Serotonin transporter (SLC6A4) (NM_001045) Human Untagged Clone

CAT#: SC119478

SLC6A4 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Serotonin transporter
• Synonyms: 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119478 sequence for NM_001045 edited (data generated by NextGen Sequencing)
  ATGGAGACGACGCCCTTGAATTCTCAGAAGCAGCTATCAGCGTGTGAAGATGGAGAAGAT
  TGTCAGGAAAACGGAGTTCTACAGAAGGTTGTTCCCACCCCAGGGGACAAAGTGGAGTCC
  GGGCAAATATCCAATGGGTACTCAGCAGTTCCAAGTCCTGGTGCGGGAGATGACACACGG
  CACTCTATCCCAGCGACCACCACCACCCTAGTGGCTGAGCTTCATCAAGGGGAACGGGAG
  ACCTGGGGCAAGAAGGTGGATTTCCTTCTCTCAGTGATTGGCTATGCTGTGGACCTGGGC
  AATGTCTGGCGCTTCCCCTACATATGTTACCAGAATGGAGGGGGGGCATTCCTCCTCCCC
  TACACCATCATGGCCATTTTTGGGGGAATCCCGCTCTTTTACATGGAGCTCGCACTGGGA
  CAGTACCACCGAAATGGATGCATTTCAATATGGAGGAAAATCTGCCCGATTTTCAAAGGG
  ATTGGTTATGCCATCTGCATCATTGCCTTTTACATTGCTTCCTACTACAACACCATCATG
  GCCTGGGCGCTATACTACCTCATCTCCTCCTTCACGGACCAGCTGCCCTGGACCAGCTGC
  AAGAACTCCTGGAACACTGGCAACTGCACCAATTACTTCTCCGAGGACAACATCACCTGG
  ACCCTCCATTCCACGTCCCCTGCTGAAGAATTTTACACGCGCCACGTCCTGCAGATCCAC
  CGGTCTAAGGGGCTCCAGGACCTGGGGGGCATCAGCTGGCAGCTGGCCCTCTGCATCATG
  CTGATCTTCACTGTTATCTACTTCAGCATCTGGAAAGGCGTCAAGACCTCTGGCAAGGTG
  GTGTGGGTGACAGCCACCTTCCCTTATATCATCCTTTCTGTCCTGCTGGTGAGGGGTGCC
  ACCCTCCCTGGAGCCTGGAGGGGTGTTCTCTTCTACTTGAAACCCAATTGGCAGAAACTC
  CTGGAGACAGGGGTGTGGATAGATGCAGCCGCTCAGATCTTCTTCTCTCTTGGTCCGGGC
  TTTGGGGTCCTGCTGGCTTTTGCTAGCTACAACAAGTTCAACAACAACTGCTACCAAGAT
  GCCCTGGTGACCAGCGTGGTGAACTGCATGACGAGCTTCGTTTCGGGATTTGTCATCTTC
  ACAGTGCTCGGTTACATGGCTGAGATGAGGAATGAAGATGTGTCTGAGGTGGCCAAAGAC
  GCAGGTCCCAGCCTCCTCTTCATCACGTATGCAGAAGCGATAGCCAACATGCCAGCGTCC
  ACTTTCTTTGCCATCATCTTCTTTCTGATGTTAATCACGCTGGGCTTGGACAGCACGTTT
  GCAGGCTTGGAGGGGGTGATCACGGCTGTGCTGGATGAGTTCCCACACGTCTGGGCCAAG
  CGCCGGGAGCGGTTCGTGCTCGCCGTGGTCATCACCTGCTTCTTTGGATCCCTGGTCACC
  CTGACTTTTGGAGGGGCCTACGTGGTGAAGCTGCTGGAGGAGTATGCCACGGGGCCCGCA
  GTGCTCACTGTCGCGCTGATCGAAGCAGTCGCTGTGTCTTGGTTCTATGGCATCACTCAG
  TTCTGCAGGGACGTGAAGGAAATGCTCGGCTTCAGCCCGGGGTGGTTCTGGAGGATCTGC
  TGGGTGGCCATCAGCCCTCTGTTTCTCCTGTTCATCATTTGCAGTTTTCTGATGAGCCCG
  CCACAACTACGACTTTTCCAATATAATTATCCTTACTGGAGTATCATCTTGGGTTACTGC
  ATAGGAACCTCATCTTTCATTTGCATCCCCACATATATAGCTTATCGGTTGATCATCACT
  CCAGGGACATTTAAAGAGCGTATTATTAAAAGTATTACCCCAGAAACACCAACAGAAATT
  CCTTGTGGGGACATCCGCTTGAATGCTGTGTAA
  
  Clone variation with respect to NM_001045.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001045 unedited
  GCGTCAGCATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGTCCCTCCC
  CTCCTGGCTCTGGGGTCGGGCGCGCACCCCGCCCCGTAGCGCGGCCCCTCCCTGGCGAGC
  GCAACCCCATCCAGCGGGAGCGCGGAGCCGCGGCCGCGGGGAAGCATTAAGTTTATTCGC
  CTCAAAGTGACGCAAAAATTCTTCAAGAGCTCTTTGGCGGCGGCTATCTAGAGATCAGAC
  CATGTGAGGGCCCGCGGGTACAAATACGGCCGCGCCGGCGCCCCTCCGCACAGCCAGCGC
  CGCCGGGTGCCTCGAGGGCGCGAGGCCAGCCCGCCTGCCCAGCCCGGGACCAGCCTCCCC
  GCGCAGCCTGGCAGAGTCAATCCCGACGTGTCAATCCCGACGATAGAGAGCTCGGAGGTG
  ATCCACAAATCCAAGCACCCAGAGATCAATTGGGATCCTTGGCAGATGGACATCAGTGTC
  ATTTACTAACCAGCAGGATGGAGACGACGCCCTTGAATTCTCAGAAGCAGCTATCAGCGT
  GTGAAGATGGAGAAGATTGTCAGGAAAACGGAGTTCTACAGAAGGTTGTTCCCACCCCAG
  GGGACAAAGTGGAGTCCGGGCAAATATCCAATGGGTACTCAGCAGTTCCAAGTCCTGGTG
  CGGGAGATGACACACGGCACTCTATCCCAGCGACCACCACCACCCTAGTGGCTGAGCTTC
  ATCAGGGGGAACGGGAGACCTGGGGCAAGAAGGTGGGATTTCCTCTCTCAGTGATGGGCT
  ATGCTGTGGACCTGGCAATGTCTGGCGCTTCCCCTACTATGTTACCAGAATGGAGGGNGG
  GGCATTCTNCTNCCCTAAACATCATGGGCATTTTTGGGGAAATCCGCTCTTTTACTGGAG
  CTCGCACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001045 unedited
  AAATACTATGGATCGCTGTCCGCATCTCTAGTTGTTTTTTTTTTTTTTTTTTTTGAGTTG
  GTAGAATTTGTTACTGTAAGAAAAATTGGGGAATTCATATATTTGTAATGGGCCCAAAAT
  ATTGGACTAGAGATAAGAATACACTGGAAACAACAATATACACAGACTCACAAGCTTGCA
  TGGACACACTATTTTTCATTTTAGCTTCTTACATCTTCCTTTCCTGATGCCACAGCAGCA
  CATGGATTAGAAGGTTCTAGTAGATTCCAGCAATAAAATTCTATATGCTACTCAGAAAAT
  ATATGGCTAGCGAGATAGCATCCCTGTTCTCTCCTACGCAGTTTACGTATTCATGAACAG
  TGAAACCTTTAGAAGCAAACAGATATCAGATACCAATGATCATACCAAGAAATGATACAG
  GAGACTTTCACAGACTTGATTCTAAACCTAATCCTAACTGATGATAGGGTGGTTTTCATC
  ACCTCCATCCACATCCTCACACGTCCAAAATAGGCCAGCTCAGCTGTGTCACAGTCTACC
  ATGGGAATATGTCCAGGGGAATCCATGGAAATAAGTGGCTGGAGGCCTTGAGTCTGGGCA
  CCAGACTGTGTCCCTGTGGAGAATGCCCTTCCATCATCAGGCTTAGCTGGAAACTCATTC
  ACTTGGGAGGGGAGAAGGCANGCGTGCCTCATCAGAACTGGGNAGAGANGNTGTGGANAA
  ACCTTTTTCCTCTCGGTGAGTGTGTTACACAGCATTCAAGCGGATGTCCCCACAAAGAAT
  TTCTGGTGGTGGTTTCTGGGGTAATACCTTNTAATAANTACGCTCTTTAAATGTCCCTGG
  AGTGATGATCAACCGATNAGCTATATATGTGGGGGATGCAAATGAAAGATGAGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001045
• Insert Size: 3100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001045.2, NP_001036.1
• RefSeq Size: 2756 bp
• RefSeq ORF: 1893 bp
• Locus ID: 6532
• UniProt ID: P31645
• Cytogenetics: 17q11.2
• Domains: SNF, 5HT_transporter
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake. There have been conflicting results in the literature about the possible effect, if any, that this polymorphism may play in behavior and depression. [provided by RefSeq, May 2019]