Angiotensin II Type 1 Receptor (AGTR1) (NM_000685) Human Untagged Clone

CAT#: SC108918

AGTR1 (untagged)-Human angiotensin II receptor, type 1 (AGTR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Angiotensin II Type 1 Receptor
• Synonyms: AG2S; AGTR1B; AT1; AT1AR; AT1B; AT1BR; AT1R; AT2R1; HAT1R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000685 edited
  TGGGTTTTTATCTGAATAACTCACTGATGCCATCCCAGAAAGTCGGCACCAGGTGTATTT
  GATATAGTGTTTGCAACAAATTCGACCCAGGTGATCAAAATGATTCTCAACTCTTCTACT
  GAAGATGGTATTAAAAGAATCCAAGATGATTGTCCCAAAGCTGGAAGGCATAATTACATA
  TTTGTCATGATTCCTACTTTATACAGTATCATCTTTGTGGTGGGAATATTTGGAAACAGC
  TTGGTGGTGATAGTCATTTACTTTTATATGAAGCTGAAGACTGTGGCCAGTGTTTTTCTT
  TTGAATTTAGCACTGGCTGACTTATGCTTTTTACTGACTTTGCCACTATGGGCTGTCTAC
  ACAGCTATGGAATACCGCTGGCCCTTTGGCAATTACCTATGTAAGATTGCTTCAGCCAGC
  GTCAGTTTCAACCTGTACGCTAGTGTGTTTCTACTCACGTGTCTCAGCATTGATCGATAC
  CTGGCTATTGTTCACCCAATGAAGTCCCGCCTTCGACGCACAATGCTTGTAGCCAAAGTC
  ACCTGCATCATCATTTGGCTGCTGGCAGGCTTGGCCAGTTTGCCAGCTATAATCCATCGA
  AATGTATTTTTCATTGAGAACACCAATATTACAGTTTGTGCTTTCCATTATGAGTCCCAA
  AATTCAACCCTTCCGATAGGGCTGGGCCTGACCAAAAATATACTGGGTTTCCTGTTTCCT
  TTTCTGATCATTCTTACAAGTTATACTCTTATTTGGAAGGCCCTAAAGAAGGCTTATGAA
  ATTCAGAAGAACAAACCAAGAAATGATGATATTTTTAAGATAATTATGGCAATTGTGCTT
  TTCTTTTTCTTTTCCTGGATTCCCCACCAAATATTCACTTTTCTGGATGTATTGATTCAA
  CTAGGCATCATACGTGACTGTAGAATTGCAGATATTGTGGACACGGCCATGCCTATCACC
  ATTTGTATAGCTTATTTTAACAATTGCCTGAATCCTCTTTTTTATGGCTTTCTGGGGAAA
  AAATTTAAAAGATATTTTCTCCAGCTTCTAAAATATATTCCCCCAAAAGCCAAATCCCAC
  TCAAACCTTTCAACAAAAATGAGCACGCTTTCCTACCGCCCCTCAGATAATGTAAGCTCA
  TCCACCAAGAAGCCTGCACCATGTTTTGAGGTTGAGTGACATGTTCGAAACCTGTCCATA
  AAGTAATTTTGTGAAAGAAGGAGCAAGAGAACATTCCTCTGCAGCACTTCACTACCAAAT
  GAGCATTAGCTACTTTTCATGGGTTTTTATCTGAATAACTCACTGATGCCATCCCAGAAA
  GTCGGCACCAGGTGTATTTGATATAGTGTTTGCAACAAATTCGACCCAGGTGATCAAAAT
  GATTCTCAACTCTTCTACTGAAGATGGTATTAAAAGAATCCAAGATGATTGTCCCAAAGC
  TGGAAGGCATAATTACATATTTGTCATGATTCCTACTTTATACAGTATCATCTTTGTGGT
  GGGAATATTTGGAAACAGCTTGGTGGTGATAGTCATTTACTTTTATATGAAGCTGAAGAC
  TGTGGCCAGTGTTTTTCTTTTGAATTTAGCACTGGCTGACTTATGCTTTTTACTGACTTT
  GCCACTATGGGCTGTCTACACAGCTATGGAATACCGCTGGCCCTTTGGCAATTACCTATG
  TAAGATTGCTTCAGCCAGCGTCAGTTTCAACCTGTACGCTAGTGTGTTTCTACTCACGTG
  TCTCAGCATTGATCGATACCTGGCTATTGTTCACCCAATGAAGTCCCGCCTTCGACGCAC
  AATGCTTGTAGCCAAAGTCACCTGCATCATCATTTGGCTGCTGGCAGGCTTGGCCAGTTT
  GCCAGCTATAATCCATCGAAATGTATTTTTCATTGAGAACACCAATATTACAGTTTGTGC
  TTTCCATTATGAGTCCCAAAATTCAACCCTTCCGATAGGGCTGGGCCTGACCAAAAATAT
  ACTGGGTTTCCTGTTTCCTTTTCTGATCATTCTTACAAGTTATACTCTTATTTGGAAGGC
  CCTAAAGAAGGCTTATGAAATTCAGAAGAACAAACCAAGAAATGATGATATTTTTAAGAT
  AATTATGGCAATTGTGCTTTTCTTTTTCTTTTCCTGGATTCCCCACCAAATATTCACTTT
  TCTGGATGTATTGATTCAACTAGGCATCATACGTGACTGTAGAATTGCAGATATTGTGGA
  CACGGCCATGCCTATCACCATTTGTATAGCTTATTTTAACAATTGCCTGAATCCTCTTTT
  TTATGGCTTTCTGGGGAAAAAATTTAAAAGATATTTTCTCCAGCTTCTAAAATATATTCC
  CCCAAAAGCCAAATCCCACTCAAACCTTTCAACAAAAATGAGCACGCTTTCCTACCGCCC
  CTCAGATAATGTAAGCTCATCCACCAAGAAGCCTGCACCATGTTTTGAGGTTGAGTGACA
  TGTTCGAAACCTGTCCATAAAGTAATTTTGTGAAAGAAGGAGCAAGAGAACATTCCTCTG
  CAGCACTTCACTACCAAATGAGCATTAGCTACTTTTCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000685 unedited
  NNNTTGTCGGATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGCAGGAC
  CCCAGGCAGCAGCGAGTGACAGGACGTCTGGACCGGCGCGCCGCTAGCAGCTCTGCCGGG
  CCGCGGCGGTGATCGATGGGGAGCGGCTGGAGCGGACCCAGCGAGTGAGGGCGCACAGCC
  GGGACGCCGAGGCGGCGGGCGGGAGACCCGCACCAGCGCAGCCGGCCCTCGGCGGGACGT
  GACGCAGCGCCCGGGGCGCGGGTTTGATATTTGACAAATTGATCTAAAATGGCTGGGTTT
  TTATCTGAATAACTCACTGATGCCATCCCAGAAAGTCGGCACCAGGTGTATTTGATATAG
  TGTTTGCAACAAATTCGACCCAGGTGATCAAAATGATTCTCAACTCTTCTACTGAAGATG
  GTATTAAAAGAATCCAAGATGATTGTCCCAAAGCTGGAAGGCATAATTACATATTTGTCA
  TGATTCCTACTTTATACAGTATCATCTTTGTGGTGGGAATATTTGGAAACAGCTTGGTGG
  TGATAGTCATTTACTTTTATATGAAGCTGAAGACTGTGGCCAGTGTTTTTCTTTTGAATT
  TAGCACTGGCTGACTTATGCTTTTTACTGACTTTGCCACTTATGGGCTGTCTACACAGCT
  ATGGAATACCGCTGGCCCTTTGGCAATTACCTATGTAAGATTGCTTCAGCCAGCGTCAGT
  TTCAACCTGTACGCTAGTGTGTTTCTACTCACGTGTCTCAGCATTGATCGATACCTGGCT
  ATTGNTCACCCAATGAAGTCCCGCCTTCGACGCACAATGCTTGTAGCCAAAGTCACCTGC
  ATCATCATTTGGCTGCTGGNCAGCTTGGCCAGTTTGCCGCTATTATCCATCGAATGTATT
  TTTCATTGAGACACATATTACAGTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000685 unedited
  NTTTCTTTCAAAATTTTTTTTTTAAGTAAATTAACTTTGCCAGATTTTAATCAATTAACA
  GCAGTTTAGAGATATAGATATACATATATATATATGTGTAGAATATATACTTTTTTTAAA
  CAGGAAGGCATACTTTATGATATATAATCTTTTTACCACAAACTATATACACTTCTCAGA
  TATTAAATCAAACTAATATACTAGGAAGACACTACTACTTGGGACCAGTGCAGCACCTTT
  ACAAGTAGTTTAACTTTTATATGTGACTTTAGTAACACCATTATACAAATAAGCCATCTT
  ACGGGCATTGTTTTGGCAGTGTAAACCTATAAGACACAGGTTGAATTTCACAAAAAGATC
  TGCAACTTGACGACTACTGCTTAGCATATCTTTGTACAAAATGTGCAGTACCAGGTGCAA
  GTGTAGCACAGTTGCTAATAGCTGAAAACCGGCACGAAAACTTTACTGCCCTTTGGAAAC
  TGGACAGAACAATCTGGAACTCTCATCTCCTGTTGCTCCTCTAACGATTTAATATATTCT
  AAATACCTTTATGTGGAAATAAAAAATAACAGGACAAAAGCAGGCTAGGGAGATTGCATT
  TCTGTCAGTAAAATTTCTCAAATCAACACATTCATCGAGTTTCTGACATTGTTCTTCGAG
  CAGCCGTCATCTGTCTAATGCAAAATGTGGCTTTGCTTTGTCTTGTTGCAAAAGGAAAGA
  AAAGCTTTTGTTCAGAGCTTTAGAAAAGTCGGTTCAGTCCACATAATGCATTTTCTCCTT
  CAATTCTNGAAAGTAGCTAATGCTCATTTGGTAGTGAAGTGCTGCAGAGGAATGTTCTCT
  TGCTCCTTCTTTCACAAATACTNTATGGACAGGNTNNCGACATGTCACTCAACCTCAAAC
  AT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000685
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000685.3, NP_000676.1
• RefSeq Size: 2347 bp
• RefSeq ORF: 1080 bp
• Locus ID: 185
• UniProt ID: P30556
• Cytogenetics: 3q24
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Renin-angiotensin system, Vascular smooth muscle contraction
• Gene Summary: Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1), also known as pC2, hAT1R-B (PubMed ID:16504375) or hAT1R-C (PubMed ID:11158334), encodes the predominant isoform (1). Variants 1 and 2 encode the same isoform 1.