alpha Synuclein (SNCA) (NM_000345) Human Untagged Clone

CAT#: SC119919

SNCA (untagged)-Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha Synuclein
• Synonyms: NACP; PARK1; PARK4; PD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119919 sequence for NM_000345 edited (data generated by NextGen Sequencing)
  ATGGATGTATTCATGAAAGGACTTTCAAAGGCCAAGGAGGGAGTTGTGGCTGCTGCTGAG
  AAAACCAAACAGGGTGTGGCAGAAGCAGCAGGAAAGACAAAAGAGGGTGTTCTCTATGTA
  GGCTCCAAAACCAAGGAGGGAGTGGTGCATGGTGTGGCAACAGTGGCTGAGAAGACCAAA
  GAGCAAGTGACAAATGTTGGAGGAGCAGTGGTGACGGGTGTGACAGCAGTAGCCCAGAAG
  ACAGTGGAGGGAGCAGGGAGCATTGCAGCAGCCACTGGCTTTGTCAAAAAGGACCAGTTG
  GGCAAGAATGAAGAAGGAGCCCCACAGGAAGGAATTCTGGAAGATATGCCTGTGGATCCT
  GACAATGAGGCTTATGAAATGCCTTCTGAGGAAGGGTATCAAGACTACGAACCTGAAGCC
  TAA
  
  Clone variation with respect to NM_000345.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000345 unedited
  GGTCGAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGAAGGGGCCCA
  AGAGAGGGGGCGAGCGACCGAGCGCCGCGACGCGGAAGTGAGGTGCGTGCGGGCTGCAGC
  GCAGACCCCGGCCCGGCCCCTCCGAGAGCGTCCTGGGCGCTCCCTCACGCCTTGCCTTCA
  AGCCTTCTGCCTTTCCACCCTCGTGAGCGGAGAACTGGGAGTGGCCATTCGACGACAGTG
  TGGTGTAAAGGAATTCATTAGCCATGGATGTATTCATGAAAGGACTTTCAAAGGCCAAGG
  AGGGAGTTGTGGCTGCTGCTGAGAAAACCAAACAGGGTGTGGCAGAAGCAGCAGGAAAGA
  CAAAAGAGGGTGTTCTCTATGTAGGCTCCAAAACCAAGGAGGGAGTGGTGCATGGTGTGG
  CAACAGTGGCTGAGAAGACCAAAGAGCAAGTGACAAATGTTGGAGGAGCAGTGGTGACGG
  GTGTGACAGCAGTAGCCCAGAAGACAGTGGAGGGAGCAGGGAGCATTGCAGCAGCCACTG
  GCTTTGTCAAAAAGGACCAGTTGGGCAAGAATGAAGAAGGAGCCCCACAGGAAGGAATTC
  TGGAAGATATGCCTGTGGATCCTGACAATGAGGCTTATGAAATGCCTTCTGAGGAAGGGT
  ATCAAGACTACGAACCTGAAGCCTAAGAAATATCTTTGCTCCCAGTTTCTTGAGATCTGC
  TGACAGATGTTCCATCCTGTACAAGTGCTCAGTTCCAATGTGCCCAGTCATGACATTTCT
  CANAGTTTTTACAGTGTATCTCGAAAGTCTTCATCAGCAGTGATTGAAGTATCTGCACCT
  GCCCNCACTCAGCATTNNCGTGCTTCCCTTTCACTGNAGTGAATACATGGTAGCAGGTCC
  TTGTGTGCTGTGGATTNTGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000345 unedited
  GATGGAACCTCCATTGCCAGNANAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGNNTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTATTTTGGCAATGAAATAACGTTTTATTTTAATTC
  TCACCATTTATATACAAACCCAAGTGAATTATTCACATCGCAAAAGGGGAAAATTTCATA
  TTTAGTATTTATAGGGGCATAGTTTCATGCTCACATATTTTTAAGTTTTATATATTTTAA
  CAAATTTCACAATACGTCATTTTTCTTAAACAGTTTCATTAAAAGACACCTAAAAATCTT
  ATAATATATGATAGCAAATCACTAACAACTTTTGAACAACAGCAACAAAAAAATAGGGGG
  GATTTAAAAATAAGGGGGAGCCACTTAGGGGTTTTTAATTTGTTTTAACATCGTAGATTG
  AAGCCACAAAATCCACAGCACACAAAGACCCTGCTACCATGTATTCACTTCAGGGAAAGG
  GAACCACCGAAATGCTGAGTGGGGGCAGGTACAAATACTTCAATCACTGGTGATGGAAGA
  CTTCGAGATACACTGTAAAAACTTTGAGAAATGTCATGACTGGGCACATTGGAACTGAGC
  ACTTGTACAGTATGGAACATCTGTCAACAGATCTCAAAAAACTGGGAGCAAAGATATTTC
  TTAGGCTTCAGGTTCGGAGTCTTGATACCCTTCCTCAAAAGGCATTTCATAAGCCTTATT
  GTCAGGATACACAAGCATTTTTTTCCAAAATTCCTTCCTGTGGGGGCTCCTTCTTAATAC
  TTTGACAAACTGTTCTTTTTTGACAAAGCCAGGGGCTGTTGGAATGCTTCCTGCTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000345
• Insert Size: 423 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000345.2, NP_000336.1
• RefSeq Size: 1543 bp
• RefSeq ORF: 423 bp
• Locus ID: 6622
• UniProt ID: P37840
• Cytogenetics: 4q22.1
• Domains: Synuclein
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Parkinson's disease
• Gene Summary: Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1, also known as NACP140) is the longest transcript and encodes the longer isoform (NACP140). Variants 1, 2, and 3 all encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.