MMP1 (NM_002421) Human Untagged Clone

CAT#: SC118645

MMP1 (untagged)-Human matrix metallopeptidase 1 (interstitial collagenase) (MMP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMP1
• Synonyms: CLG; CLGN
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118645 sequence for NM_002421 edited (data generated by NextGen Sequencing)
  ATGCACAGCTTTCCTCCACTGCTGCTGCTGCTGTTCTGGGGTGTGGTGTCTCACAGCTTC
  CCAGCGACTCTAGAAACACAAGAGCAAGATGTGGACTTAGTCCAGAAATACCTGGAAAAA
  TACTACAACCTGAAGAATGATGGGAGGCAAGTTGAAAAGCGGAGAAATAGTGGCCCAGTG
  GTTGAAAAATTGAAGCAAATGCAGGAATTCTTTGGGCTGAAAGTGACTGGGAAACCAGAT
  GCTGAAACCCTGAAGGTGATGAAGCAGCCCAGATGTGGAGTGCCTGATGTGGCTCAGTTT
  GTCCTCACTGAGGGGAACCCTCGCTGGGAGCAAACACATCTGACCTACAGGATTGAAAAT
  TACACGCCAGATTTGCCAAGAGCAGATGTGGACCATGCCATTGAGAAAGCCTTCCAACTC
  TGGAGTAATGTCACACCTCTGACATTCACCAAGGTCTCTGAGGGTCAAGCAGACATCATG
  ATATCTTTTGTCAGGGGAGATCATCGGGACAACTCTCCTTTTGATGGACCTGGAGGAAAT
  CTTGCTCATGCTTTTCAACCAGGCCCAGGTATTGGAGGGGATGCTCATTTTGATGAAGAT
  GAAAGGTGGACCAACAATTTCAGAGAGTACAACTTACATCGTGTTGCGGCTCATGAACTC
  GGCCATTCTCTTGGACTCTCCCATTCTACTGATATCGGGGCTTTGATGTACCCTAGCTAC
  ACCTTCAGTGGTGATGTTCAGCTAGCTCAGGATGACATTGATGGCATCCAAGCCATATAT
  GGACGTTCCCAAAATCCTGTCCAGCCCATCGGCCCACAAACCCCAAAAGCGTGTGACAGT
  AAGCTAACCTTTGATGCTATAACTACGATTCGGGGAGAAGTGATGTTCTTTAAAGACAGA
  TTCTACATGCGCACAAATCCCTTCTACCCGGAAGTTGAGCTCAATTTCATTTCTGTTTTC
  TGGCCACAACTGCCAAATGGGCTTGAAGCTGCTTACGAATTTGCCGACAGAGATGAAGTC
  CGGTTTTTCAAAGGGAATAAGTACTGGGCTGTTCAGGGACAGAATGTGCTACACGGATAC
  CCCAAGGACATCTACAGCTCCTTTGGCTTCCCTAGAACTGTGAAGCATATCGATGCTGCT
  CTTTCTGAGGAAAACACTGGAAAAACCTACTTCTTTGTTGCTAACAAATACTGGAGGTAT
  GATGAATATAAACGATCTATGGATCCAGGTTATCCCAAAATGATAGCACATGACTTTCCT
  GGAATTGGCCACAAAGTTGATGCAGTTTTCATGAAAGATGGATTTTTCTATTTCTTTCAT
  GGAACAAGACAATACAAATTTGATCCTAAAACGAAGAGAATTTTGACTCTCCAGAAAGCT
  AATAGCTGGTTCAACTGCAGGAAAAATTGA
  
  Clone variation with respect to NM_002421.3
  648 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002421 unedited
  CTATCACCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGCAAGAGGCTGGGAAGCCATCACTTACCTTGCACTGAGAAAGAAGA
  CAAAGGCCAGTATGCACAGCTTTCCTCCACTGCTGCTGCTGCTGTTCTGGGGTGTGGTGT
  CTCACAGCTTCCCAGCGACTCTAGAAACACAAGAGCAAGATGTGGACTTAGTCCAGAAAT
  ACCTGGAAAAATACTACAACCTGAAGAATGATGGGAGGCAAGTTGAAAAGCGGAGAAATA
  GTGGCCCAGTGGTTGAAAAATTGAAGCAAATGCAGGAATTCTTTGGGCTGAAAGTGACTG
  GGAAACCAGATGCTGAAACCCTGAAGGTGATGAAGCAGCCCAGATGTGGAGTGCCTGATG
  TGGCTCAGTTTGTCCTCACTGAGGGGAACCCTCGCTGGGAGCAAACACATCTGACCTACA
  GGATTGAAAATTACACGCCAGATTTGCCAAGAGCAGATGTGGACCATGCCATTGAGAAAG
  CCTTCCAACTCTGGAGTAATGTCACACCTCTGACATTCACCAAGGTCTCTGAGGGTCAAG
  CAGACATCATGATATCTTTTGTCAGGGGAGATCATCGGGACAACTCTCCTTTTGATGGAC
  CTGGAGGAAATCTTGCTCATGCTTTTCAACCAGGCCCAGGTATTGGAGGGGATGCTCATT
  TTGATGAAGATGAAAGGTGGACCAACAATTTCAGAGAGTACAACTTACATCGTGTTGCGG
  CTCATGAACTCGGCCATTCTCTGGNACTCTCCNATTCTACTGATATCGGGGCTTTGATGT
  ACCCTAGCTACACCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002421 unedited
  TAACCTTAGGGANCGTCGGCCGCATCCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAGTAAAAACAAGGGTGACTTTATTCCAAA
  CATAAAACACTTATTTTGGGTTAAAAAAGTTATCCCTTGCCTATCCAGGGGGACACCAGT
  GACTGCACATGTGTTCTTGAGCTGCTTTTCCTCCGGCAAAAACTCATGTCTCCTGTCTCT
  TTCTGTCTTGAAAGGATATGTTTGTCACTGAAGCTGCTCTCTGGGATCAACGTCAGAGTT
  GCATACTCTAAAAAAAATCTTTGCAACTCTGGCCTATTTGAATCCATAAGCCACAAACTT
  GACTTTTTGTACCCACCATTTGGGGAACTAAATTATATCAGTACAAAAAGGGCTACATTC
  TAAATAGTAAAAAAATATGCAAACTGAGGTATAAATAAAATTATATTCTGGGTATCAGGG
  ACTCTAGAGGTTAAAAATGACTGAGAAAATAGACAGTTCTTCAAGAAAACACCTCCTTTG
  GACTCACACCATGTGTTTTCCATTCAAATTAGTAATGTTCAATTTTTCCTGCAGTTGAAC
  CAGCTATTAGCTTTCTGGAGAGTCAAAATTCTCTTCGTTTTAGGATCAAATTTGTATTGT
  CTTGTTCCATGAAAGAATAGAAAATCCATCTTTCATGAAAACTGCATCAACTTTGTGGCC
  AATTCCAGGAAAGTCATGTGCTATCATTTTGGGATAACCCTGGATCCATAGATCGGTTAT
  ATTCATCATACCCCCAGTATTTGTTAGCAACAAAGAAGTAGGTTTTTCCAGTGTTTTCCT
  CAGAAGAGCAGCATCCATTTGCCTCACAGTTCTAGGGAACCAAAAGGAGCTGTAAATGTC
  CTTGGGGAATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002421
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002421.2, NP_002412.1
• RefSeq Size: 1973 bp
• RefSeq ORF: 1410 bp
• Locus ID: 4312
• UniProt ID: P03956
• Cytogenetics: 11q22.2
• Domains: hemopexin, Peptidase_M10, ZnMc
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Bladder cancer, Pathways in cancer, PPAR signaling pathway
• Gene Summary: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).