Endothelin A Receptor (EDNRA) (NM_001957) Human Untagged Clone

CAT#: SC118901

EDNRA (untagged)-Human endothelin receptor type A (EDNRA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Endothelin A Receptor
• Synonyms: ET-A; ETA; ETA-R; ETAR; ETRA; hET-AR; MFDA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001957 edited
  GAATTCGGCACGAGGGTCTGACGATTGTGGAGAGGCGGTGGAGAGGCTTCATCCATCCCA
  CCCGGTCGTCGCCGGGGATTGGGGTCCCAGCGAGACCTCCCCGGGAGAAGCAGTGCCCAG
  GAGGTTTTCTGAAGCCGGGGAAGCTGTGCAGCCGAAGCCGCCGCCGCGCCGGAGCCCGGG
  ACACCGGCCACCCTCCGCGCCACCCACCCTCGCCGGCTCCGGCTTCCTCTGGCCCAGGCG
  CCGCGCGGACCCGGCAGCTGTCTGCGCACGCCGAGCTCCACGGTGAAAAAAAAGTGAAGG
  TGTAAAAGCAGCACAAGTGCAATAAGAGATATTTCCTCAAATTTGCCTCAAGATGGAAAC
  CCTTTGCCTCAGGGCATCCTTTTGGCTGGCACTGGTTGGATGTGTAATCAGTGATAATCC
  TGAGAGATACAGCACAAATCTAAGCAATCATGTGGATGATTTCACCACTTTTCGTGGCAC
  AGAGCTCAGCTTCCTGGTTACCACTCATCAACCCACTAATTTGGTCCTACCCAGCAATGG
  CTCAATGCACAACTATTGCCCACAGCAGACTAAAATTACTTCAGCTTTCAAATACATTAA
  CACTGTGATATCTTGTACTATTTTCATCGTGGGAATGGTGGGGAATGCAACTCTGCTCAG
  GATCATTTACCAGAACAAATGTATGAGGAATGGCCCCAACGCGCTGATAGCCAGTCTTGC
  CCTTGGAGACCTTATCTATGTGGTCATTGATCTCCCTATCAATGTATTTAAGCTGCTGGC
  TGGGCGCTGGCCTTTTGATCACAATGACTTTGGCGTATTTCTTTGCAAGCTGTTCCCCTT
  TTTGCAGAAGTCCTCGGTGGGGATCACCGTCCTCAACCTCTGCGCTCTTAGTGTTGACAG
  GTACAGAGCAGTTGCCTCCTGGAGTCGTGTTCAGGGAATTGGGATTCCTTTGGTAACTGC
  CATTGAAATTGTCTCCATCTGGATCCTGTCCTTTATCCTGGCCATTCCTGAAGCGATTGG
  CTTCGTCATGGTACCCTTTGAATATAGGGGTGAACAGCATAAAACCTGTATGCTCAATGC
  CACATCAAAATTCATGGAGTTCTACCAAGATGTAAAGGACTGGTGGCTCTTCGGGTTCTA
  TTTCTGTATGCCCTTGGTGTGCACTGCGATCTTCTACACCCTCATGACTTGTGAGATGTT
  GAACAGAAGGAATGGCAGCTTGAGAATTGCCCTCAGTGAACATCTTAAGCAGCGTCGAGA
  AGTGGCAAAAACAGTTTTCTGCTTGGTTGTAATTTTTGCTCTTTGCTGGTTCCCTCTTCA
  TTTAAGCCGTATATTGAAGAAAACTGTGTATAACGAGATGGACAAGAACCGATGTGAATT
  ACTTAGTTTCTTACTGCTCATGGATTACATCGGTATTAACTTGGCAACCATGAATTCATG
  TATAAACCCCATAGCTCTGTATTTTGTGAGCAAGAAATTTAAAAATTGTTTCCAGTCATG
  CCTCTGCTGCTGCTGTTACCAGTCCAAAAGTCTGATGACCTCGGTCCCCATGAACGGAAC
  AAGCATCCAGTGGAAGAACCACGATCAAAACAACCACAACACAGACCGGAGCAGCCATAA
  GGACAGCATGAACTGACCACCCTTAGAAGCACTCCTCGGTACTCCCATAATCCTCTCGGA
  GAAAAAAATCACAAGGCAACTGTGAGTCCGGGAATCTCTTCTCTGATCCTTCTTCCTTAA
  TTCACTCCCACACCCAAGAAGAAATGCTTTCCAAAACCGCAAGGGTAGACTGGTTTATCC
  ACCCACAACATCTACGAATCGTACTTCTTTAATTGATCTAATTTACATATTCTGCGTGTT
  GTATTCAGCACTAAAAAATGGTGGGAGCTGGGGGAGAATGAAGACTGTTAAATGAAACCA
  GAAGGATATTTACTACTTTTGCATGAAAATAGAGCTTTCAAGTACATGGCTAGCTTTTAT
  GGCAGTTCTGGTGAATGTTCAATGGGAACTGGTCACCATGAAACTTTAGAGATTAACGAC
  AAGATTTTCTACTTTTTTTAAGTGATTTTTTTGTCCTTCAGCCAAACACAATATGGGCTC
  AAGTCACTTTTATTTGAAATGTCATTTGGTGCCAGTATTTTTTTAACTGCATAATAGCCT
  AACATGATTATTTGAACTTATTTACACXXXXXXXXXXXXXXXXXXXXXCTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001957 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCTGACGATTGTGGAGA
  GGCGGTGGAGAGGCTTCATCCATCCCACCCGGTCGTCGCCGGGGATTGGGGTCCCAGCGA
  GACCTCCCCGGGAGAAGCAGTGCCCAGGAGGTTTTCTGAAGCCGGGGAAGCTGTGCAGCC
  GAAGCCGCCGCCGCGCCGGAGCCCGGGACACCGGCCACCCTCCGCGCCACCCACCCTCGC
  CGGCTCCGGCTTCCTCTGGCCCAGGCGCCGCGCGGACCCGGCAGCTGTCTGCGCACGCCG
  AGCTCCACGGTGAAAAAAAAGTGAAGGTGTAAAAGCAGCACAAGTGCAATAAGAGATATT
  TCCTCAAATTTGCCTCAAGATGGAAACCCTTTGCCTCAGGGCATCCTTTTGGCTGGCACT
  GGTTGGATGTGTAATCAGTGATAATCCTGAGAGATACAGCACAAATCTAAGCAATCATGT
  GGATGATTTCACCACTTTTCGTGGCACAGAGCTCAGCTTCCTGGTTACCACTCATCAACC
  CACTAATTTGGTCCTACCCAGCAATGGCTCAATGCACAACTATTGCCCACAGCAGACTAA
  AATTACTTCAGCTTTCAAATACATTAACACTGTGATATCTTGTACTATCCTCATCGTGGG
  AATGGTGGTGAATGCAACTCTGCTCAGGATCATTTACCAGAACACATGTATGAGGAATGG
  CCCCAACGCGCTGGATACCAGTCTTGCCCTTGGAGACCTTATCTATGCGGCCATTGATCT
  CCCTATCAATGTATCTAAGCTGCTGGGCTGGCCGCTGGGCCTTTTGATCACAATGACTTT
  TGCCGTATTTCTTAGCAGGCTGGTTCCCCCTTTTGCACAAGTCCTCTGTGGGGGATCACC
  GGTCCTCACCTCTGCGCTCTTAGTGTTNGACAGGGACAANACCAATTGCCTCCTGCAGTC
  GCGGTTAGGGAATTGGGATTCTTNTGTAACTGCCTCGAATCGCCTCCATTGCACCTGTTC
  TTTTCCTGGCCTTCTGAGCANGGCTNTGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001957 unedited
  NTTTAGCTCTGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGTTCTGGAAACTTTTATTTTACAC
  TTATGGGCCACTGCCAACTCAGGGCCTTGGTTTTTGGCTCATTTTTACAAAGTTACTTGT
  TGAAAAAATGTAATAAAGGTAAAAATTGGAAATTTTCCTGCTAGTAAACCACAGTTACTT
  ACCAGTCCATAAATAAAATGCCCGTTGACCCCTTCAAAAATCTATGACTTTTTGAATTTA
  TAAAAGGCATTTTTTTCATATGAAAAAAACAAAGCCTTTTGAAAAAAAAACAATTGTCTA
  AACAAAAGGGGGGGAACCTATGGCTTATATTATAGGCATATATCACTGCATATGATGCCC
  CAGGGGAATAATAAATTTGACTGCTAACACACAAAAGGGCAGTACTTTTTAACCTTGCAA
  TTCAAGCAACTGGAACCTGATGTTATTTTACCTGGCCTAAAATATGGCCCAACAAATTAT
  CTTACCTAATTTATTATCACCAATCTGTCTGGCGAAGTCTGAAAAAAATATCAAACAACC
  TGGCAATTTCATCCCCACATTGAATTGGGGATAAAAACAATTTAATTCTTCAAAGGGTAA
  TGACTTTTGCTCCACCCCAGGAAACAATTTGACCCCAAATACTGCTTTTTTAAACCAACC
  AACCAAACAAAACCTATATTGTTAGTCCACAGCTCATCTGTTTTCACCCTGCCTTTTAAT
  TCCATTGTGAATGGGACCCTGATACCAGATTTTTGAATGCCAAAGGTTGCTACACTTTAA
  AATGGCCCTGAGCCGGTTGTCTTGATGAACATCCCCTTAAATTTACTCAAACTTTGCCCT
  GTGGCACAACTGCGGNGCCCAATTTTNAATATAAATAAACCCCTCTCCTCCCCTNCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001957
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001957.1, NP_001948.1
• RefSeq Size: 4105 bp
• RefSeq ORF: 1284 bp
• Locus ID: 1909
• UniProt ID: P25101
• Cytogenetics: 4q31.22-q31.23
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• Gene Summary: This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1, also known as ET-AR) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.