KCNH2 (NM_000238) Human Untagged Clone

CAT#: SC128170

KCNH2 (untagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNH2
• Synonyms: ERG-1; ERG1; H-ERG; HERG; HERG1; Kv11.1; LQT2; SQT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000238 edited
  GCCCGCCCGGCCCGCCCATGGGCTCAGGATGCCGGTGCGGAGGGGCCACGTCGCGCCGCA
  GAACACCTTCCTGGACACCATCATCCGCAAGTTTGAGGGCCAGAGCCGTAAGTTCATCAT
  CGCCAACGCTCGGGTGGAGAACTGCGCCGTCATCTACTGCAACGACGGCTTCTGCGAGCT
  GTGCGGCTACTCGCGGGCCGAGGTGATGCAGCGACCCTGCACCTGCGACTTCCTGCACGG
  GCCGCGCACGCAGCGCCGCGCTGCCGCGCAGATCGCGCAGGCACTGCTGGGCGCCGAGGA
  GCGCAAAGTGGAAATCGCCTTCTACCGGAAAGATGGGAGCTGCTTCCTATGTCTGGTGGA
  TGTGGTGCCCGTGAAGAACGAGGATGGGGCTGTCATCATGTTCATCCTCAATTTCGAGGT
  GGTGATGGAGAAGGACATGGTGGGGTCCCCGGCTCATGACACCAACCACCGGGGCCCCCC
  CACCAGCTGGCTGGCCCCAGGCCGCGCCAAGACCTTCCGCCTGAAGCTGCCCGCGCTGCT
  GGCGCTGACGGCCCGGGAGTCGTCGGTGCGGTCGGGCGGCGCGGGCGGCGCGGGCGCCCC
  GGGGGCCGTGGTGGTGGACGTGGACCTGACGCCCGCGGCACCCAGCAGCGAGTCGCTGGC
  CCTGGACGAAGTGACAGCCATGGACAACCACGTGGCAGGGCTCGGGCCCGCGGAGGAGCG
  GCGTGCGCTGGTGGGTCCCGGCTCTCCGCCCCGCAGCGCGCCCGGCCAGCTCCCATCGCC
  CCGGGCGCACAGCCTCAACCCCGACGCCTCGGGCTCCAGCTGCAGCCTGGCCCGGACGCG
  CTCCCGAGAAAGCTGCGCCAGCGTGCGCCGCGCCTCGTCGGCCGACGACATCGAGGCCAT
  GCGCGCCGGGGTGCTGCCCCCGCCACCGCGCCACGCCAGCACCGGGGCCATGCACCCACT
  GCGCAGCGGCTTGCTCAACTCCACCTCGGACTCCGACCTCGTGCGCTACCGCACCATTAG
  CAAGATTCCCCAAATCACCCTCAACTTTGTGGACCTCAAGGGCGACCCCTTCTTGGCTTC
  GCCCACCAGTGACCGTGAGATCATAGCACCTAAGATAAAGGAGCGAACCCACAATGTCAC
  TGAGAAGGTCACCCAGGTCCTGTCCCTGGGCGCCGACGTGCTGCCTGAGTACAAGCTGCA
  GGCACCGCGCATCCACCGCTGGACCATCCTGCATTACAGCCCCTTCAAGGCCGTGTGGGA
  CTGGCTCATCCTGCTGCTGGTCATCTACACGGCTGTCTTCACACCCTACTCGGCTGCCTT
  CCTGCTGAAGGAGACGGAAGAAGGCCCGCCTGCTACCGAGTGTGGCTACGCCTGCCAGCC
  GCTGGCTGTGGTGGACCTCATCGTGGACATCATGTTCATTGTGGACATCCTCATCAACTT
  CCGCACCACCTACGTCAATGCCAACGAGGAGGTGGTCAGCCACCCCGGCCGCATTGCCGT
  CCACTACTTCAAGGGCTGGTTCCTCATCGACATGGTGGCCGCCATCCCCTTCGACCTGCT
  CATCTTTGGCTCTGGCTCTGAGGAGCTGATCGGGCTGCTGAAGACTGCGCGGCTGCTGCG
  GCTGGTGCGCGTGGCGCGGAAGCTGGATCGCTACTCAGAGTACGGCGCGGCCGTGCTGTT
  CTTGCTCATGTGCACCTTTGCGCTCATCGCGCACTGGCTGGCCTGCATCTGGTACGCCAT
  CGGCAACATGGAGCAGCCACACATGGACTCACGCATCGGCTGGCTGCACAACCTGGGCGA
  CCAGATAGGCAAACCCTACAACAGCAGCGGCCTGGGCGGCCCCTCCATCAAGGACAAGTA
  TGTGACGGCGCTCTACTTCACCTTCAGCAGCCTCACCAGTGTGGGCTTCGGCAACGTCTC
  TCCCAACACCAACTCAGAGAAGATCTTCTCCATCTGCGTCATGCTCATTGGCTCCCTCAT
  GTACGCTAGCATCTTCGGCAACGTGTCGGCCATCATCCAGCGGCTGTACTCGGGCACAGC
  CCGCTACCACACACAGATGCTGCGGGTGCGGGAGTTCATCCGCTTCCACCAGATCCCCAA
  TCCCCTGCGCCAGCGCCTCGAGGAGTACTTCCAGCACGCCTGGTCCTACACCAACGGCAT
  CGACATGAACGCGGTGCTGAAGGGCTTCCCTGAGTGCCTGCAGGCTGACATCTGCCTGCA
  CCTGAACCGCTCACTGCTGCAGCACTGCAAACCCTTCCGAGGGGCCACCAAGGGCTGCCT
  TCGGGCCCTGGCCATGAAGTTCAAGACCACACATGCACCGCCAGGGGACACACTGGTGCA
  TGCTGGGGACCTGCTCACCGCCCTGTACTTCATCTCCCGGGGCTCCATCGAGATCCTGCG
  GGGCGACGTCGTCGTGGCCATCCTGGGGAAGAATGACATCTTTGGGGAGCCTCTGAACCT
  GTATGCAAGGCCTGGCAAGTCGAACGGGGATGTGCGGGCCCTCACCTACTGTGACCTACA
  CAAGATCCATCGGGACGACCTGCTGGAGGTGCTGGACATGTACCCTGAGTTCTCCGACCA
  CTTCTGGTCCAGCCTGGAGATCACCTTCAACCTGCGAGATACCAACATGATCCCGGGCTC
  CCCCGGCAGTACGGAGTTAGAGGGTGGCTTCAGTCGGCAACGCAAGCGCAAGTTGTCCTT
  CCGCAGGCGCACGGACAAGGACACGGAGCAGCCAGGGGAGGTGTCGGCCTTGGGGCCGGG
  CCGGGCGGGGGCAGGGCCGAGTAGCCGGGGCCGGCCGGGGGGGCCGTGGGGGGAGAGCCC
  GTCCAGTGGCCCCTCCAGCCCTGAGAGCAGTGAGGATGAGGGCCCAGGCCGCAGCTCCAG
  CCCCCTCCGCCTGGTGCCCTTCTCCAGCCCCAGGCCCCCCGGAGAGCCGCCGGGTGGGGA
  GCCCCTGATGGAGGACTGCGAGAAGAGCAGCGACACTTGCAACCCCCTGTCAGGCGCCTT
  CTCAGGAGTGTCCAACATTTTCAGCTTCTGGGGGGACAGTCGGGGCCGCCAGTACCAGGA
  GCTCCCTCGATGCCCCGCCCCCACCCCCAGCCTCCTCAACATCCCCCTCTCCAGCCCGGG
  TCGGCGGCCCCGGGGCGACGTGGAGAGCAGGCTGGATGCCCTCCAGCGCCAGCTCAACAG
  GCTGGAGACCCGGCTGAGTGCAGACATGGCCACTGTCCTGCAGCTGCTACAGAGGCAGAT
  GACGCTGGTCCCGCCCGCCTACAGTGCTGTGACCACCCCGGGGCCTGGCCCCACTTCCAC
  ATCCCCGCTGTTGCCCGTCAGCCCCCTCCCCACCCTCACCTTGGACTCGCTTTCTCAGGT
  TTCCCAGTTCATGGCGTGTGAGGAGCTGCCCCCGGGGGCCCCAGAGCTTCCCCAAGAAGG
  CCCCACACGACGCCTCTCCCTACCGGGCCAGCTGGGGGCCCTCACCTCCCAGCCCCTGCA
  CAGACACGGCTCGGACCCGGGCAGTTAGTGGGGCTGCCCAGTGTGGACACGTGGCTCACC
  CAGGGATCAAGGCGCTGCTGGGCCGCTCCCCTTGGAGGCCCTGCTCAGGAGGCCCTGACC
  GTGGAAGGGGAGAGGAACTCGAAAGCACAGCTCCTCCCCCAGCCCTTGGGACCATCTTCT
  CCTGCAGTCCCCTGGGCCCCAGTGAGAGGGGCAGGGGCAGGGCCGGCAGTAGGTGGGGCC
  TGTGGTCCCCCCACTGCCCTGAGGGCATTAGCTGGTCTAACTGCCCGGAGGCACCCGGCC
  CTGGGCCTTAGGCACCTCAAGGACTTTTCTGCTATTTACTGCTCTTATTGTTAAGGATAA
  TAATTAAGGATCATATGAATAATTAATGAAGATGCTGATGACTATGAATAATAAATAATT
  ATCCTGAGGAGACTCCAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000238 unedited
  NNAAGGTTCGNCATTTGTNATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCC
  GCCCGGCCCGCCCATGGGCTCAGATGCCGGTGCGGAGGGGCCACGTCGCGCCGCAGACAC
  CTTCCTGGACACCATCATCCGCAAGTTTGAGGGCCAGAGCCGTAAGTTCATCATCGCCAA
  CGCTCGGGTGGAGAACTGCGCCGTCATCTACTGCAACGACGGCTTCTGCGAGCTGTGCGG
  CTACTCGCGGGCCGAGGTGATGCAGCGACCCTGCACCTGCGACTTCCTGCACGGGCCGCG
  CACGCAGCGCCGCGCTGCCGCGCAGATCGCGCAGGCACTGCTGGGCGCCGAGGAGCGCAA
  AGTGGAAATCGCCTTCTACCGGAAAGATGGGAGCTGCTTCCTATGTCTGGTGGATGTGGT
  GCCCGTGAAGAACGAGGATGGGGCTGTCATCATGTTCATCCTCAATTTCGAGGTGGTGAT
  GGAGAAGGACATGGTGGGGTCCCCGGCTCATGACACCAACCACCGGGGCCCCCCCACCAG
  CTGGCTGGCCCCAGGCCGCGCCAAGACCTTCCGCCTGAAGCTGCCCGCGCTGCTGGCGCT
  GACGGCCCGGGAGTCGTCGGTGCGGTCGGGCGGCGCGGGCGGCGCGGGCGCCCCGGGGGC
  CGTGGTGGTGGACGTGGACCTGACGCCCGCGGCACCCAGCAGCGAGTCGCTGGCCCTGGA
  CGAAGTGACAGCCATGGACAACCACGTGGCAGGGCTCGGGCCCGCGGAGGAGCGGCGTGC
  GCTGGTGGGTCCCGGCTCTCCGCCCCGCAGCGCGCCCGGCCAGCTCCCATCGCCCCGGGC
  GCACAGCCCTCACCCCCGACGCCTCGGGCTCCAGCTGCAGCCTGGCCCGGACGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000238 unedited
  NCCGGCATGGNGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATGC
  CACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTGGAGTCTCCTCAGGATAATTATTTATTATTCATA
  GTCATCAGCATCTTCATTAATTATTCATATGATCCTTAATTATTATCCTTAACAATAAGA
  GCAGTAAATAGCAGAAAAGTCCTTGAGGTGCCTAAGGCCCAGGGCCGGGTGCCTCCGGGC
  AGTTAGACCAGCTAATGCCCTCAGGGCAGTGGGGGGACCACAGGCCCCACCTACTGCCGG
  CCCTGCCCCTGCCCCTCTCACTGGGGCCCAGGGGACTGCAGGAGAAGATGGTCCCAAGGG
  CTGGGGGAGGAGCTGTGCTTTCGAGTTCCTCTCCCCTTCCACGGTCAGGGCCTCCTGAGC
  AGGGCCTCCAAGGGGAGCGGCCCAGCAGCGCCTTGATCCCTGGGTGAGCCACGTGTCCAC
  ACTGGGCAGCCCCACTAACTGCCCGGGTCCGAGCCGTGTCTGTGCAGGGGCTGGGAGGTG
  AGGGCCCCCANCTGGCCCGGTAGGGAGAGGCGTCCTGTGGGGCCTTCTTGGGGAAGCTCT
  GGGGCCCCCGGGGGCAGCTCCTCACACGCCATGAACTGGGAAACCTGAGAAAGCGAGTCC
  AAGGTGAGGGTGGGGAGGGGGCTGACGGGCAACAGCGGGGATGTGGAAGTGGGGCCAGGC
  CCCGGGGTGGTCACAGCACTGTAGGCGGGCGGGACCAGCGTCATCTGCCTCTGTAGCAGC
  TGCAAGACAGTGGCCATGTCTGCACTCAGCCGGGTCTCCAGCCTGTTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_000238
• Insert Size: 4300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000238.2, NP_000229.1
• RefSeq Size: 3900 bp
• RefSeq ORF: 3480 bp
• Locus ID: 3757
• UniProt ID: Q12809
• Cytogenetics: 7q36.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transcription Factors, Transmembrane
• Gene Summary: This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (a).