LDL Receptor (LDLR) (NM_000527) Human Untagged Clone

CAT#: SC110941

LDLR (untagged)-Human low density lipoprotein receptor (LDLR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LDL Receptor
• Synonyms: FH; FHC; FHCL1; LDLCQ2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000527 edited
  GAATTCGGCACCAGGGACACAGCAGGTCGTGATCCGGGTCGGGACACTGCCTGGCAGAGG
  CTGCGAGCATGGGGCCCTGGGGCTGGAAATTGCGCTGGACCGTCGCCTTGCTCCTCGCCG
  CGGCGGGGACTGCAGTGGGCGACAGATGCGAAAGAAACGAGTTCCAGTGCCAAGACGGGA
  AATGCATCTCCTACAAGTGGGTCTGCGATGGCAGCGCTGAGTGCCAGGATGGCTCTGATG
  AGTCCCAGGAGACGTGCTTGTCTGTCACCTGCAAATCCGGGGACTTCAGCTGTGGGGGCC
  GTGTCAACCGCTGCATTCCTCAGTTCTGGAGGTGCGATGGCCAAGTGGACTGCGACAACG
  GCTCAGACGAGCAAGGCTGTCCCCCCAAGACGTGCTCCCAGGACGAGTTTCGCTGCCACG
  ATGGGAAGTGCATCTCTCGGCAGTTCGTCTGTGACTCAGACCGGGACTGCTTGGACGGCT
  CAGACGAGGCCTCCTGCCCGGTGCTCACCTGTGGTCCCGCCAGCTTCCAGTGCAACAGCT
  CCACCTGCATCCCCCAGCTGTGGGCCTGCGACAACGACCCCGACTGCGAAGATGGCTCGG
  ATGAGTGGCCGCAGCGCTGTAGGGGTCTTTACGTGTTCCAAGGGGACAGTAGCCCCTGCT
  CGGCCTTCGAGTTCCACTGCCTAAGTGGCGAGTGCATCCACTCCAGCTGGCGCTGTGATG
  GTGGCCCCGACTGCAAGGACAAATCTGACGAGGAAAACTGCGCTGTGGCCACCTGTCGCC
  CTGACGAATTCCAGTGCTCTGATGGAAACTGCATCCATGGCAGCCGGCAGTGTGACCGGG
  AATATGACTGCAAGGACATGAGCGATGAAGTTGGCTGCGTTAATGTGACACTCTGCGAGG
  GACCCAACAAGTTCAAGTGTCACAGCGGCGAATGCATCACCCTGGACAAAGTCTGCAACA
  TGGCTAGAGACTGCCGGGACTGGTCAGATGAACCCATCAAAGAGTGCGGGACCAACGAAT
  GCTTGGACAACAACGGCGGCTGTTCCCACGTCTGCAATGACCTTAAGATCGGCTACGAGT
  GCCTGTGCCCCGACGGCTTCCAGCTGGTGGCCCAGCGAAGATGCGAAGATATCGATGAGT
  GTCAGGATCCCGACACCTGCAGCCAGCTCTGCGTGAACCTGGAGGGTGGCTACAAGTGCC
  AGTGTGAGGAAGGCTTCCAGCTGGACCCCCACACGAAGGCCTGCAAGGCTGTGGGCTCCA
  TCGCCTACCTCTTCTTCACCAACCGGCACGAGGTCAGGAAGATGACGCTGGACCGGAGCG
  AGTACACCAGCCTCATCCCCAACCTGAGGAACGTGGTCGCTCTGGACACGGAGGTGGCCA
  GCAATAGAATCTACTGGTCTGACCTGTCCCAGAGAATGATCTGCAGCACCCAGCTTGACA
  GAGCCCACGGCGTCTCTTCCTATGACACCGTCATCAGCAGAGACATCCAGGCCCCCGACG
  GGCTGGCTGTGGACTGGATCCACAGCAACATCTACTGGACCGACTCTGTCCTGGGCACTG
  TCTCTGTTGCGGATACCAAGGGCGTGAAGAGGAAAACGTTATTCAGGGAGAACGGCTCCA
  AGCCAAGGGCCATCGTGGTGGATCCTGTTCATGGCTTCATGTACTGGACTGACTGGGGAA
  CTCCCGCCAAGATCAAGAAAGGGGGCCTGAATGGTGTGGACATCTACTCGCTGGTGACTG
  AAAACATTCAGTGGCCCAATGGCATCACCCTAGATCTCCTCAGTGGCCGCCTCTACTGGG
  TTGACTCCAAACTTCACTCCATCTCAAGCATCGATGTCAACGGGGGCAACCGGAAGACCA
  TCTTGGAGGATGAAAAGAGGCTGGCCCACCCCTTCTCCTTGGCCGTCTTTGAGGACAAAG
  TATTTTGGACAGATATCATCAACGAAGCCATTTTCAGTGCCAACCGCCTCACAGGTTCCG
  ATGTCAACTTGTTGGCTGAAAACCTACTGTCCCCAGAGGATATGGTTCTCTTCCACAACC
  TCACCCAGCCAAGAGGAGTGAACTGGTGTGAGAGGACCACCCTGAGCAATGGCGGCTGCC
  AGTATCTGTGCCTCCCTGCCCCGCAGATCAACCCCCACTCGCCCAAGTTTACCTGCGCCT
  GCCCGGACGGCATGCTGCTGGCCAGGGACATGAGGAGCTGCCTCACAGAGGCTGAGGCTG
  CAGTGGCCACCCAGGAGACATCCACCGTCAGGCTAAAGGTCAGCTCCACAGCCGTAAGGA
  CACAGCACACAACCACCCGGCCTGTTCCCGACACCTCCCGGCTGCCTGGGGCCACCCCTG
  GGCTCACCACGGTGGAGATAGTGACAATGTCTCACCAAGCTCTGGGCGACGTTGCTGGCA
  GAGGAAATGAGAAGAAGCCCAGTAGCGTGAGGGCTCTGTCCATTGTCCTCCCCATCGTGC
  TCCTCGTCTTCCTTTGCCTGGGGGTCTTCCTTCTATGGAAGAACTGGCGGCTTAAGAACA
  TCAACAGCATCAACTTTGACAACCCCGTCTATCAGAAGACCACAGAGGATGAGGTCCACA
  TTTGCCACAACCAGGACGGCTACAGCTACCCCTCGAGACAGATGGTCAGTCTGGAGGATG
  ACGTGGCGTGAACATCTGCCTGGAGTCCCGTCCCTGCCCAGAACCCTTCCTGAGACCTCG
  CCAGCCTTGTTTTATTCAAAGACAGAGAAGACCAAAGCATTGCCTGCCAGAGCTTTGTTT
  TATATATTTATTCATCTGGGAGGCAGAACAGGCTTCGGACAGTGCCCATGCAATGGCTTG
  GGTTGGGATTTTGGTTTCTTCCTTTCCTCGTGAAGGATAAGAGAAACAGGCCCGGGGGGA
  CCAGGATGACACCTCCATTTCTCTCCAGGAAGTTTTGAGTTTCTCTCCACCGTGACACAA
  TCCTCAAACATGGAAGATGAAAGGGCAGGGGATGTCAGGCCCAGAGAAGCAAGTGGCTTT
  CAACACACAACAGCAGATGGCACCAACGGGACCCCCTGGCCCTGCCTCATCCACCAATCT
  CTAAGCCAAACCCCTAAACTCAGGAGTCAACGTGTTTACCTCTTCTATGCAAGCCTTGCT
  AGACAGCCAGGTTAGCCTTTGCCCTGTCACCCCCAAATCATGACCCACCCAGTGTCTTTC
  GAGGTGGGTTTGTACCTTCCTTAAGCCAGGAAAGGGATTCATGGCGTCGGAAATGATCTG
  GCTGAATCCGTGGTGGCACCGAGACCAAACTCATTCACCAAATGATGCCACTTCCCAGAG
  GCAGAGCCTGAGTCACCGGTCACCCTTAATATTTATTAAGTGCCTGAGACACCCGGTTAC
  CTTGGCCGTGAGGACACGTGGCCTGCACCCAGGTGTGGCTGTCAGGACACCAGCCTGGTG
  CCCGTCCTCCCGACCCCTACCCACTTCCATTCCCGTGGTCTCCTTGCACTTTCTCAGTTC
  AGAGTTGTACACTGTGTACATTTGGCATTTGTGTTATTATTTTGCACTGTTTTCTGTCGT
  GTGTGTTGGGATGGGATCCCAGGCCAGGGAAAGCCCGTGTCAATGAATGCCGGGGACAGA
  GAGGGGCAGGTTGACCGGGACTTCAAAGCCGTGATCGTGAATATCGAGAACTGCCATTGT
  CGTCTTTATGTCCGCCCACCTAGTGCTTCCACTTCTATGCAAATGCCTCCAAGCCATTCA
  CTTCCCCAATCTTGTCGTTGATGGGTATGTGTTTAAAACATGCACGGTGAGGCCGGGCGC
  AGTGGCXXXXXXXXXCCGTCTCTAAAAATACAAAAAATTAGCCGGGCGCGGTGGCGGGCA
  CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGTGTGAACCCGGGAAGCGG
  AGGTTGAAGTGAGCCGAGATTGCGCCACTGCAGTCCGCAGTCCGGCCTGGGCGACAGAGC
  GAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000527 unedited
  CGGATTTTGGTATACGACTCACTATAGGGCGGCCCGCGATTCGGCACCAGGNACACAGCA
  GGTCGTGATCCGGGTCGGGACACTGCCTGGCAGAGGCTGCGAGCATGGGGCCCTGGGGCT
  GGAAATTGCGCTGGACCGTCGCCTTGCTCCTCGCCGCGGCGGGGACTGCAGTGGGCGACA
  GATGCGAAAGAAACGAGTTCCAGTGCCAAGACGGGAAATGCATCTCCTACAAGTGGGTCT
  GCGATGGCAGCGCTGAGTGCCAGGATGGCTCTGATGAGTCCCAGGAGACGTGCTTGTCTG
  TCACCTGCAAATCCGGGGACTTCAGCTGTGGGGGCCGTGTCAACCGCTGCATTCCTCAGT
  TCTGGAGGTGCGATGGCCAAGTGGACTGCGACAACGGCTCAGACGAGCAAGGCTGTCCCC
  CCAAGACGTGCTCCCAGGACGAGTTTCGCTGCCACGATGGGAAGTGCATCTCTCGGCAGT
  TCGTCTGTGACTCAGACCGGGACTGCTTGGACGGCTCAGACGAGGCCTCCTGCCCGGTGC
  TCACCTGTGGTCCCGCCAGCTTCCAGTGCAACAGCTCCACCTGCATCCCCCAGCTGTGGG
  CCTGCGACAACGACCCCGACTGCGAAGATGGCTCGGATGAGTGGCCGCAGCGCTTGTAGG
  GTCTTTTACGTGTTCCAAGGGGGACAGTAGCCCCTGCTCGGCCTTCGAGTTCCACTGCCT
  AAGTGGGCGAGTGCATCCACTCCAGCTGGCGCTGGGAAATGGTGGCCCCCGACTGGCAGG
  ACANATCTGACGAGGAACCTGGGGCTGTGGCCACCTGTCGCCTGACGAATCCAGTGCTCT
  GATGGGAACTGCATCATGGCGCCGGCAGTTGACCGGGATTTGACTGCCAGCTGGCCNCNN
  NAAATTTGCTGGCTTATGGACACTTTGGAGCCCACAAGTTCAGTGCCACGGGCGAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000527 unedited
  NNNTTAAGCTCTGNNACCGCGGCCGCATNCTAGNGTCGAGTTTTTTTTTTTTTTTTTTTT
  GAAACGGAGTCTCGCTCTGTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTCGG
  CTCACTTCAACCTCCGCTTCCCGGGTTCACACCATTCTCCTGCCTCAGCCTCCCGAGTAG
  CTGGGACTACAGGTGCCCGCCACCGCGCCCGGCTAATTTTTTGTATTTTTAAGAGAACGG
  GGTTTCACCTTGTTAGCCAGGATGGGTCTCGATCTCCTGACCTCATGATCCACCCGGCTT
  TGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGGCCCCGGGCTCACCGTGCAT
  GGTTTAAACACATACCCATTAACGACCAGATATGGGAAATGAATGGCTTTGAAGCATTTG
  GATANAAGTGGAAAACCTTAAGGGGGCGGGCATTAAGACCACAATGGGAGGTCTTCGAGA
  TTTACGAAAACCAGGTTTGAAAAACCCCGGGAAAACTGTCCCCTTTCTGTGCCCCGGGAT
  TTATTTGGAACAGGGTTTTTCCTGGGCCGGGGATCCCCTCCCCACACCCCCCACCGGAAA
  AAGCGGCCAAAAAAAACACCAAAGGCCAAAGGGCCCAATGGGCCACTTCTTTACCTCGAA
  AAGGCCAGGGGACCCCCGGAATGGAAAAGGGTTAGGGGCCGGAAGAACGGGCCCAAACTG
  GGGGTCTTCAAAACCACCTGGGGGGAGGCCCCCTGTCCTCCCGGGCAAGGGAACCGGGGG
  TTCATGCCCCTTTTAAAAATTAGGGGGGACCGGGGCTCCAGCCTTTCTCCTGGGAAAAGG
  GCCATTTTGGGAAAAAAGATTGGTTCCGGGCCCCCCCGGTTTTCCCGAGAATTTTCCCGG
  CCGGGAACCCTTCCCGGGTTAAGGAAGGCCAACCCCCCTTGAAGAACTGGNTGGTCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000527
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000527.2, NP_000518.1
• RefSeq Size: 5175 bp
• RefSeq ORF: 2583 bp
• Locus ID: 3949
• UniProt ID: P01130
• Cytogenetics: 19p13.2
• Domains: ldl_recept_b, EGF_CA, ldl_recept_a, EGF, EGF
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Endocytosis
• Gene Summary: The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.