MTAP (NM_002451) Human Untagged Clone

CAT#: SC118635

MTAP (untagged)-Human methylthioadenosine phosphorylase (MTAP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTAP
• Synonyms: BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118635 sequence for NM_002451 edited (data generated by NextGen Sequencing)
  ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTG
  GATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAG
  CCATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGG
  CATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGGCT
  TTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGGAG
  GAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGAGA
  CCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAATG
  GCTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGA
  CTCCGGTGCCACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGG
  GCAGAAAGCTTCATGTTCCGCACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCA
  GAGGTGGTTCTTGCTAAGGAGGCTGGAATTTGTTACGCAAGTATCGCCATGGCGACAGAT
  TATGACTGCTGGAAGGAGCACGAGGAAGCAGTTTCGGTGGACCGGGTCTTAAAGACCCTG
  AAAGAAAACGCTAATAAAGCCAAAAGCTTACTGCTCACTACCATACCTCAGATAGGGTCC
  ACAGAATGGTCAGAAACCCTCCATAACCTGAAGAATATGGCCCAGTTTTCTGTTTTATTA
  CCAAGACATTAA
  
  Clone variation with respect to NM_002451.3
  166 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002451 unedited
  ATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGCCACCGCTCTGTGGC
  TCGCTTGGTTCCCTTAGTCCCGAGCGCTCGCCCACTGCAGATTCCTTTCCCGTGCAGACA
  TGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGG
  ATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGC
  CATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGC
  ATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGGCTT
  TGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGGAGG
  AGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGAGAC
  CTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAATGG
  CTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGAC
  TCCGGTGCCACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGGG
  CAGAAAGCTTCATGTTCCGCACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCAG
  AAGTGGTTCTTGCTAAGGACGCTGTAATTTGTTACGCAAGTATCGCCATGCCGACAGATT
  ATGACTGCTGGCAAGAGCACCATGAAGCAGTTTCCGTGCACCCGGTCCTTAAGACCTTGA
  AGCAAACGCTATAAAGCCAAAAGCTACTGCTCATACCATACCTCAGATAGGTCCCAGAAT
  GGTCANAACCCTCCATACCTGGAGATATGCCCAGCTTTTGTCTTATACCAGCAATAAATG
  ACATGCTGCCCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002451 unedited
  CGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTCTAAATCCACTTTTATTTTTACAAC
  AACTTTACTATTTGATTATATTCACTGTAGAAAAATGTGAAGACACAGAAAGATTCAAAG
  AAAATAAAAATTATCCCAGTATCCCTCCCCCAAATACAATAACAAGGTTATATATATTTC
  TTTCTAGTTTTTTTGTATATTTACATAGCATTTTAAAGCAAAACTAGTACTATAAAATGC
  TACCATATCCTTTTTTCACTTCATATATAGAGCAACCGTGTCTTTTTAGGACATTGAAAT
  ATGCAAAAAGGATTTTCAGTTGGCTGATTCACAATCTAATTATAACTAAGACATAATTTA
  CCTCCAATTGCAAGGGATTCAGATTGTTTCAATTATTTATTATTATAACTATCATTATTA
  AACACCCTTCTTTATATATAATTCTTTGTATGCATCTCTGATATGGTTTTAAAGAGAAAT
  TGCTAGATGTAGAGTTCCCAGCACAAAGTACGTCCGTATTTTTAAGTCTTTAGATGCATT
  TTACCAAATTGCCCTCCAGAAATACTGTACCAATTTACAGTCGCATCAATAAATAACCAA
  AGAGTCCCATTTCTTCCTCACATTCTTTGGCAGTATAGGAAAGTAAAGATTACCCTCCAC
  CCTTTATTGTTGCAAATTTATAGGGGGAGAAGAGATTGGCGGGGTTTTTTTTCCCCTTAA
  ATGAATATTTTTTACCCATGTTACCAGCCATTGGCTTTCTTCTGATTTTGAAGTGGCCAA
  TCATTCAAGAATCTTTATACACCAATGCATGTTCTTTTACAAAATATCTTTGTAGCCAGG
  GCTTGAAGCTGATGTTTTTCCATATTTTTCAGTTAACAGAATTCCAATGATGGATTAATG
  CTCTTTTTTCCGGACACCAGCTTTTATTGCTTGCTAAAAAAAAAGTGGCCTTTTTCAGTT
  TGAAGTTCTGACTACCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002451
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002451.3, NP_002442.2
• RefSeq Size: 4937 bp
• RefSeq ORF: 852 bp
• Locus ID: 4507
• UniProt ID: Q13126
• Cytogenetics: 9p21.3
• Domains: Mtap_PNP
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]