Gelsolin (GSN) (NM_000177) Human Untagged Clone

CAT#: SC120033

GSN (untagged)-Human gelsolin (GSN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Gelsolin
• Synonyms: ADF; AGEL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000177, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCCGCACCGCCCCGCGCCCGCGCTGCTTTGCGCGCTGTCCCTGGCGCTGTGCGCGCTGTCGCTGC
  CCGTCCGCGCGGCCACTGCGTCGCGGGGGGCGTCCCAGGCGGGGGCGCCCCAGGGGCGGGTGCCCGAGGC
  GCGGCCCAACAGCATGGTGGTGGAACACCCCGAGTTCCTCAAGGCAGGGAAGGAGCCTGGCCTGCAGATC
  TGGCGTGTGGAGAAGTTCGATCTGGTGCCCGTGCCCACCAACCTTTATGGAGACTTCTTCACGGGCGACG
  CCTACGTCATCCTGAAGACAGTGCAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTGGG
  CAATGAGTGCAGCCAGGATGAGAGCGGGGCGGCCGCCATCTTTACCGTGCAGCTGGATGACTACCTGAAC
  GGCCGGGCCGTGCAGCACCGTGAGGTCCAGGGCTTCGAGTCGGCCACCTTCCTAGGCTACTTCAAGTCTG
  GCCTGAAGTACAAGAAAGGAGGTGTGGCATCAGGATTCAAGCACGTGGTACCCAACGAGGTGGTGGTGCA
  GAGACTCTTCCAGGTCAAAGGGCGGCGTGTGGTCCGTGCCACCGAGGTACCTGTGTCCTGGGAGAGCTTC
  AACAATGGCGACTGCTTCATCCTGGACCTGGGCAACAACATCCACCAGTGGTGTGGTTCCAACAGCAATC
  GGTATGAAAGACTGAAGGCCACACAGGTGTCCAAGGGCATCCGGGACAACGAGCGGAGTGGCCGGGCCCG
  AGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAGGTGCTGGGCCCCAAGCCGGCTCTG
  CCTGCAGGTACCGAGGACACCGCCAAGGAGGATGCGGCCAACCGCAAGCTGGCCAAGCTCTACAAGGTCT
  CCAATGGTGCAGGGACCATGTCCGTCTCCCTCGTGGCTGATGAGAACCCCTTCGCCCAGGGGGCCCTGAA
  GTCAGAGGACTGCTTCATCCTGGACCACGGCAAAGATGGGAAAATCTTTGTCTGGAAAGGCAAGCAGGCA
  AACACGGAGGAGAGGAAGGCTGCCCTCAAAACAGCCTCTGACTTCATCACCAAGATGGACTACCCCAAGC
  AGACTCAGGTCTCGGTCCTTCCTGAGGGCGGTGAGACCCCACTGTTCAAGCAGTTCTTCAAGAACTGGCG
  GGACCCAGACCAGACAGATGGCCTGGGCTTGTCCTACCTTTCCAGCCATATCGCCAACGTGGAGCGGGTG
  CCCTTCGACGCCGCCACCCTGCACACCTCCACTGCCATGGCCGCCCAGCACGGCATGGATGACGATGGCA
  CAGGCCAGAAACAGATCTGGAGAATCGAAGGTTCCAACAAGGTGCCCGTGGACCCTGCCACATATGGACA
  GTTCTATGGAGGCGACAGCTACATCATTCTGTACAACTACCGCCATGGTGGCCGCCAGGGGCAGATAATC
  TATAACTGGCAGGGTGCCCAGTCTACCCAGGATGAGGTCGCTGCATCTGCCATCCTGACTGCTCAGCTGG
  ATGAGGAGCTGGGAGGTACCCCTGTCCAGAGCCGTGTGGTCCAAGGCAAGGAGCCCGCCCACCTCATGAG
  CCTGTTTGGTGGGAAGCCCATGATCATCTACAAGGGCGGCACCTCCCGCGAGGGCGGGCAGACAGCCCCT
  GCCAGCACCCGCCTCTTCCAGGTCCGCGCCAACAGCGCTGGAGCCACCCGGGCTGTTGAGGTATTGCCTA
  AGGCTGGTGCACTGAACTCCAACGATGCCTTTGTTCTGAAAACCCCCTCAGCCGCCTACCTGTGGGTGGG
  TACAGGAGCCAGCGAGGCAGAGAAGACGGGGGCCCAGGAGCTGCTCAGGGTGCTGCGGGCCCAACCTGTG
  CAGGTGGCAGAAGGCAGCGAGCCAGATGGCTTCTGGGAGGCCCTGGGCGGGAAGGCTGCCTACCGCACAT
  CCCCACGGCTGAAGGACAAGAAGATGGATGCCCATCCTCCTCGCCTCTTTGCCTGCTCCAACAAGATTGG
  ACGTTTTGTGATCGAAGAGGTTCCTGGTGAGCTCATGCAGGAAGACCTGGCAACGGATGACGTCATGCTT
  CTGGACACCTGGGACCAGGTCTTTGTCTGGGTTGGAAAGGATTCTCAAGAAGAAGAAAAGACAGAAGCCT
  TGACTTCTGCTAAGCGGTACATCGAGACGGACCCAGCCAATCGGGATCGGCGGACGCCCATCACCGTGGT
  GAAGCAAGGCTTTGAGCCTCCCTCCTTTGTGGGCTGGTTCCTTGGCTGGGATGATGATTACTGGTCTGTG
  GACCCCTTGGACAGGGCCATGGCTGAGCTGGCTGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000177 unedited
  AGGTCACATTTTGTAAACGACTCACTATAGGGCGGCCGCGAATTCGCACNGGGGCTGCCG
  ACTGGGTCCCCTGCCGCTGTCGCCACCATGGCTCCGCACCGCCCCGCGCCCGCGCTGCTT
  TGCGCGCTGTCCCTGGCGCTGTGCGCGCTGTCGCTGCCCGTCCGCGCGGCCACTGCGTCG
  CGGGGGGCGTCCCAGGCGGGGGCGCCCCAGGGGCGGGTGCCCGAGGCGCGGCCCAACAGC
  ATGGTGGTGGAACACCCCGAGTTCCTCAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGG
  CGTGTGGAGAAGTTCGATCTGGTGCCCGTGCCCACCAACCTTTATGGAGACTTCTTCACG
  GGCGACGCCTACGTCATCCTGAAGACAGTGCAGCTGAGGAACGGAAATCTGCAGTATGAC
  CTCCACTACTGGCTGGGCAATGAGTGCAGCCAGGATGAGAGCGGGGCGGCCGCCATCTTT
  ACCGTGCAGCTGGATGACTACCTGAACGGCCGGGCCGTGCAGCACCGTGAGGTCCAGGGC
  TTCGAGTCGGCCACCTTCCTAGGCTACTTCAAGTCTGGCCTGAAGTACAAGAAAGGAGGT
  GTGGCATCAGGATTCAAGCACGTGGTACCCAACGAGGTGGTGGTGCAGAGACTCTTCCAG
  GTCAAAGGGCGGCGTGTGGTCCGTGCCACCGAGGTACCTGTGTCCTGGGAGAGCTTCAAC
  AATGGCGACTGCTTCATCCTGGACCTGGGCAACAACATCCACCAGTGGTGTGGTTCCAAC
  AGCAAATCGTATGAAAGACTGAANGCCACACNAGTGTCCAAGGGCATNCGGGACACGAGC
  CGAGTGGCCGGGCCCGAGTGCACGTGTCTGGAGAGGGCACTGAGCCCGAGGCGATGCCTC
  AGGTGCTGGGCCCCAAGCCGGCTCTGCCCTGAAGTACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000177 unedited
  GGACGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCACACTTCAAAATGTT
  TTTATTGGATTTAATTTCCCAAACATTGACATTTTAAACAATTTTGCAAGGACTCTGAAT
  TTTTGCAGGGCTATTTTTGGATACTGTAAAAAAAAAAAAAAGAAACAACACACACACACA
  CACACACTCATAGCAGAGCTGCTCTGCTCGCTCTAAGGCCTCTTTGAGGGAAGGACAGTT
  CCAAAAGGCACTGACCGGTGACATGGGTGGGCCCTGCCCCTCCTCAGGCAGCCAGCTCAG
  CCATGGCCCTGTCCAAGGGGTCCACAGACCAGTAATCATCATCCCAGTCAAGGAACCAGC
  CCACAAAGGAGGGAGGCTCAAAGCCTTGCTTAACCACGGTGATGGGCGTCCGCCGATCCC
  GATTGGCTGGGACCGCCTCGATGTACCGTTTAGCACAAGCCAAGGCTTCCGTCTTCCCTT
  CTTCTTGAGAATCCTTTACAACCCACACAAAGACCTGGTCCCAGCTGTCCAGAAGCATGA
  CGCCATCCCGCGCCAGGACTTCCTGCATGAGCTCACCAGGAACCTCTTTCATCACAAAAC
  GTTCAATCTTGTTGGAGCAGGCAAAGAGGCGAGGAGGATGGCCATCCATCTTCTTGTCCT
  TCAACCGTGGGGATGCGCGTAAGGCAAACCTCCCGCCACGGCCTCCCAGAAGCATCTGGC
  TCGCTGCCTTCTGCCACCTGCAAGGTTGCGCCCGCACACCCTGAGAACTCCTGGCCCCCG
  CTTTCTCTGCCTTGCTGCTCCTGACCCACCCCCAGGTAGTCGGCTGAAGAGGCTCTAAGA
  ACAAAGCCCCGGTGGAGTTATTGCACCACCTCTAGAATAACTAAAAGACCCGGCGGTTCC
  ACCCTTGTGTCCGTACCCGTAAAACTGGCCCTGCAAGGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000177
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000177.3, NP_000168.1
• RefSeq Size: 2705 bp
• RefSeq ORF: 2349 bp
• Locus ID: 2934
• UniProt ID: P06396
• Cytogenetics: 9q33.2
• Domains: GEL, Gelsolin
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (a).