CCR7 (NM_001838) Human Untagged Clone

CAT#: SC118981

CCR7 (untagged)-Human chemokine (C-C motif) receptor 7 (CCR7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCR7
• Synonyms: BLR2; CC-CKR-7; CCR-7; CD197; CDw197; CMKBR7; EBI1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118981 sequence for NM_001838 edited (data generated by NextGen Sequencing)
  ATGGACCTGGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTCTCCTTGTCATTTTCCAG
  GTATGCCTGTGTCAAGATGAGGTCACGGACGATTACATCGGAGACAACACCACAGTGGAC
  TACACTTTGTTCGAGTCTTTGTGCTCCAAGAAGGACGTGCGGAACTTTAAAGCCTGGTTC
  CTCCCTATCATGTACTCCATCATTTGTTTCGTGGGCCTACTGGGCAATGGGCTGGTCGTG
  TTGACCTATATCTATTTCAAGAGGCTCAAGACCATGACCGATACCTACCTGCTCAACCTG
  GCGGTGGCAGACATCCTCTTCCTCCTGACCCTTCCCTTCTGGGCCTACAGCGCGGCCAAG
  TCCTGGGTCTTCGGTGTCCACTTTTGCAAGCTCATCTTTGCCATCTACAAGATGAGCTTC
  TTCAGTGGCATGCTCCTACTTCTTTGCATCAGCATTGACCGCTACGTGGCCATCGTCCAG
  GCTGTCTCAGCTCACCGCCACCGTGCCCGCGTCCTTCTCATCAGCAAGCTGTCCTGTGTG
  GGCATCTGGATACTAGCCACAGTGCTCTCCATCCCAGAGCTCCTGTACAGTGACCTCCAG
  AGGAGCAGCAGTGAGCAAGCGATGCGATGCTCTCTCATCACAGAGCATGTGGAGGCCTTT
  ATCACCATCCAGGTGGCCCAGATGGTGATCGGCTTTCTGGTCCCCCTGCTGGCCATGAGC
  TTCTGTTACCTTGTCATCATCCGCACCCTGCTCCAGGCACGCAACTTTGAGCGCAACAAG
  GCCATCAAGGTGATCATCGCTGTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAAT
  GGGGTGGTCCTGGCCCAGACGGTGGCCAACTTCAACATCACCAGTAGCACCTGTGAGCTC
  AGTAAGCAACTCAACATCGTCTACGACGTCACCTACAGCCTGGCCTGCGTCCGCTGCTGC
  GTCAACCCTTTCTTGTACGCCTTCATCGGCGTCAAGTTCCGCAACGATCTCTTCAAGCTC
  TTCAAGGACCTGGGCTGCCTCAGCCAGGAGCAGCTCCGGCAGTGGTCTTCCTGTCGGCAC
  ATCCGGCGCTCCTCCATGAGTGTGGAGGCCGAGACCACCACCACCTTCTCCCCATAG
  
  Clone variation with respect to NM_001838.3
  920 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001838 unedited
  GTTAGAATTTATGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACCAGGCACAGC
  CTTCCTGTGTGGTTTTACCGCCCAGAGAGCGTCATGGACCTGGGGAAACCAATGAAAAGC
  GTGCTGGTGGTGGCTCTCCTTGTCATTTTCCAGGTATGCCTGTGTCAAGATGAGGTCACG
  GACGATTACATCGGAGACAACACCACAGTGGACTACACTTTGTTCGAGTCTTTGTGCTCC
  AAGAAGGACGTGCGGAACTTTAAAGCCTGGTTCCTCCCTATCATGTACTCCATCATTTGT
  TTCGTGGGCCTACTGGGCAATGGGCTGGTCGTGTTGACCTATATCTATTTCAAGAGGCTC
  AAGACCATGACCGATACCTACCTGCTCAACCTGGCGGTGGCAGACATCCTCTTCCTCCTG
  ACCCTTCCCTTCTGGGCCTACAGCGCGGCCAAGTCCTGGGTCTTCGGTGTCCACTTTTGC
  AAGCTCATCTTTGCCATCTACAAGATGAGCTTCTTCAGTGGCATGCTCCTACTTCTTTGC
  ATCAGCATTGACCGCTACGTGGCCATCGTCCAGGCTGTCTCAGCTCACCGCCACCGTGCC
  CGCGTCCTTCTCATCAGCAAGCTGTCCTGTGTGGGCATCTGGATACTAGCCACAGTGCTC
  TCCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAAGCGATGCGA
  TGCTCTCTCATCACAGAGCATGTGGAGGCCTTTATCACCATCCNAGTGGCCCAGATGGTG
  ATCGGCTTTCTGGTCCCCCTGCTGGCCATGAGCTTCTGNTACCTTGTCATCATCCGCACC
  CTGCTCNCAGCACGCAACTTTTGAGCGCACAAGGCCATCAGGNTGATCATCGCTGTGGTC
  GTGGTCTTCATAGTCTTNCAGCTGCCCTACATGGNNNGTGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001838 unedited
  CCCCCCAATAGTGTGNCCGCGGCCGCATTCTAGATCGAGTTTTTTTTTTTTTTTTTTAAA
  GCTGTTGTTTCCTCAAGGGAAACTTTATCTGTGTGTGGGTAAAATGTTGCTCTCTTAACG
  AATCGAAAGCAGAACATGAGGAGAGGTTTTCAGTCCCTGTGACAAAGAACAAAGAACAAG
  CTCGTGGGCCTTGGGCGGCCACTGTCACCCTCCCCGCCCCTGACATTTCCCTTGTCCTCT
  CCTCCCATCCCAGTGGAGCCAAGAGCTGAGTGCATGTCATCCCCACTCTGGAGCCCAGAG
  TGTGGCTTTGATCACGCGGAGGCAGCTGGCCTGGCCTGCAGGAAACACCACACTCTCCCC
  TGTTGAGAGCCTGGGAGGGCGACGCGGCAAGTGAGGGGATGAGTGTGCTTTTAGGGCGGC
  GTGGCAGCTGCCATTCCCGCTGGCTTGGAGGACAGTGAAGAAAACGATGGAGGGAGGGGT
  TCAGAGAGTTTGTTTGACCAGCTGATGTCCGCTTTTCCTCACCAAGCCAAGAAGTCTCCC
  CACTATCTCTGGTCTTGGAGATAAGGCCTGGTTTTCGGAAGAGCTGGTCTGAGCATTTGA
  GTCTGTGGGAGGCCAGAAGGTTCATTCAGAGGACTCTTCAGGCCACTCCCACGCCCCTTG
  CACTCACCCTCCTTGGCCCCTTCACTCCAGCAGGTGGGAACAGTTTCTGGACTTTCACTT
  TCAGTTTTTGGTTTAGGGGACAATAGCCTCTGTTTCCCAGTGTTGTCTGTCTGGTGTTAG
  CTTATCAGCCCTGTCTTTTTTTGGCATTGGTTGAGGTAGCTGGGATATGGGGTGAAGCTA
  TCTTCTGGAGCAGGGGCTTGCACTCTGAGGGGAGAGCTGCTTTTCCCTGAGCAGCTTATG
  CGGGGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001838
• Insert Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001838.2, NP_001829.1
• RefSeq Size: 2188 bp
• RefSeq ORF: 1137 bp
• Locus ID: 1236
• UniProt ID: P32248
• Cytogenetics: 17q21.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (a).