GFAP (NM_002055) Human Untagged Clone

CAT#: SC118873

GFAP (untagged)-Human glial fibrillary acidic protein (GFAP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GFAP
• Synonyms: ALXDRD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118873 sequence for NM_002055 edited (data generated by NextGen Sequencing)
  ATGGAGAGGAGACGCATCACCTCCGCTGCTCGCCGCTCCTACGTCTCCTCAGGGGAGATG
  ATGGTGGGGGGCCTGGCTCCTGGCCGCCGTCTGGGTCCTGGCACCCGCCTCTCCCTGGCT
  CGAATGCCCCCTCCACTCCCAACCCGGGTGGATTTCTCCCTGGCTGGGGCACTCAATGCT
  GGCTTCAAGGAGACCCGGGCCAGTGAGCGGGCAGAGATGATGGAGCTCAATGACCGCTTT
  GCCAGCTACATCGAGAAGGTTCGCTTCCTGGAACAGCAAAACAAGGCGCTGGCTGCTGAG
  CTGAACCAGCTGCGGGCCAAGGAGCCCACCAAGCTGGCAGACGTCTACCAGGCTGAGCTG
  CGAGAGCTGCGGCTGCGGCTCGATCAACTCACCGCCAACAGCGCCCGGCTGGAGGTTGAG
  AGGGACAATCTGGCACAGGACCTGGCCACTGTGAGGCAGAAGCTCCAGGATGAAACCAAC
  CTGAGGCTGGAAGCCGAGAACAACCTGGCTGCCTATAGACAGGAAGCAGATGAAGCCACC
  CTGGCCCGTCTGGATCTGGAGAGGAAGATTGAGTCGCTGGAGGAGGAGATCCGGTTCTTG
  AGGAAGATCCACGAGGAGGAGGTTCGGGAACTCCAGGAGCAGCTGGCCCGACAGCAGGTC
  CATGTGGAGCTTGACGTGGCCAAGCCAGACCTCACCGCAGCCCTGAAAGAGATCCGCACG
  CAGTATGAGGCAATGGCGTCCAGCAACATGCATGAAGCCGAAGAGTGGTACCGCTCCAAG
  TTTGCAGACCTGACAGACGCTGCTGCCCGCAACGCGGAGCTGCTCCGCCAGGCCAAGCAC
  GAAGCCAACGACTACCGGCGCCAGTTGCAGTCCTTGACCTGCGACCTGGAGTCTCTGCGC
  GGCACGAACGAGTCCCTGGAGAGGCAGATGCGCGAGCAGGAGGAGCGGCACGTGCGGGAG
  GCGGCCAGTTATCAGGAGGCGCTGGCGCGGCTGGAGGAAGAGGGGCAGAGCCTCAAGGAC
  GAGATGGCCCGCCACTTGCAGGAGTACCAGGACCTGCTCAATGTCAAGCTGGCCCTGGAC
  ATCGAGATCGCCACCTACAGGAAGCTGCTAGAGGGCGAGGAGAACCGGATCACCATTCCC
  GTGCAGACCTTCTCCAACCTGCAGATTCGAGAAACCAGCCTGGACACCAAGTCTGTGTCA
  GAAGGCCACCTCAAGAGGAACATCGTGGTGAAGACCGTGGAGATGCGGGATGGAGAGGTC
  ATTAAGGAGTCCAAGCAGGAGCACAAGGATGTGATGTGA
  
  Clone variation with respect to NM_002055.4
  141 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002055 unedited
  GCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTC
  ATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGG
  CACGAGGGCAGGATGGAGAGGAGACGCATCACCTCCGCTGCTCGCCGCTCCTACGTCTCC
  TCAGGGGAGATGATGGTGGGGGGCCTGGCTCCTGGCCGCCGTCTGGGTCCTGGCACCCGC
  CTCTCCCTGGCTCGAATGCCCCCTCCACTCCCAACCCGGGTGGATTTCTCCCTGGCTGGG
  GCACTCAATGCTGGCTTCAAGGAGACCCGGGCCAGTGAGCGGGCAGAGATGATGGAGCTC
  AATGACCGCTTTGCCAGCTACATCGAGAAGGTTCGCTTCCTGGAACAGCAAAACAAGGCG
  CTGGCTGCTGAGCTGAACCAGCTGCGGGCCAAGGAGCCCACCAAGCTGGCAGACGTCTAC
  CAGGCTGAGCTGCGAGAGCTGCGGCTGCGGCTCGATCAACTCACCGCCAACAGCGCCCGG
  CTGGAGGTTGAGAGGGACAATCTGGCACAGGACCTGGCCACTGTGAGGCAGAAGCTCCAG
  GATGAAACCAACCTGAGGCTGGAAGCCGAGAACAACCTGGCTGCCTATAGACAGGAAGCA
  GATGAAGCCACCCTGGCCCGTCTGGATCTGGAGAGGAAGATTGAGTCGCTGGAGGAGGAG
  ATCCGGTTCTTGAGGAAGATCCACGANGAGGGAGGTCGGNAACTNCAGNAGCAGCTGGCC
  CGACAGCNAGTCCATGTGGAGCTTGACGTGGCCAGCCAGACCTCACGCAGCCCTGAAAGA
  GATCGCACGCAGTATGAGGGCATGGCGTCCAGCACATGCATGAAGCCCGAGAGTGTACCG
  CTCCCAGTTGCAGACCTGACGACGCTGCTGNCCGCACGCGNAGCTGNTCCGCCAGGCCAG
  CCACGAGCCACGACTACCGNGCCAGNTGCAGTCCTTGACTGCGACCTGNNATCTCTGCNC
  GGNACAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002055 unedited
  TTGGTCGCTGCCGCAATTTANATCGAGTTTTTTTTTTTTTTTTTTGAGGGCGCAGCATTT
  GTCTTTATTTTTCCTCAGCGACTAAAGGCAGCAGCAGTGCCCTGAAGATTAGCAGCAGCA
  GCAGCAGCAGGTGGCAGGAAGGAGTGGAGGGAAAGGACACCAAGTCTTGGGGAGAGGGAA
  GGACCCTTCTTCGGCCTTAGAGGGGAGAGGAGAACCCTGAAGTGGGCCCTCCCAGTCCCA
  TCTCTGGGCACAGATCCCACCAGTCTGCTCACCAGTCTGCTCAGTCAAAGCAGAGTGGGT
  GCACTGGGGTGGACGTGTCAGCCCTGAGCACCCGGCCTCCAGGCTGCAGGAATATGAGCC
  AGTGTCTTCACTTTGCTCGTGCCTCAGTTTTACAATTGTAAAATAGGGCACTACCTAGAA
  TACTGGGTACATTTTGTGTGTGAGTAAGAAGGGACCGCAAGAGGCCCTTGGCTTAGGGAA
  AAGCAGCCGGTCACTATGGGGTGGAGAGGGTCAGACGTGTGGCAGTGGCTTACTGGGGTT
  AAGAAGCAGCAGTCCCAGGGGCTCAGAGGCTGGGGCGGCCTGGTATGACACAGCAAGGAA
  GAGGCCTTTAGAAATGGGACAAAGTCATGCCCTGCCCCCATGGATACATCCCCTTTCTCT
  CCTGTTTCAGCATCTTCAAGAGGATGAGTCACTTCCTTAATTCCCACAATCCAGAGGCCA
  AGTGCAACTGGTCACCCACAACCCCTACTTGTATGCCTAGCGCCATCCCAATTGCCTCCT
  CCTCCATCTCTACCAGCGTCCCCTCCAGTCTGCACGGGGCAGTCCTCCTGGGCTTGACCT
  CTCTGTACCCACAGCTGGGGGCCAGGCAGCCCCCCTCTATCCCTCCCAGCACTNATCCCT
  CTNCACGTACACCACAGATCCCCCCAGTGCTGAGAAATCAAGCTCCACCTGCCCACAGCG
  TGCCCACAGATGGCATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002055
• Insert Size: 3075 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002055.2, NP_002046.1
• RefSeq Size: 3035 bp
• RefSeq ORF: 1299 bp
• Locus ID: 2670
• UniProt ID: P14136
• Cytogenetics: 17q21.31
• Domains: filament, filament_head
• Protein Families: ES Cell Differentiation/IPS
• Gene Summary: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes a predominant isoform (1, also known as alpha).