp
• Locus ID: 4907
• UniProt ID: P21589
• Cytogenetics: 6q14.3
• Domains: Metallophos, 5_nucleotidaseC
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• Gene Summary: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).