FOXO3 (NM_001455) Human Untagged Clone

CAT#: SC119227

FOXO3 (untagged)-Human forkhead box O3 (FOXO3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FOXO3
• Synonyms: AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001455 edited
  CGGAGCCTTCGCGGCGTCCACGTCCCTCCCCCGCTGCACCCCGCCCCGGCGCGAGAGGAG
  AGCGCGAGAGCCCCAGCCGCGGGCGGGCGGGCGGCGAAGATGGCAGAGGCACCGGCTTCC
  CCGGCCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCGGAGTTCGAGCCCCAGAGCCGT
  CCGCGATCCTGTACGTGGCCCCTGCAAAGGCCGGAGCTCCAAGCGAGCCCTGCCAAGCCC
  TCGGGGGAGACGGCCGCCGACTCCATGATCCCCGAGGAGGAGGACGATGAAGACGACGAG
  GACGGCGGGGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCGGCGGGAGCGGCACG
  CTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGGGTGCTGGCACCCGGAGGGCAAGAC
  CCCGGGTCTGGGCCAGCCACCGCGGCGGGCGGGCTGAGCGGGGGTACACAGGCGCTGCTG
  CAGCCTCAGCAACCGCTGCCACCGCCGCAGCCGGGGGCGGCTGGGGGCTCCGGGCAGCCG
  AGGAAATGTTCGTCGCGGCGGAACGCCTGGGGAAACCTGTCCTACGCGGACCTGATCACC
  CGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTCTGTCCCAGATCTACGAGTGGATG
  GTGCGTTGCGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCCGGCTGGAAG
  AACTCCATCCGGCACAACCTGTCACTGCATAGTCGATTCATGCGGGTCCAGAATGAGGGA
  ACTGGCAAGAGCTCTTGGTGGATCATCAACCCTGATGGGGGGAAGAGCGGAAAAGCCCCC
  CGGCGGCGGGCTGTCTCCATGGACAATAGCAACAAGTATACCAAGAGCCGTGGCCGCGCA
  GCCAAGAAGAAGGCAGCCCTGCAGACAGCCCCCGAATCAGCTGACGACAGTCCCTCCCAG
  CTCTCCAAGTGGCCTGGCAGCCCCACGTCACGCAGCAGTGATGAGCTGGATGCGTGGACG
  GACTTCCGTTCACGCACCAATTCTAACGCCAGCACAGTCAGTGGCCGCCTGTCGCCCATC
  ATGGCAAGCACAGAGTTGGATGAAGTCCAGGACGATGATGCGCCTCTCTCGCCCATGCTC
  TACAGCAGCTCAGCCAGCCTGTCACCTTCAGTAAGCAAGCCGTGCACGGTGGAACTGCCA
  CGGCTGACTGATATGGCAGGCACCATGAATCTGAATGATGGGCTGACTGAAAACCTCATG
  GACGACCTGCTGGATAACATCACGCTCCCGCCATCCCAGCCATCGCCCACTGGGGGACTC
  ATGCAGCGGAGCTCTAGCTTCCCGTATACCACCAAGGGCTCGGGCCTGGGCTCCCCAACC
  AGCTCCTTTAACAGCACGGTGTTCGGACCTTCATCTCTGAACTCCCTACGCCAGTCTCCC
  ATGCAGACCATCCAAGAGAACAAGCCAGCTACCTTCTCTTCCATGTCACACTATGGTAAC
  CAGACACTCCAGGACCTGCTCACTTCGGACTCACTTAGCCACAGCGATGTCATGATGACA
  CAGTCGGACCCCTTGATGTCTCAGGCCAGCACCGCTGTGTCTGCCCAGAATTCCCGCCGG
  AACGTGATGCTTCGCAATGATCCGATGATGTCCTTTGCTGCCCAGCCTAACCAGGGAAGT
  TTGGTCAATCAGAACTTGCTCCACCACCAGCACCAAACCCAGGGCGCTCTTGGTGGCAGC
  CGTGCCTTGTCGAATTCTGTCAGCAACATGGGCTTGAGTGAGTCCAGCAGCCTTGGGTCA
  GCCAAACACCAGCAGCAGTCTCCTGTCAGCCAGTCTATGCAAACCCTCTCGGACTCTCTC
  TCAGGCTCCTCCTTGTACTCAACTAGTGCAAACCTGCCCGTCATGGGCCATGAGAAGTTC
  CCCAGCGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAATGTGACATGGAGTCCATT
  ATCCGTAGTGAACTCATGGATGCTGATGGGTTGGATTTTAACTTTGATTCCCTCATCTCC
  ACACAGAATGTTGTTGGTTTGAACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCT
  CAGAGCTGGGTGCCAGGCTGAAGGATCACTGAGGAAGGGGAAGTGGGCAAAGCAGACCCT
  CAAACTGACACAAGACCTACAGAGAAAACCCTTTGCCAAATCTGCTCTCAGCAAGTGGAC
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001455 unedited
  CGGGGCCCCACCTCCCCCTTCCCCCCCGGTTCAGAATTGTNATACAACTCATATAGGCGG
  CCGCGNAATTCGCACGAGGGGAGTGGCGGCAGCGGGCGAGGACTCGCCGAGGACGGGGCT
  CCGGCCCGGGATAACCAACTCTCCTTCTCTCTTCTTTGGTGCTTCCCCAGGCGGCGGCGG
  CGGCGCCCGGGAGCCGGAGCCTTCGCGGCGTCCACGTCCCTCCCCCGCTGCACCCCGCCC
  CGGCGCGAGAGGAGAGCGCGAGAGCCCCAGCCGCGGGCGGGCGGGCGGCGAAGATGGCAG
  AGGCACCGGCTTCCCCGGCCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCGGAGTTCG
  AGCCCCAGAGCCGTCCGCGATCCTGTACGTGGCCCCTGCAAAGGCCGGAGCTCCAAGCGA
  GCCCTGCCAAGCCCTCGGGGGAGACGGCCGCCGACTCCATGATCCCCGAGGAGGAGGACG
  ATGAAGACGACGAGGACGGCGGNGGACGGGCCGGCTCGGCCATGGCGATCGGCGGCGGCG
  GCGGGAGCGGCACGCTGGGCTCCGGGCTGCTCCTTGAGGACTCGGCCCGGGTGCTGGCAC
  CCCGGAGGCAAGACCCCGGGTCTGGGCCAGCCACCGCNGCGGGCGGGCTGAGCGGGGTAC
  ACANGCGCTGCTGCAGCCTCAGCACCGCTGCCACCGNCGCCACCGGGGGCGGCTGGGGGC
  TTCCGGCAGCCGAGGAAAGGTTCGTCGCCGCGGAAACGCCTGGGAAAACTGTNCTACGCG
  GACCTGATCCCCCGCGCCATCCAGAGCTCCCCGGGACAACCGGTNACTCTGGCCCAAATC
  TACCAAGGGATGTGGCCGTTGCGTGCCTCAATTAAGGAATAAGGGCAACAGAAACAGATT
  TGCCGGGTGGAAGGACTCCATTCGGAACAACCTGAACTGAATTAACCAATCCTGCCGGGT
  CCCAAAGAAGGGAACTGGCAAAAGCCCTTGGGGAGACATAACCCCTGAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001455 unedited
  CTAGAGGCAGGGCGTCACAGGGAATGCCACCCGGACATCTGTGGCAGTAAAAGCTCTGAC
  CGAGGCCGCAATCTAAACCCAAGCCTCCAAAAAATCCTCCTCACTACTTCTGGAATAGCT
  CACCAGACAACGAAACCCTTTGGCGACGTTTGAAAAAGAAAGAAAAGCAGCCCTGGTGCC
  TTTACTGAAAAGAACAGGTCCGAGTTAGTAACCGGCAGACCAACACTGTTCACATTACCT
  GAGGACCCTGACAGGAAATCACAGGCCCTACTGATCTGGCACCCGTAGACTTGGCGCTGG
  GGTCCCGTCCAAAGATCACCCTGACTCAAAACCGGAAACACACTGGGCCGGACCCTGTGC
  TCCACTCCTTTTGCCTATGCACGGTTCTGGCCTGGACGGGTGCACATAGCAAACAATTGT
  GCCATTGTTCAATTTTTAGAAAAGGATAATTCCCTCCCAAACAAAAAAAGAAAGAAAATC
  CCCCCTTAGTTGATGCAATTTGTGAGCTTATGGAGTTCCATGCGCTCAAAAGCTTGTGAT
  GCTCGAACCCGGAATGGTAACTGGCACAACATTTTCTTTCTGTTTTTAAATTTGCTAGGG
  GAAGGACGGTTAACATTTTAACAGAAAAATACCGCAGTTATGCGTGTGAAAAACTCAGAA
  GCCCAAGGAAAACAAACGCCGGAACAACGCTTAAAACGTCCCATAAACCATCGCAATTTC
  AAAACACCACAGAATGGCCGACGGGGGCTCACGGTGGGCTCTGAAATGAGGTGGGTGGTG
  CCCAAGGGTCCTGTCCTCCACTGGCAGGCGGCTCACCCCCGGTCAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001455
• Insert Size: 3250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: TrueClone.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001455.2, NP_001446.1
• RefSeq Size: 3338 bp
• RefSeq ORF: 2022 bp
• Locus ID: 2309
• UniProt ID: O43524
• Cytogenetics: 6q21
• Domains: FH
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Endometrial cancer, Neurotrophin signaling pathway, Non-small cell lung cancer
• Gene Summary: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' end and represents the longer transcript compared to variant 2. Both variants encode the same protein.