Silencing hsp25/hsp27 Gene Expression Augments Proteasome Activity and Increases CD8+ T-Cell–Mediated Tumor Killing and Memory Responses
Cancer Prevention Research, Jan 2012; 5: 122 - 137.
[HSPB1
]
Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells
Mol. Cancer Res., Jan 2012; 10: 133 - 142.
[SREBF1
]
Androgen Deprivation Causes Epithelial–Mesenchymal Transition in the Prostate: Implications for Androgen-Deprivation Therapy
Cancer Res., Jan 2012; 72: 527 - 536.
[AR
]
CB2 Cannabinoid Receptors Promote Neural Progenitor Cell Proliferation via mTORC1 Signaling
J. Biol. Chem., Jan 2012; 287: 1198 - 1209.
[Cnr2
]
ORFs cloned in this vector will be expressed in mammalian cells as a secreted tagged protein with a C-terminal murine Fc tag. Due to the presence of an N-terminal secretion signal sequence, the fusion protein will be secreted and this facilitates the downstream purification
The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
Easy purification using protein A or protein G affinity chromatography.
Increased solubility.
Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Fc-tagging vector, PS100056
