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Home Destination Vector pCMV6-AN-FC

PrecisionShuttle mammalian vector with N-terminal FC tag, 10 ug

Specifications Custom Cloning Product Manual FAQs
Catalog No. Description Price Availability  
PS100055 PrecisionShuttle mammalian vector with N-terminal FC tag, 10 ug $430 In Stock
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Features:
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal murine Fc tag.
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice
  • If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal Fc-tagging vector, PS100053.
PrecisionShuttle mammalian vector with N-terminal FC tag, 10 ug Vector Image
Schematic of the multiple cloning sites:
PrecisionShuttle mammalian vector with N-terminal FC tag, 10 ug MCS Image
Download full vector sequence

 

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