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Home Destination Vector pLenti-EF1a-C-Myc-DDK-IRES-Puro     

pLenti-EF1a-C-Myc-DDK-IRES-Puro , Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro  , 10ug

Specifications Custom Cloning Product Manual FAQs
Catalog No. Description Price Availability  
PS100085 pLenti-EF1a-C-Myc-DDK-IRES-Puro , Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro  , 10ug $670 In Stock
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Features:

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The expression of the cloned ORF is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • A brief lentiviral packaging protocol is provided
  • E. Coli selection: Chloramphenicol(34ug/ml)
pLenti-EF1a-C-Myc-DDK-IRES-Puro , Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro  , 10ug Vector Image
Schematic of the multiple cloning sites:
pLenti-EF1a-C-Myc-DDK-IRES-Puro , Lenti Vector with EF1 alpha promoter and bicistronic IRES-Puro  , 10ug MCS Image
Download full vector sequence

 

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