OriGene Logo
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Destination Vector pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro

pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro, Lenti vector with EF1a promoter, N-terminal HA tag, C-terminal Myc-DDK tag and IRES-Puro, 10 ug

Specifications Custom Cloning Product Manual FAQs
Catalog No. Description Price Availability  
PS100106 pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro, Lenti vector with EF1a promoter, N-terminal HA tag, C-terminal Myc-DDK tag and IRES-Puro, 10 ug $710 In Stock
TA50011-100 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, 100ul $248 In Stock
Add to Shopping Cart

Features:

This Lenti vector is a third generation lentiviral vector.

  • All OriGene's TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with N-terminal HA tag and C-terminal Myc-DDK tag.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided.
  • E. Coli. selection: Chloramphemicol (34 ug/ml)
  • Download full vector sequence
pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro, Lenti vector with EF1a promoter, N-terminal HA tag, C-terminal Myc-DDK tag and IRES-Puro, 10 ug Vector Image

Schematic of the multiple cloning sites:

pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro, Lenti vector with EF1a promoter, N-terminal HA tag, C-terminal Myc-DDK tag and IRES-Puro, 10 ug MCS Image

 

pattern
Inc 5000 Healthcare Company