RPE65 (NM_000329) Human Untagged Clone

CAT#: SC119977

RPE65 (untagged)-Human retinal pigment epithelium-specific protein 65kDa (RPE65)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPE65
• Synonyms: BCO3; LCA2; mRPE65; p63; rd12; RP20; sRPE65
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119977 sequence for NM_000329 edited (data generated by NextGen Sequencing)
  ATGTCTATCCAGGTTGAGCATCCTGCTGGTGGTTACAAGAAACTGTTTGAAACTGTGGAG
  GAACTGTCCTCGCCGCTCACAGCTCATGTAACAGGCAGGATCCCCCTCTGGCTCACCGGC
  AGTCTCCTTCGATGTGGGCCAGGACTCTTTGAAGTTGGATCTGAGCCATTTTACCACCTG
  TTTGATGGGCAAGCCCTCCTGCACAAGTTTGACTTTAAAGAAGGACATGTCACATACCAC
  AGAAGGTTCATCCGCACTGATGCTTACGTACGGGCAATGACTGAGAAAAGGATCGTCATA
  ACAGAATTTGGCACCTGTGCTTTCCCAGATCCCTGCAAGAATATATTTTCCAGGTTTTTT
  TCTTACTTTCGAGGAGTAGAGGTTACTGACAATGCCCTTGTTAATGTCTACCCAGTGGGG
  GAAGATTACTACGCTTGCACAGAGACCAACTTTATTACAAAGATTAATCCAGAGACCTTG
  GAGACAATTAAGCAGGTTGATCTTTGCAACTATGTCTCTGTCAATGGGGCCACTGCTCAC
  CCCCACATTGAAAATGATGGAACCGTTTACAATATTGGTAATTGCTTTGGAAAAAATTTT
  TCAATTGCCTACAACATTGTAAAGATCCCACCACTGCAAGCAGACAAGGAAGATCCAATA
  AGCAAGTCAGAGATCGTTGTACAATTCCCCTGCAGTGACCGATTCAAGCCATCTTACGTT
  CATAGTTTTGGTCTGACTCCCAACTATATCGTTTTTGTGGAGACACCAGTCAAAATTAAC
  CTGTTCAAGTTCCTTTCTTCATGGAGTCTTTGGGGAGCCAACTACATGGATTGTTTTGAG
  TCCAATGAAACCATGGGGGTTTGGCTTCATATTGCTGACAAAAAAAGGAAAAAGTACCTC
  AATAATAAATACAGAACTTCTCCTTTCAACCTCTTCCATCACATCAACACCTATGAAGAC
  AATGGGTTTCTGATTGTGGATCTCTGCTGCTGGAAAGGATTTGAGTTTGTTTATAATTAC
  TTATATTTAGCCAATTTACGTGAGAACTGGGAAGAGGTGAAAAAAAATGCCAGAAAGGCT
  CCCCAACCTGAAGTTAGGAGATATGTACTTCCTTTGAATATTGACAAGGCTGACACAGGC
  AAGAATTTAGTCACGCTCCCCAATACAACTGCCACTGCAATTCTGTGCAGTGACGAGACT
  ATCTGGCTGGAGCCTGAAGTTCTCTTTTCAGGGCCTCGTCAAGCATTTGAGTTTCCTCAA
  ATCAATTACCAGAAGTATTGTGGGAAACCTTACACATATGCGTATGGACTTGGCTTGAAT
  CACTTTGTTCCAGATAGGCTCTGTAAGCTGAATGTCAAAACTAAAGAAACTTGGGTTTGG
  CAAGAGCCTGATTCATACCCATCAGAACCCATCTTTGTTTCTCACCCAGATGCCTTGGAA
  GAAGATGATGGTGTAGTTCTGAGTGTGGTGGTGAGCCCAGGAGCAGGACAAAAGCCTGCT
  TATCTCCTGATTCTGAATGCCAAGGACTTAAGTGAAGTTGCCCGGGCTGAAGTGGAGATT
  AACATCCCTGTCACCTTTCATGGACTGTTCAAAAAATCTTGA
  
  Clone variation with respect to NM_000329.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000329 unedited
  NAGGATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCAGAACTC
  TGGATCCTGAACTGGAAGAAAAGTCTATCCAGGTTGAGCATCCTGCTGGTGGTTACAAGA
  AACTGTTTGAAACTGTGGAGGAACTGTCCTCGCCGCTCACAGCTCATGTAACAGGCAGGA
  TCCCCCTCTGGCTCACCGGCAGTCTCCTTCGATGTGGGCCAGGACTCTTTGAAGTTGGAT
  CTGAGCCATTTTACCACCTGTTTGATGGGCAAGCCCTCCTGCACAAGTTTGACTTTAAAG
  AAGGACATGTCACATACCACAGAAGGTTCATCCGCACTGATGCTTACGTACGGGCAATGA
  CTGAGAAAAGGATCGTCATAACAGAATTTGGCACCTGTGCTTTCCCAGATCCCTGCAAGA
  ATATATTTTCCAGGTTTTTTTTCTTACTTTCGAGGAGTAGAGGTTACTGACAATGCCCTT
  GTTAATGTCTACCCAGTGGGGGAAGATTACTACGCTTGCACAGAGACCAACTTTATTACA
  AAGATTAATCCAGAGACCTTGGAGACAATTAAGCAGGTTGATCTTTGCAACTATGTCTCT
  GTCAATGGGGCCACTGCTCACCCCCACATTGAAAATGATGGAACCGTTTACAATATTGGT
  AATTGCTTTGGAAAAAATTTTTCAATTGCCTACAACATTGTAAAGATCCCACCACTGCAA
  GCAGACAAGGAAGATCCAATAAGCAAGTCAGAGATCGTTGTACAATTCCCCTGCAGTGAC
  CGATTCAAGCCATCTTACGTTCATAGTTNTGGTCTGACTCCCACTATATCGTTTTTGTGG
  AGACCCAGTCAAAATAACCTGTTCAGNTCCTTTCTCATGGAGTCTTTGGGAGCCACTACA
  TGGATGTTTGAGTCCATGAACCATGGGGTTTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000329 unedited
  GGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTAAAAACATTATTTTGGTCAATTTTTATTTTAAAACAATCTTTGGAAAAAAA
  AAATTTTAAAACCAAAGGGCTTAAAAGGTTTTAAAACCTGAGGGGTAATAATTAAATATT
  TTCCCCAAGGGATTGCCCAATGTTTAAATCCAATTAAACAAAAAAAATGCAATAAAGTTT
  AATCCTGGCACTTTACCCCAAAATTGGATTTTAAAAAAAATCACTTTTTTAAGCCTTTTA
  TGCTGTTTTTTAAAAAATAGCCACTACTTTTTGGCCTTGCTTTTATGGGACATAAGCTTT
  TAAAATAAAACTAAATCTCTGAAAAGTAATTTGGTGAAAAGGCCTAATTTGATTAAACAC
  AAATTTTTTTATTATAAAATCAAAAATAATTGCCCTTGCTTGTAAAAAACGGAAGGGTTA
  ACATTCATAAAATTCATAATTTTTGGGCAGTTCCGTATCCCCAAAATAATTACTGTAATA
  AAGCTTAAATAAAAGCCCCTGGTTAGAAAAAAAGTTGGGTTTGACATTTATTAATTAAGC
  CTTATGGCCGGATACTTCCTTGACTCAACAAAGCCACATATAATATAAATCAAGTGTAAA
  GGACTTTGGTTTTTAAATGTTAAAAATTAATGGGAGCAGATAGGCCCCTAAAATAGGCTC
  TTATAATAGGAATTAAAAGCCTTTTAGTAAGCCCACATTCATTTCCTATTTGATGGCTAA
  ATATTTAAGAGTTTTAACATTATGGCCTGTCTACAGAGGAAGTTGATTTCCAAATCGTAC
  TTTTTTTGAATAAGAGAATGTTGCTAACTCAGTGCTTTTGAAACATGCAAATTGGCCCTT
  TGCAGTAAACCTGACTATACGGCTAAATGAACAATTGGTTGCCACCTGACTGTTNTTCAT
  TTGCTACAAACAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000329
• Insert Size: 2780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000329.2, NP_000320.1
• RefSeq Size: 2608 bp
• RefSeq ORF: 1602 bp
• Locus ID: 6121
• UniProt ID: Q16518
• Cytogenetics: 1p31.3
• Domains: RPE65
• Protein Families: Druggable Genome
• Protein Pathways: Retinol metabolism
• Gene Summary: The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]