Home Destination Vector pCMV6-AN-3DDK
PrecisionShuttle mammalian vector with N-terminal 3XDDK tag, 10 ug
- ORFs cloned in this vector will be expressed in mammalian cells as fusion proteins with 3 tandem DDK-tags on the C-terminal ends, resulting in 100X higher detection sensitivity than 1XDDK tag
- The 3DDK tag will increase the affinity of the fusion protein to anti-DDK tags and facilitate detection. This is especially useful for proteins of low expression level.
- If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal 3DDK-tagging vector, PS100057.
Schematic of the multiple cloning sites: