GJA1 (NM_000165) Human Untagged Clone

CAT#: SC125548

GJA1 (untagged)-Human gap junction protein, alpha 1, 43kDa (GJA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GJA1
• Synonyms: AVSD3; CMDR; CX43; EKVP; EKVP3; GJAL; HLHS1; HSS; ODDD; PPKCA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125548 sequence for NM_000165 edited (data generated by NextGen Sequencing)
  ATGGGTGACTGGAGCGCCTTAGGCAAACTCCTTGACAAGGTTCAAGCCTACTCAACTGCT
  GGAGGGAAGGTGTGGCTGTCAGTACTTTTCATTTTCCGAATCCTGCTGCTGGGGACAGCG
  GTTGAGTCAGCCTGGGGAGATGAGCAGTCTGCCTTTCGTTGTAACACTCAGCAACCTGGT
  TGTGAAAATGTCTGCTATGACAAGTCTTTCCCAATCTCTCATGTGCGCTTCTGGGTCCTG
  CAGATCATATTTGTGTCTGTACCCACACTCTTGTACCTGGCTCATGTGTTCTATGTGATG
  CGAAAGGAAGAGAAACTGAACAAGAAAGAGGAAGAACTCAAGGTTGCCCAAACTGATGGT
  GTCAATGTGGACATGCACTTGAAGCAGATTGAGATAAAGAAGTTCAAGTACGGTATTGAA
  GAGCATGGTAAGGTGAAAATGCGAGGGGGGTTGCTGCGAACCTACATCATCAGTATCCTC
  TTCAAGTCTATCTTTGAGGTGGCCTTCTTGCTGATCCAGTGGTACATCTATGGATTCAGC
  TTGAGTGCTGTTTACACTTGCAAAAGAGATCCCTGCCCACATCAGGTGGACTGTTTCCTC
  TCTCGCCCCACGGAGAAAACCATCTTCATCATCTTCATGCTGGTGGTGTCCTTGGTGTCC
  CTGGCCTTGAATATCATTGAACTCTTCTATGTTTTCTTCAAGGGCGTTAAGGATCGGGTT
  AAGGGAAAGAGCGACCCTTACCATGCGACCAGTGGTGCGCTGAGCCCTGCCAAAGACTGT
  GGGTCTCAAAAATATGCTTATTTCAATGGCTGCTCCTCACCAACCGCTCCCCTCTCGCCT
  ATGTCTCCTCCTGGGTACAAGCTGGTTACTGGCGACAGAAACAATTCTTCTTGCCGCAAT
  TACAACAAGCAAGCAAGTGAGCAAAACTGGGCTAATTACAGTGCAGAACAAAATCGAATG
  GGGCAGGCGGGAAGCACCATCTCTAACTCCCATGCACAGCCTTTTGATTTCCCCGATGAT
  AACCAGAATTCTAAAAAACTAGCTGCTGGACATGAATTACAGCCACTAGCCATTGTGGAC
  CAGCGACCTTCAAGCAGAGCCAGCAGTCGTGCCAGCAGCAGACCTCGGCCTGATGACCTG
  GAGATCTAG
  
  Clone variation with respect to NM_000165.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000165 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGC
  ACGAGTATCAGCACTTTTCTTTCATTAGGGGGAAGGCGTGAGGAAAGTACCAAACAGCAG
  CGGAGTTTTAAACTTTAAATAGACAGGTCTGAGTGCCTGAACTTGCCTTTTCATTTTACT
  TCATCCTCCAAGGAGTTCAATCACTTGGCGTGACTTCACTACTTTTAAGCAAAAGAGTGG
  TGCCCAGGCAACATGGGTGACTGGAGCGCCTTAGGCAAACTCCTTGACAAGGTTCAAGCC
  TACTCAACTGCTGGAGGGAAGGTGTGGCTGTCAGTACTTTTCATTTTCCGAATCCTGCTG
  CTGGGGACAGCGGTTGAGTCAGCCTGGGGAGATGAGCAGTCTGCCTTTCGTTGTAACACT
  CAGCAACCTGGTTGTGAAAATGTCTGCTATGACAAGTCTTTCCCAATCTCTCATGTGCGC
  TTCTGGGTCCTGCAGATCATATTTGTGTCTGTACCCACACTCTTGTACCTGGCTCATGTG
  TTCTATGTGATGCGAAAGGAAGAGAAACTGAACAAGAAAGAGGAAGAACTCAAGGTTGCC
  CAACTGATGGTGTCAATGTGGACATGCACTTGAAGCAGATCGAATAAAAGAAGTTCAAGT
  ACGGTATTGAAGAGCATGGNTAGGTGAAATGCGAGGGGGGGGGTTGCTGCNAACCTACAT
  CATCAGTATCCTCTTCAGTCATTTTTTGAGGGTGCCTCTTGCTGATNCCAGTGGACATCT
  ATGGATTCNCTTGGGAGGGCTGGTTACACTGCAAAAAAGACCCTGCACATCAGGGGGGGC
  CGTGTTCCTCTCTCGCCCACGGAGAAACATCTCATATTCTGCCGGGGGGGGGGCNCCGTG
  GGGGCCCTGGCCTGAAATAAATGACTCTTTTTGTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000165 unedited
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTATACTAAATTAAAACTT
  TATTGAATAAAGAACACTCTCCAGAACACATGATCTGATGGACTAGGTCTACATTACATG
  CAATGAAGCTGAACATGACCGTAGTTTAACATGGAATGTCAAACAAATTAGTAATTTTCA
  TTGTACAAAACTGTTTATGTAGCTGACATGCATGCAAGAAGAAAAGTGTCATACTCAGCA
  TTTTCACCAATCTTCTGTGATGAGTTTTAAAAAAGGCATTTGGAGAAACTGGTAGATGTC
  TTCAACATATAGTTCAAATAAATTAGTTGTATGTGTACTACTGAATCCTCTTTCAACCTC
  TCTTGCATTCCAAATAAAATCTTAGTGCAAACATCAAAGTTGCTGGTCACTCCAAAAGAC
  TGCTAAACTCATAATAGAATACAAGTTCTGAATTACTGTGTATTAAGTAGGAAAACCCAA
  GAATACCAGTTACTGCAAAATTCATTTAGGCACATTTAATAGTCCACTCTAAGCATTTTT
  TCTAAGTCTCTCACATTAATGACTAAAGCCTGTTTACAGTACTAAAATTCTATCATTCAA
  GCATCAATGATTACATTTCAGAAAAAGGAAATTCATTTCAGTAATAGCATTACTGCTATA
  TCCTANAAAAAGGAGAGAAAATAAAGAATCATAATTAACAAAACCAGTATCCATAATAGG
  ATACACAGGGGAAAAACATTNTAGGAGAAANAAAAAGTGTGCTTTACTTGCCCAGCANGA
  ATATATAATGTAAACACATATTGGCCACACACAAAAATCTGAGAAGGTTNCAATTTGACC
  CATAGTGCATATTTTCAAACTGNGTTGNGAGTTTACTGATCTGGCTGACATACTGACAAC
  AAACAACTCTCAACACTATATGATGGTATATAGCTGGCTGTTCCTGACTTACTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000165
• Insert Size: 2940 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000165.2, NP_000156.1
• RefSeq Size: 3088 bp
• RefSeq ORF: 1149 bp
• Locus ID: 2697
• UniProt ID: P17302
• Cytogenetics: 6q22.31
• Domains: CNX, Connexin43
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction
• Gene Summary: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]