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Home Destination Vector pEX-C-GST

PrecisionShuttle bacterial expression vector with C-terminal GST tag, 10 ug

Specifications Custom Cloning Product Manual FAQs
Catalog No. Description Price Availability  
PS100083 PrecisionShuttle bacterial expression vector with C-terminal GST tag, 10 ug $360 In Stock
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Features:

This vector is for E.coli expression.

  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal GST tag.
  • Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector.
  • Detection and purification of the transgene are best done using an anti-GST antibody.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal GST-tagging vector, PS100029
PrecisionShuttle bacterial expression vector with C-terminal GST tag, 10 ug Vector Image
Schematic of the multiple cloning sites:
PrecisionShuttle bacterial expression vector with C-terminal GST tag, 10 ug MCS Image
Download full vector sequence

 

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